Effects of vitro sucrose on quality components of tea plants (Camellia sinensis) based on transcriptomic and metabolic analysis

Qian, Yumei; Zhang, Shuxiang; Yao, Shengbo; Xia, Jinxin; Li, Yanzhi; Dai, Xinlong; Wang, Wenzhao; Jiang, Xiaolan; Li, Yajun; Li, Mingzhuo; Gao, Liping; Xia, Tao

doi:10.1186/s12870-018-1335-0

Cited by 37 publications

(25 citation statements)

References 105 publications

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“…Phenolic synthesis requires carbohydrates, which explains the sharp increase in phenolic concentration in the presence of high sucrose observed in this experiment. A similar effect was also observed in Camellia sinensis plants in vitro (Qian et al 2018). Therefore, sucrose might play a role in birch in vitro shoot recalcitrance and lowered sucrose concentration might be beneficial during rejuvenation.…”

Section: Discussionsupporting

confidence: 70%

Peroxidase, polyphenol oxidase activity and total phenolic concentration in birch (Betula pendula) in vitro shoots during rejuvenation

2019

EEB

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Tissue recalcitrance remains a major problem limiting woody plant propagation using in vitro biotechnology methods. Increased generation of reactive oxygen species and high phenol concentration in woody tissues are considered as the main reasons for tissue recalcitrance in tissue culture. In this study, oxidative enzyme activity and total phenolic concentration were analyzed in vitro in both rejuvenated and mature shoots of birch. The effect of two factors-subculture time and medium sucrose concentration-on peroxidase and polyphenol oxidase activity, and total phenolic concentration in rejuvenated shoots were determined as possible rejuvenationaffecting factors. The results showed that mature shoots had increased peroxidase and polyphenol oxidase activity in shoot apices and stems but higher phenolic concentration in leaves as compared to rejuvenated shoots. Prolonged subculture time had significant effect on peroxidase activity and polyphenol oxidase activity in shoot apices, and thus this factor did not affect maturation. However, high sucrose concentration significantly increased total phenolic concentration in leaves and polyphenol oxidase activity in all plant parts, thus enhancing shoot maturation and recalcitrance in vitro.

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Section: Discussionsupporting

confidence: 70%

Peroxidase, polyphenol oxidase activity and total phenolic concentration in birch (Betula pendula) in vitro shoots during rejuvenation

2019

EEB

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“…In this study, the transcript levels of the CsWD40 gene were significantly upregulated under sucrose and ABA stresses, but no response was found to mannitol, NaCl, and SA (Salicylicacid) stresses under different temperatures. Our previous study showed that under ABA and sucrose stresses the most flavonoid was accumulated and the related gene expression increased [ 43 ]. This result suggests CsWD40 promotes the accumulation of products of the flavonoid biosynthetic pathway, which led to stress tolerance in tea plants.…”

Section: Discussionmentioning

confidence: 99%

“…The primers used are listed in Table S1 . The vectors were constructed and the genetic transformation of tobacco was performed according to the method of Li et al [ 43 ]. The phenotypes of transgenic plants growing in the controlled environment chamber were recorded to characterize the effect of transgene overexpression on growth.…”

Section: Methodsmentioning

confidence: 99%

A WD40 Repeat Protein from Camellia sinensis Regulates Anthocyanin and Proanthocyanidin Accumulation through the Formation of MYB–bHLH–WD40 Ternary Complexes

Liu

Hou

Jiang

et al. 2018

IJMS

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Flavan-3-ols and oligomeric proanthocyanidins (PAs) are the main nutritional polyphenols in green tea (Camellia sinensis), which provide numerous benefits to human health. To date, the regulatory mechanism of flavan-3-ol biosynthesis in green tea remains open to study. Herein, we report the characterization of a C. sinensis tryptophan-aspartic acid repeat protein (CsWD40) that interacts with myeloblastosis (MYB) and basic helix-loop-helix (bHLH) transcription factors (TFs) to regulate the biosynthesis of flavan-3-ols. Full length CsWD40 cDNA was cloned from leaves and was deduced to encode 342 amino acids. An in vitro yeast two-hybrid assay demonstrated that CsWD40 interacted with two bHLH TFs (CsGL3 and CsTT8) and two MYB TFs (CsAN2 and CsMYB5e). The overexpression of CsWD40 in Arabidopsis thaliana transparent testa glabra 1 (ttg1) restored normal trichome and seed coat development. Ectopic expression of CsWD40 alone in tobacco resulted in a significant increase in the anthocyanins of transgenic petals. CsWD40 was then coexpressed with CsMYB5e in tobacco plants to increase levels of both anthocyanins and PAs. Furthermore, gene expression analysis revealed that CsWD40 expression in tea plants could be induced by several abiotic stresses. Taken together, these data provide solid evidence that CsWD40 partners with bHLH and MYB TFs to form ternary WBM complexes to regulate anthocyanin, PA biosynthesis, and trichome development.

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“…Using gene ontology (GO) analysis, the DEPs were classified into three categories: cellular components (CC), molecular function (MF) and biological processes (BP) [19]. During the early phase of cold stress in both cultivars, the most highly represented categories were 'cell', 'cell part', 'intracellular', 'intracellular part' and 'cytoplasm' in CC (Fig.…”

Section: Primary Functional Classification Of Depsmentioning

confidence: 99%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

et al. 2020

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Background: Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results: The two kinds of commercial cultivars were exposed to a temperature of 0.5°C for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion: We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.

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Effects of vitro sucrose on quality components of tea plants (Camellia sinensis) based on transcriptomic and metabolic analysis

Cited by 37 publications

References 105 publications

Peroxidase, polyphenol oxidase activity and total phenolic concentration in birch (Betula pendula) in vitro shoots during rejuvenation

Peroxidase, polyphenol oxidase activity and total phenolic concentration in birch (Betula pendula) in vitro shoots during rejuvenation

A WD40 Repeat Protein from Camellia sinensis Regulates Anthocyanin and Proanthocyanidin Accumulation through the Formation of MYB–bHLH–WD40 Ternary Complexes

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

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