Floor mats are commonly used to improve the impact sound performance of existing homes, but actual impact sound reductions do not consistently appear, as they depend on the test-bed conditions used to determine impact sound performance. Therefore, this study evaluated the effects of the environmental conditions of the test bed on the impact sound reduction performances of different floor mats. The Korean industrial standard KS F 2865 specifies the measurement method in the test room and sets the thickness range of the available target floor structure to bare slabs with thicknesses ranging from 120 mm to 210 mm. The evaluation method is ∆L, which is the difference in standardized impact sound levels before and after the installation of the floor finishing material. In this study, a total of eight types of floor mats were tested in four different test beds according to KS F 2865. The impact sources used were tapping, bang machines, and a rubber ball, and we used these sources to consider both light-weight and heavy-weight impact sounds. The results were derived as the impact sound reduction performance for each frequency band and a single-number quantity. The results showed that light impact sound had a similar minimum reduction characteristic of at least 38 dB, regardless of the floor structure on which the mat was installed. However, the heavy-weight impact sound showed different tendencies depending on the floor mat and the characteristics of the floor structure of the test bed when a bang machine and a rubber ball were used. In particular, the reduction achieved by the bang machine showed less than half the tendency of the reduction by the rubber ball, and the tendency of the heavy-weight impact sound was shown to be reducible depending on how the floor mat was maintained, but the reductions differed depending on the test bed. The reductions were larger in the box-type test room than in the real-life-type test room, and among the box-type test rooms, the one with a thicker bare slab showed a relatively larger reduction. In addition, the reliability of the measurement results was evaluated through a correlation analysis between the single-number quantities depending on the thicknesses of the bare slabs of the test beds.