Effects of warming rates on physiological and molecular components of response to CTMax heat stress in the Antarctic fish Harpagifer antarcticus

Saravia, Julia; Paschke, Kurt; Oyarzún-Salazar, Ricardo; Cheng, C-H Christina; Navarro, Jorge M.; Vargas‐Chacoff, Luis

doi:10.1016/j.jtherbio.2021.103021

Cited by 23 publications

(11 citation statements)

References 76 publications

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“…9b). The optimal temperature value for the SIRT3-like deacetylase activity of the elephant shark could represent an evolutionary adjustment to the temperature regime of the habitat in which this species lives, as it has been demonstrated for other enzymes in other species (McCormick 1993;Somero 2004;Bilyk et al 2021;Saravia et al 2021). Thus, all subsequent enzymatic activity analyses for 𝚫NT-SIRT3-like were performed at 24ºC.…”

Section: Elephant Shark Sirt3-like Protein Has Deacetylase Activitymentioning

confidence: 99%

How many sirtuin genes are out there? evolution of sirtuin genes in vertebrates with a description of a new family member*

Opazo

Zavala

Vandewege

et al. 2020

Preprint

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Studying the evolutionary history of gene families is a challenging and exciting task with a wide range of implications. In addition to exploring fundamental questions about the origin and evolution of genes, disentangling their evolution is also critical to those who do functional/structural work, as the correct interpretation of their results needs to be done in a robust evolutionary context. The sirtuin gene family is a group of genes that are involved in a variety of biological functions mostly related to aging. Their duplicative history is an open question, as well as the definition of the repertoire of sirtuin genes among vertebrates. Our goal is to take advantage of the genomic data available in public databases to advance our understanding of how sirtuin genes are related to each other, and to characterize the gene repertoire in species representative of all the main groups of vertebrates. Our results show a well-resolved phylogeny that represents a significant improvement in our understanding of the duplicative history of the sirtuin gene family. We identified a new sirtuin family member (SIRT3-like) that was apparently lost in amniotes, but retained in all other groups of jawed vertebrates. Our results indicate that there are at least eight sirtuin paralogs among vertebrates and that all of them can be traced back to the last common ancestor of the group that existed between 676 and 615 millions of years ago.

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Section: Elephant Shark Sirt3-like Protein Has Deacetylase Activitymentioning

confidence: 99%

How many sirtuin genes are out there? evolution of sirtuin genes in vertebrates with a description of a new family member*

Opazo

Zavala

Vandewege

et al. 2020

Preprint

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“…9 b ). The optimal temperature value for the SIRT3.2 deacetylase activity of the elephant shark could represent an evolutionary adjustment to the temperature regime of the habitat in which this species lives, as it has been demonstrated for other enzymes in other species ( McCormick 1993 ; Somero 2004 ; Bilyk et al 2021 ; Saravia et al 2021 ). Thus, all subsequent enzymatic activity analyses for ΔNT-SIRT3.2 were performed at 24 °C.…”

Section: Resultsmentioning

confidence: 81%

How Many Sirtuin Genes Are Out There? Evolution of Sirtuin Genes in Vertebrates With a Description of a New Family Member

Opazo

Vandewege

Hoffmann

et al. 2023

Molecular Biology and Evolution

Self Cite

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Studying the evolutionary history of gene families is a challenging and exciting task with a wide range of implications. In addition to exploring fundamental questions about the origin and evolution of genes, disentangling their evolution is also critical to those who do functional/structural studies to allow a deeper and more precise interpretation of their results in an evolutionary context. The sirtuin gene family is a group of genes that are involved in a variety of biological functions mostly related to aging. Their duplicative history is an open question, as well as the definition of the repertoire of sirtuin genes among vertebrates. Our results show a well-resolved phylogeny that represents an improvement in our understanding of the duplicative history of the sirtuin gene family. We identified a new sirtuin gene family member (SIRT3.2) that was apparently lost in the last common ancestor of amniotes but retained in all other groups of jawed vertebrates. According to our experimental analyses, elephant shark SIRT3.2 protein is located in mitochondria, the overexpression of which leads to an increase in cellular levels of ATP. Moreover, in vitro analysis demonstrated it has deacetylase activity being modulated in a similar way to mammalian SIRT3. Our results indicate that there are at least eight sirtuin paralogs among vertebrates and that all of them can be traced back to the last common ancestor of the group that existed between 676 and 615 millions of years ago.

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“…Comparison among species that inhabit the same environments may differ in physiological strategies to cope with environmental thermal stress, constrained to a specific ecological scale (Peck, 2011;Byers, 2020). Therefore, physiological and genetic mechanisms are needed to understand the variation in thermal tolerance and distinguish phenotypic thermal strategy from adapting to extreme and cold Antarctic habitats, including physiological, molecular mechanisms (Gonzaĺez-Aravena et al, 2021;Saravia et al, 2021), and behavioral responses influenced changes in distribution ranges (Morley et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Upper thermal limits and risk of mortality of coastal Antarctic ectotherms

et al. 2023

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Antarctic marine animals face one of the most extreme thermal environments, characterized by a stable and narrow range of low seawater temperatures. At the same time, the Antarctic marine ecosystems are threatened by accelerated global warming. Determining the upper thermal limits (CTmax) is crucial to project the persistence and distribution areas of the Antarctic marine species. Using thermal death time curves (TDT), we estimated CTmax at different temporal scales from 1 minute to daily and seasonal, the predict vulnerability to the current thermal variation and two potential heatwave scenarios. Our results revealed that CTmax at 1 min are far from the temperature present in the marine intertidal area where our study species, showing Echinoderm species higher CTmax than the Chordata and Arthropods species. Simulations indicated that seasonal thermal variation from the intertidal zone contributed to basal mortality, which increased after considering moderate scenarios of heatwaves (+2°C) in the Shetland Archipelago intertidal zone. Our finding highlighted the relevance of including exposure time explicitly on the CTmax estimates, which deliver closer and more realistic parameters according to the species that may be experiencing in the field.

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Effects of warming rates on physiological and molecular components of response to CTMax heat stress in the Antarctic fish Harpagifer antarcticus

Cited by 23 publications

References 76 publications

How many sirtuin genes are out there? evolution of sirtuin genes in vertebrates with a description of a new family member*

How many sirtuin genes are out there? evolution of sirtuin genes in vertebrates with a description of a new family member*

How Many Sirtuin Genes Are Out There? Evolution of Sirtuin Genes in Vertebrates With a Description of a New Family Member

Upper thermal limits and risk of mortality of coastal Antarctic ectotherms

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