Effects of Water Deficit during Germination of Wheat Seeds

Miazek, A.; Bogdan, Janusz; Zagdańska, Barbara

doi:10.1023/a:1012455112853

Cited by 17 publications

(10 citation statements)

References 25 publications

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“…This is in agreement with the results of the effect of osmotic stress on the degradation of sugars in durum wheat, which revealed a drastic decrease of soluble sugars after 48 h of treatment (Almansouri et al, 2001). In contrast with our results, studies of germinating seeds of bread wheat showed an increase in soluble sugars started at day 2 of water deficit up to day 5 after 24 h of imbibition (Miazek et al, 2001).…”

Section: Discussioncontrasting

confidence: 99%

“…Seeds of bread wheat (Triticun aestivum L.) were imbibed for 24 hours, and then they were dehydrated for the subsequent days. The results showed a decrease in reserve mobilization started at day 5 of germination (Miazek et al, 2001). Indeed, germinating seeds of bread wheat showed tolerance to dehydration up to the 4P th P day post-imbibition (Miazek et al, 2001).…”

Section: Introductionmentioning

confidence: 91%

“…The results showed a decrease in reserve mobilization started at day 5 of germination (Miazek et al, 2001). Indeed, germinating seeds of bread wheat showed tolerance to dehydration up to the 4P th P day post-imbibition (Miazek et al, 2001). Whereas, in other studies, the effect of osmotic stress on the germination process of seeds revealed a highly significant decrease in the germination percentage, germination rate and reserve mobilization (Almansouri et al, 2001;Sayar et al, 2010;Soltani et al, 2006).…”

Section: Introductionmentioning

confidence: 92%

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Reserve Mobilization , Total Sugars and Proteins in Germinating Seeds of Durum Wheat ( Triticum Durum Desf. ) under Water Deficit after Short Period of Imbibition

Harb¹

2013

JJBS

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Imbibition during the first few hours is crucial for the success of seed germination process. The effect of water deficit after 2 and 8 hours (h) of imbibition of seeds of durum wheat was studied in terms of reserve mobilization, total sugars and proteins at 24, 48 and 72 h post treatment. Imbibition for 2 and 8 h was not enough to support normal mobilization of seed reserves and solubilization of sugars. But, a significant increase in total proteins was shown after 24 and 48 h of water deficit for the two imbibition periods (2 and 8 h). Our results indicate that most of the biochemical and molecular changes are intensified during the first 2-8 h of imbibition. Therefore, biochemical and molecular dissection of this phase of seed germination is of great value in the improvement of our understanding of plant's response to water deficit.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

Reserve Mobilization , Total Sugars and Proteins in Germinating Seeds of Durum Wheat ( Triticum Durum Desf. ) under Water Deficit after Short Period of Imbibition

Harb¹

2013

JJBS

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“…Half (52 %) of the 6-day-old seedlings survived. There is no direct relationship between water deficit and survival of wheat seedlings during post-germination growth (Miazek et al 2001). The 4-day-old seedlings were dehydration tolerant, but 6-day-old seedlings were dehydration sensitive (severe dehydration was a criterion for dehydration tolerance).…”

Section: Resultsmentioning

confidence: 96%

“…Wheat (Triticum aestivum L.) seedlings tolerate a high water deficit up to the 5th day of germination, but the appearance of the first leaf from the coleoptile coincided with a susceptibility to dehydration (Miazek et al 2001). One of the main reason of seedlings transition from tolerance to dehydration to sensitivity is supposed to be change of the hexose/sucrose ratio i.e.…”

Section: Introductionmentioning

confidence: 99%

Changes in the reduction state of ascorbate and glutathione, protein oxidation and hydrolysis leading to the development of dehydration intolerance in Triticum aestivum L. seedlings

2015

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The present work demonstrates that the transition of wheat (Triticum aestivum L.) seedlings from dehydration tolerance to intolerance on the fifth day following imbibition is associated with a disturbance in cellular redox homeostasis. During germination the ratio of reduced (AsA) to oxidized ascorbate (DHA) was lower in the tolerant 4-day-old seedlings compared to the sensitive 6-day-old seedlings because of the lower ascorbate content in the former. The reduced glutathione (GSH) and total glutathione (GSH ? GSSG) pools were higher in tolerant seedlings and remained higher upon dehydration. The development of dehydration intolerance with a seedling age coincided with a 50 % loss of the total glutathione pool and a shift of GSH/GSSG to a more oxidized state. Activities of ascorbate peroxidase and glutathione reductase increased with water deficiency in both tolerant and sensitive seedlings but the three new activity bands appeared only in sensitive seedlings. The stable ratio of GSH/GSSG and a higher AsA/DHA ratio in sensitive seedlings did not prevent the enhanced production of H2O2 and the peroxidation of lipids with dehydration. As a result, an increase in the protein carbonyl group and a significant decrease in the thiol groups were observed in dehydrated sensitive seedlings. Water deficiency enhanced the total azocaseinolytic activity, mainly in sensitive seedlings. The highest increase in protein carbonylation and lowest azocaseinolytic activity was observed at the beginning of seedling dehydration (15-25 % WSD) being in a sensitive phase of growth. The presented results indicate that the development of dehydration intolerance during the transition of wheat seedlings from heterotrophic to autotrophic growth is associated with an alterations in protein oxidation.

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Differential regulation of alanine aminotransferase homologues by abiotic stresses in wheat (Triticum aestivum L.) seedlings

2012

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Wheat (Triticum aestivum L.) seedlings contain four alanine aminotransferase (AlaAT) homologues. Two of them encode AlaAT enzymes, whereas two homologues act as glumate:glyoxylate aminotransferase (GGAT). To address the function of the distinct AlaAT homologues a comparative examination of the changes in transcript level together with the enzyme activity and alanine and glutamate content in wheat seedlings subjected to low oxygen availability, nitrogen and light deficiency has been studied. Shoots of wheat seedlings were more tolerant to hypoxia than the roots as judging on the basis of enzyme activity and transcript level. Hypoxia induced AlaAT1 earlier in roots than in shoots, while AlaAT2 and GGAT were unaffected. The increase in AlaAT activity lagged behind the increase in alanine content. Nitrogen deficiency has little effect on the activity of GGAT. In contrast, lower activity of AlaAT and the level of mRNA for AlaAT1 and AlaAT2 in wheat seedlings growing on a nitrogen-free medium seems to indicate that AlaAT is regulated by the availability of nitrogen. Both AlaAT and GGAT activities were present in etiolated wheat seedlings but their activity was half of that observed in light-grown seedlings. Exposure of etiolated seedlings to light caused an increase in enzyme activities and up-regulated GGAT1. It is proposed that hypoxia-induced AlaAT1 and light-induced peroxisomal GGAT1 appears to be crucial for the regulation of energy availability in plants grown under unfavourable environmental conditions.Key message In young wheat seedlings, both AlaAT and GGAT are down-regulated by nitrogen deficiency, whereas AlaAT1 is upregulated by hypoxia and GGAT1 by light.

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Effects of Water Deficit during Germination of Wheat Seeds

Cited by 17 publications

References 25 publications

Reserve Mobilization , Total Sugars and Proteins in Germinating Seeds of Durum Wheat ( Triticum Durum Desf. ) under Water Deficit after Short Period of Imbibition

Reserve Mobilization , Total Sugars and Proteins in Germinating Seeds of Durum Wheat ( Triticum Durum Desf. ) under Water Deficit after Short Period of Imbibition

Changes in the reduction state of ascorbate and glutathione, protein oxidation and hydrolysis leading to the development of dehydration intolerance in Triticum aestivum L. seedlings

Differential regulation of alanine aminotransferase homologues by abiotic stresses in wheat (Triticum aestivum L.) seedlings

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