Effects of Water Deficit on Proline Accumulation and Growth of two Cotton Genotypes of Different Drought Resistances

Ferreira, Luiz Gonzaga Rebouças; Souza, José Gomes de; Prisco, José Tarquı́nio

doi:10.1016/s0044-328x(79)80197-x

Cited by 30 publications

(21 citation statements)

References 29 publications

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“…Exogenously supplied proline can greatly facilitate cellular osmotic adjustment and growth in salt-containing medium (14). Similar results have been obtained in intact plants (8,17). These studies provided good evidence that the cellular basis for salt tolerance and the role of compatible solutes in salt stress found in whole plants could be investigated using suspension cultures.…”

supporting

confidence: 87%

“…A decline of protein synthesis, or extensive protein turnover, or increased synthesis of proline among other amino acids (28), could account for the accumulation of free amino acids, although we have not attempted to decide between these possibilities. In response to osmotic challenge, the synthesis and accumulation oflow-molecular compounds known as "compatible solutes" (proline, glycine-betaine, polyols, and quaternary amines) occur throughout the bacterial and plant kingdoms (5,8,17,24). It is hypothesized that these putative osmoprotectants may provide relief from the osmotic and/or salt stress (9, 12, 31).…”

Section: Discussionmentioning

confidence: 99%

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Influence of NaCl on Growth, Proline, and Phosphoenolpyruvate Carboxylase Levels in Mesembryanthemum crystallinum Suspension Cultures

Thomas¹,

Armond²,

Bohnert³

1992

Plant Physiol.

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The facultative halophyte Mesembryanthemum crystallinum responds to salt stress by increasing the levels of phosphoenolpyruvate carboxylase (PEPCase) and other enzymes associated with Crassulacean acid metabolism. A more common response to salt stress in sensitive and tolerant species, including M. crystallinum, is the accumulation of proline. We have established M. crystallinum suspension cultures to investigate whether both these salt-induced responses occur at the cellular level. Leafand root-derived cultures maintain 5% of the total soluble amino acids as proline. Cell culture growth slows upon addition of 400 millimolar NaCI, and proline levels increase to 40% of the total soluble amino acids. These results suggest a functional saltstress and response program in Mesembryanthemum cells. Suspension cultures grown with or without 400 millimolar NaCI have PEPCase levels that compare with those from roots and unstressed leaves. The predominant protein cross-reacting with an anti-PEPCase antibody corresponds to 105 kilodaltons (apparent molecular mass), whereas a second species of approximately 110 kilodaitons is present at low levels. In salt-stressed leaves, the 110 kilodalton protein is more prevalent. Levels of mRNA for both ppcl (salt stress induced in leaves) and ppc2 (constitutive) genes in sait-treated suspensions cultures are equal to unstressed leaves, and only twice the levels found in untreated suspension cultures. Whereas cells accumulate proline in response to NaCI, PEPCase protein amounts remain similar in salttreated and untreated cultures. The induction upon salt stress of the 110 kilodalton PEPCase protein and other Crassulacean acid metabolism enzymes in organized tissues is not observed in cell culture and may depend on tissue-dependent or photoautotrophydependent programs.

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supporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Influence of NaCl on Growth, Proline, and Phosphoenolpyruvate Carboxylase Levels in Mesembryanthemum crystallinum Suspension Cultures

Thomas¹,

Armond²,

Bohnert³

1992

Plant Physiol.

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“…Singh et al (26,27) Guerrier (16), Stewart and Lee (29) report positive correlations between proline accumulation and stress tolerance or adaptation to stress, the results of others such as Hanson et al (13), Tal et al. (33), and Ferreira et al (7) indicate a negative correlation between the two characteristics. One of the reasons for such a discrepancy could be that in some intact plants, the mechanism of tolerance may be other than or in addition to simple osmotic adjustment, e.g.…”

Section: Discussionmentioning

confidence: 94%

“…Addition of exogenous proline to the medium during water stress and during osmotic downshock alleviated the normally resulting inhibition of growth. The results suggest a positive role for proline accumulation in adaptation of cells to changing external water potentials.It has been suggested for some time that the often observed accumulation of proline by plant tissues during water stress is an adaptive response even though investigators have obtained varying results relating proline accumulation to enhanced survival or tolerance under stress (4,6,7,13,16,27,29). Although the relationship between proline levels and osmotic adjustment has rarely been determined, the most common interpretation of proline accumulation is that proline at high levels acts as a cytoplasmic osmotic solute.…”

mentioning

confidence: 99%

Proline Accumulation and the Adaptation of Cultured Plant Cells to Water Stress

Handa¹,

Handa²,

Hasegawa³

et al. 1986

Plant Physiol.

221

103

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The transfer of cultured tomato cells (Lycopersicon esculentum cv VFNT-Cherry) to a low water potential environment resulted in an increased dry weight to fresh weight ratio accompanied by a rapid accumulation of proline. Proline content continued to increase as osmotic adjustment and growth occurred. The initial increase in proline concentration was accompanied by a drop in turgor. However, proline levels continued to increase with a gain in turgor during osmotic adjustment. Thus, the accumulation of proline depended not only on cell water potential, or on the initial loss of turgor but more closely on cell osmotic potential. The ultimate level of proline depended on the level of adaptation. Proline levels remained high after more than 100 cell generations in low water potential media, but declined rapidly after transfer to media with a less negative water potential. Addition of exogenous proline to the medium during water stress and during osmotic downshock alleviated the normally resulting inhibition of growth. The results suggest a positive role for proline accumulation in adaptation of cells to changing external water potentials.It has been suggested for some time that the often observed accumulation of proline by plant tissues during water stress is an adaptive response even though investigators have obtained varying results relating proline accumulation to enhanced survival or tolerance under stress (4,6,7,13,16,27,29). Although the relationship between proline levels and osmotic adjustment has rarely been determined, the most common interpretation of proline accumulation is that proline at high levels acts as a cytoplasmic osmotic solute.The relationship between proline accumulation and osmotic adjustment of plant cells during a prolonged period of exposure to a constant low water potential (t)2 has not been determined before. Also, the water status parameter which is primarily associated with the initiation of the accumulation of proline in response to water stress has not been determined, i.e. whether it is the turgor (tp), osmotic potential (4's), or 4,6 of the cell. factors, such as stomatal physiology and morphology, plant and leaf size, root characteristics, etc. It is possible to circumvent such problems by using cultured plant cells where tolerance to water or salt stress must involve osmotic adjustment to prevent permanent loss of turgor and subsequent cell dehydration. Since cultured cells are in A equilibrium with the medium, it is possible to keep the cells at a constant over an extended period of time and eliminate the possibility that changes in growth which occur under imposed low external A are the result of an altered balance of water availability and loss.The objective of the present investigation was to determine the relationship between proline accumulation and the adaptation of cultured plant cells to water stress through osmotic adjustment. An attempt has been made also to assess which aspect of cell water relations is involved in the initiation of the accumulation of prolin...

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“…Este acumulo considerado como uma medida de tolerância ã seca (Levitt,I98O), também foi observado em feijão (Arcay & Rena, 1977;Sawazaki et al, 1981), arroz (Madruga & Rena, 1977); algodão (Ferreira et al . , 1979) (Raschke, 1975), como o acúmuIo de prolina (Aspinall & Paleg, 1981).…”

Section: Resultados ε Discussãounclassified

Relações entre o déficit hídrico e alguns processos fisiológicos e bioquímicos em quatro clones de seringueira.

1987

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RE SUMOWo ph-e^ente tAabalho ΖΔϊιιάοα-ΔΖ a vajviacao da h.eJ>-ù>tîncÂa dt{\U&iva eJ>tomattca, teoK de pn.oli.na tivne e clono^ila to tat datante am pexLodo de ctnqaenta dicu> αρά* α t>ub_ pen-sào da fiega em clone*, jovem de ae/iingueAAa, ÏAC 222, IAN 717, IAN S73 e IAM υ-c .oando-,&e avatiah t>uaj> ne&ibtencJjO& à t>eca. A toleXancAa ao deficit hldJvico detenmi nada atnavu da n.ej,l6tmcta di&u&iva e&tomcitica e pelo acumulo de ρη,οίληα tivne. Tambem avaltou-6C ο e^eito de de^-icAX hldnlco 6obne ο teoh. de clono^ila total. 0 clone IAN 873 apA-Zoantou o-b maJj> aJLtoi> vatohib da ne^-obtencMi eitomcuttca e de pnoLina IbjKe, enquanto ο teoh. de. cloho{ila dechtbcta com ο deficit hldhA.co, 0 clone IAN 7J7, companado ao& de moeù>, mo6tn.ua batxa heiiAtencia eAtomattca e ο menoh acumulo de photina lwhe,poh.çjm man tevc alto teoh de ctoho^tla duhante quae ζ Ζοάο ο pehlodo expeAÂjnental. 0& hÇAultadoé Δα gehem quo. ρκοίληα IMJH.IL e am pa/umetho majj, tndicadoh do nlvel de hidhai.ao.ao do tecido do que de heAtàtencta à ieca em i>ehÀ.ngueiAa. 0 clone IAN S73 ^o<. ο que ào^hcu ma-ioh. de htdhjxtacao. 0 clone 717 manteue oi> tecidoA maÂA hidhatadoA, Aendo pftovavelmente ο maÀA ne* intente.

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Effects of Water Deficit on Proline Accumulation and Growth of two Cotton Genotypes of Different Drought Resistances

Cited by 30 publications

References 29 publications

Influence of NaCl on Growth, Proline, and Phosphoenolpyruvate Carboxylase Levels in Mesembryanthemum crystallinum Suspension Cultures

Influence of NaCl on Growth, Proline, and Phosphoenolpyruvate Carboxylase Levels in Mesembryanthemum crystallinum Suspension Cultures

Proline Accumulation and the Adaptation of Cultured Plant Cells to Water Stress

Relações entre o déficit hídrico e alguns processos fisiológicos e bioquímicos em quatro clones de seringueira.

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