Abstract:The aim of the study is to estimate the effects of the water-holding capability of the polyvinyl formal (PVF) sponges on osteogenic response in vitro experiments. The rat bone marrow stem cells (BMCs) were seeded and cultured for up to 4 weeks under static conditions in osteogenic media to evaluate the adhesion, proliferation, differentiation, and mineralization on the Dextran-coated PVF sponges with or without water-holding capability. The BMCs seeded onto the PVF sponges with water-holding capability showed … Show more
“…The 3D printed polydopamine coated PLA composite showed cell adhesion, cell cycle progression, increased ALP activity, osteocalcin on culturing with hADSCs [137]. Dextran coated polyvinyl formal (PVF) sponges with water holding capacity showed more adhesion, proliferation, and differentiation of BMSCs in vitro along with increased DNA content, ALP activity, osteocalcin content, and calcium deposition [138].…”
Section: Surface Modification Of Scaffoldsmentioning
confidence: 99%
“…Osteoblasts adhesion, proliferation [136] PLA composite Polydopamine Normal cellular activity, osteogenic expression [137] PVF sponges Dextran Cell attachment, proliferation, osteogenic expression calcium deposition [138] [146].For example, the study with hBMSCs cultured on collagen/silk scaffolds in three different environments viz. static dish, spinner flask and perfusion system showed highest in vitro bone formation in perfusion system [147].…”
Section: Bioreactors For Bone Tissue Engineeringmentioning
confidence: 99%
“…Cells for bone tissue engineering BMSCs Osteoblast differentiation; osteoinduction; osteogenesis; mineralization; in vitro & in vivo bone regeneration[22,29,63,109,138,147,149,153] …”
Bone provides mechanical support, and flexibility to the body as a structural frame work along with mineral storage, homeostasis, and blood pH regulation. The repair and/or replacement of injured or defective bone with healthy bone or bone substitute is a critical problem in orthopedic treatment. Recent advances in tissue engineering have shown promising results in developing bone material capable of substituting the conventional autogenic or allogenic bone transplants. In the present review, we have discussed natural and synthetic scaffold materials such as metal and metal alloys, ceramics, polymers, etc. which are widely being used along with their cellular counterparts such as stem cells in bone tissue engineering with their pros and cons.
“…The 3D printed polydopamine coated PLA composite showed cell adhesion, cell cycle progression, increased ALP activity, osteocalcin on culturing with hADSCs [137]. Dextran coated polyvinyl formal (PVF) sponges with water holding capacity showed more adhesion, proliferation, and differentiation of BMSCs in vitro along with increased DNA content, ALP activity, osteocalcin content, and calcium deposition [138].…”
Section: Surface Modification Of Scaffoldsmentioning
confidence: 99%
“…Osteoblasts adhesion, proliferation [136] PLA composite Polydopamine Normal cellular activity, osteogenic expression [137] PVF sponges Dextran Cell attachment, proliferation, osteogenic expression calcium deposition [138] [146].For example, the study with hBMSCs cultured on collagen/silk scaffolds in three different environments viz. static dish, spinner flask and perfusion system showed highest in vitro bone formation in perfusion system [147].…”
Section: Bioreactors For Bone Tissue Engineeringmentioning
confidence: 99%
“…Cells for bone tissue engineering BMSCs Osteoblast differentiation; osteoinduction; osteogenesis; mineralization; in vitro & in vivo bone regeneration[22,29,63,109,138,147,149,153] …”
Bone provides mechanical support, and flexibility to the body as a structural frame work along with mineral storage, homeostasis, and blood pH regulation. The repair and/or replacement of injured or defective bone with healthy bone or bone substitute is a critical problem in orthopedic treatment. Recent advances in tissue engineering have shown promising results in developing bone material capable of substituting the conventional autogenic or allogenic bone transplants. In the present review, we have discussed natural and synthetic scaffold materials such as metal and metal alloys, ceramics, polymers, etc. which are widely being used along with their cellular counterparts such as stem cells in bone tissue engineering with their pros and cons.
“…The non-biodegradable capacity of PVF films encouraged to use them in biomedical engineering for longstanding performance. However due to low levels of biocompatibility, the PVF films could only be used with special coatings [2]- [4]. For example, the co-polymerization of PVF films with the grafting of N-vinylpyrrolidone demonstrated excellent resistance of bacterial adhesion of S. aureus, E. coli and P. aeruginosa [2].…”
In this study, surface modification of fluoropolymer Polyvinyl fluoride (PVF) films was performed using Extreme Ultraviolet (EUV) radiations to induce patterned structures on surface and to provide control over the surface wettability. Specially developed laser produced plasma based EUV source was used for surface structuring. The double stream gas-puff target was produced by injection of krypton and xenon (KrXe) gas mixture into a hollow stream of helium. Commercially available EKSPLA Nd:YAG 1.06 micron laser was used to irradiate the KrXe gas puff target with 3 nanosecond pulse duration having 0.8 J energy. The PVF films were irradiated with 50 and 200 EUV pulses. The surface characterisation of the pristine and EUV modified PVF polymer films was performed by Atomic Force Microscopy (AFM) for morphological modifications. To investigate chemical modifications, X-ray Photoelectron Spectroscopy was used. The wettability of the sample surfaces was examined by Water Contact Angle (WCA) measurements. EUV surface modification of PVF films resulted in formation of wall type rippled structures on the polymer surfaces. The surface roughness of the EUV treated surfaces was increased up to 287 nm and 21° reduction was observed in the WCA of the PVF films. Successful surface structuring and wettability control was obtained using EUV surface modification of PVF films.
“…The dextran 40 in LR-3T-5D inhibits the sedimentation of cells in bags or syringes and helps maintain a given concentration of cells at the time of administration (Fujita et al 2020 ). However, dextran coatings reportedly enhance the adhesion of BM-MSCs to sponge scaffolds (Togami et al 2014 ); thus, the adhesion-enhancing effect of dextran 40 may be responsible for the observed high degree of cell aggregation in LR-3T-5D at room temperature. Figure 2.…”
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
