Effects of water quality on in vitro fertilization and development of bovine oocytes in protein-free medium

Nagao, Yoshikazu; Saeki, Kazuhiro; Hoshi, Masaki; Takahashi, Yoshiyuki; Kanagawa, Hiroshi

doi:10.1016/0093-691x(95)00197-g

Cited by 31 publications

(29 citation statements)

References 18 publications

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“…The application of mineral oil significantly reduced embryo development compared with the presence of paraffin oil although these two types of oil have been broadly used in embryo culture [2,10,11,37]. A recent study indicates that the use of mineral oil significantly reduces the rate of pig embryos developing to the blastocyst stage after IVF [38].…”

Section: Discussionmentioning

confidence: 99%

Optimisation of in vitro culture conditions in B6CBF1 mouse embryos

2004

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-This study was undertaken to investigate the effects of three media, volume and type of oil and frequency of observation on the in vitro development of mouse zygotes. B6CBF1 female mice (4 to 6 wk old) were superovulated using PMSG/hCG and mated with a proven fertile male of the same strain. Putative zygotes with polar bodies were collected from the oviducts of mated mice, 25-28 h after hCG injection, and were cultured in vitro. Embryo development was evaluated at either 96 h and 120 h or every 24 h for 120 h. The results obtained showed that the CZB medium was better than the KSOM and HCO 3 HTF media, and the use of 1 mL of paraffin oil was better than the use of 0.5 mL of paraffin oil. The effect of paraffin oil and mineral oil on embryo development was examined and the results indicated that the use of paraffin oil was better than the use of mineral oil. Repeated observations did not influence the proportion of embryos developing to blastocysts.in vitro development / media / type of oil / frequency of observation / mouse embryos

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Section: Discussionmentioning

confidence: 99%

Optimisation of in vitro culture conditions in B6CBF1 mouse embryos

2004

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“…Water quality and storage period seriously affect bovine embryo development in protein-free medium [22]. In our laboratory, pre-treated water obtained by reverse osmosis and electrodeionization from an Elix system is deionized, ultrafiltered (5 KDa), UVA-rays treated and sterile filtered (0.22 µm) through a Milli-Q system (Gradient A-10).…”

Section: Discussionmentioning

confidence: 99%

“…The protective effect of BSA could be more important than its nutritive role [21]. Water quality used in bovine embryo culture media strongly affects embryo development [22], and variations in embryo development and pregnancy rates achieved among in vitro fertilization laboratories may be partially attributable to differences in the water quality used to prepare culture media [23]. In vitro fertilization (IVF) laboratories can obtain water for their cultures from tap water purification or commercially available water (tested for cell or embryo culture).…”

Section: Introductionmentioning

confidence: 99%

Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and quality

et al. 2003

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-This study evaluated the protective effect of protein, as dependent on osmolarity, and the quality of water sources used to prepare embryo culture media. In Experiment 1, two concentrations of NaCl were used to obtain culture media with normal (280 mOSM) and low (245 mOSM) osmolarity, each supplemented with either bovine serum albumin (BSA) or polyvinyl alcohol (PVA). Low osmolarity improved blastocyst rates in the presence of BSA (P < 0.01) and tended to do it in medium containing PVA (P < 0.07). Furthermore, low osmolarity allowed PVA to increase inner cell mass (ICM) numbers and ICM/total cell rate (P < 0.05), while trophectoderm (TE) and total cell counts tended to decrease (P < 0.08). In Experiment 2, culture media were prepared with two water sources (Milli-Q and Sigma-W3500-) in combination with BSA or PVA. Both water sources yielded similar embryo development rates, but in the presence of BSA, Milli-Q water produced embryos with increased ICM/total cells rates (P < 0.05). On the contrary, Sigma water tended to increase trophectoderm cell counts (P < 0.08). In conclusion, the present study showed that low osmolarity is beneficial to embryo development and combinations of macromolecule and osmolarity influence trophectoderm differentiation. Both Milli-Q and Sigma supported embryo development at comparable rates, although in the presence of BSA, blastocysts obtained in the medium prepared with Milli-Q water had superior quality in terms of ICM/total cells rates.in vitro produced bovine embryo / osmolarity / water quality / protein / cell number

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“…Following incubation, some oocytes were fixed in acetic alcohol (1:3) and stained with aceto-orcein for examination of the nuclear stage [18]. The COCs were washed five times thoroughly and cultured in m-TCM199 supplemented with 0.02 AU/ml FSH and 1 µg/ml E 2 (maturation medium) [16] in a plastic dish covered with mineral oil (10 COCs/50 µl droplet) for 12-24 hr at 39°C in 5% CO 2 in air at high humidity.In vitro fertilization was carried out as described by Nagao et al [11]. Briefly, COCs cultured for 16, 20 and 24 hr in the maturation medium were inseminated with Percollwashed spermatozoa [16] (1 × 10 6 cells/ml) in defined medium [1] modified by excluding glucose and supplemented with 1 µg/ml heparin [14] (10 COCs/100 µl droplet).…”

mentioning

confidence: 99%

“…In vitro fertilization was carried out as described by Nagao et al [11]. Briefly, COCs cultured for 16, 20 and 24 hr in the maturation medium were inseminated with Percollwashed spermatozoa [16] (1 × 10 6 cells/ml) in defined medium [1] modified by excluding glucose and supplemented with 1 µg/ml heparin [14] (10 COCs/100 µl droplet).…”

mentioning

confidence: 99%

Timing of Completion of the First Meiotic Division in Bovine Oocytes after Maintenance of Meiotic Arrest with Cycloheximide and Their Subsequent Development.

Saeki

Nagao

Kishi

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. This study examined the timing of completion of meiosis I of bovine oocytes in which meiotic resumption had been inhibited by cycloheximide (CHX), and also determined the optimum interval of maturation in culture for subsequent fertilization and development. Most oocytes treated with CHX reached metaphase II at 16 hr in the maturation culture, while control oocytes did at 20 hr. CHX-treated oocytes cultured for 16 hr were normally fertilized but failed to develop into blastocysts. Maturation in culture for 20 hr resulted in comparable development for control oocytes. The results indicate that nuclear maturation of CHX-treated oocytes was completed 4 hr faster than for control oocytes, however the same interval of maturation as that of control oocytes is necessary for subsequent development to blastocysts. . Ten COCs were incubated in 50 µl drops of m-TCM199 supplemented with 10% (v/v) fetal calf serum (Gibco, m-TCM-199+FCS) and 10 µg/ml cycloheximide (CHX, Sigma), 0.02 AU/ml FSH (Antoine, Denka Seiyaku, Tokyo, Japan) and 1 µg/ml estradiol-17β (E 2 , Sigma) in a plastic dish (Falcon 1007, Becton and Dickinson Labware, Lincoln Park, NJ, U.S.A.) covered with mineral oil (Squibb, Princeton, NJ, U.S.A.) for 24 hr at 39°C in 5% CO 2 in air at high humidity. Following incubation, some oocytes were fixed in acetic alcohol (1:3) and stained with aceto-orcein for examination of the nuclear stage [18]. The COCs were washed five times thoroughly and cultured in m-TCM199 supplemented with 0.02 AU/ml FSH and 1 µg/ml E 2 (maturation medium) [16] in a plastic dish covered with mineral oil (10 COCs/50 µl droplet) for 12-24 hr at 39°C in 5% CO 2 in air at high humidity.In vitro fertilization was carried out as described by Nagao et al. [11]. Briefly, COCs cultured for 16, 20 and 24 hr in the maturation medium were inseminated with Percollwashed spermatozoa [16] (1 × 10 6 cells/ml) in defined medium [1] modified by excluding glucose and supplemented with 1 µg/ml heparin [14] (10 COCs/100 µl droplet). Oocytes and spermatozoa were co-incubated for 6 hr at 39°C in 5% CO 2 in air with high humidity. Following co-incubation, the oocytes were freed from the spermatozoa and cumulus cells and cultured in modified synthetic oviduct fluid medium (m-SOFM) [20] supplemented with 3 mg/ml bovine serum albumin (A-4378, Sigma) for 7 days (168 hr) at 39°C in 5% CO 2 , 5% O 2 and 90% N 2 with high humidity (20-30 embryos/50 µl droplet) [12]. Some embryos were fixed and stained 18 hr after insemination for assessment of fertilization [16]. Total fertilization included all penetrated Mammalian extrafollicular oocytes resume meiosis spontaneously [15]. Active protein synthesis is required for the resumption in oocytes of pigs, sheep, goats and cattle [5,6,8,9]. Bovine oocytes do not undergo germinal vesicle breakdown (GVBD) in media with protein synthesis inhibitors such as cycloheximide (CHX) [6,19,21] and puromycin [10]. The inhibition of GVBD is fully reversible, since after washing the inhibitors, oocytes undergo GVBD [6,10,21]. Moreover...

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Effects of water quality on in vitro fertilization and development of bovine oocytes in protein-free medium

Cited by 31 publications

References 18 publications

Optimisation of in vitro culture conditions in B6CBF1 mouse embryos

Optimisation of in vitro culture conditions in B6CBF1 mouse embryos

Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and quality

Timing of Completion of the First Meiotic Division in Bovine Oocytes after Maintenance of Meiotic Arrest with Cycloheximide and Their Subsequent Development.

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