Effects of water-soluble components of atmospheric particulates from rare earth mining areas in China on lung cancer cell cycle

Yuan, Xia; Zhang, Xulong; Sun, Dejun; Gao, Yumin; Zhang, Xiaoe; Wang, Li; Cai, Qingjun; Wang, Qihao; Sun, Juan

doi:10.1186/s12989-021-00416-z

Cited by 7 publications

(4 citation statements)

References 44 publications

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“…These finding indicates that RPL18A is lynchpin for the radioprotective effect of MLT. Studies have shown that inhibition of RPL18A expression leads to cell cycle arrest in lung cancer 43 and inhibits the proliferation of mesothelioma cells 44 . In addition, RPL18A could serve as a potential biomarker for clear cell renal cell carcinoma 45 .…”

Section: Discussionmentioning

confidence: 99%

Amelioration of gamma irradiation‐induced salivary gland damage in mice using melatonin

Ren

Zhu

Wang

et al. 2023

Journal of Pineal Research

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Salivary gland damage caused by ionizing radiation (IR) severely affects the patient quality of life and influences the efficacy of radiotherapy. Most current treatment modalities are palliative, so effective prevention of damage caused by IR is essential. Melatonin (MLT) is an antioxidant that has been reported to prevent IR‐induced damage in a range of systems, including the hematopoietic system and gastrointestinal tract. In this study, we explored the effects of MLT on whole‐neck irradiation (WNI)‐induced salivary gland damage in mice. The results revealed that by protecting the channel protein AQP‐5, MLT not only alleviates salivary gland dysfunction and maintains salivary flow rate, but also protects salivary gland structure and inhibits the WNI‐induced reduction in mucin production and degree of fibrosis. Compared with WNI‐treated mice, in those receiving MLT, we observed a modulation of oxidative stress in salivary glands via its effects on 8‐OHdG and SOD2, as well as an inhibition of DNA damage and apoptosis. With respect to its radioprotective mechanism, we found that MLT may alleviate WNI‐induced xerostomia partly by regulating RPL18A. In vitro, we demonstrated that MLT has radioprotective effects on salivary gland stem cells (SGSCs). In conclusion, our data this study indicate that MLT can effectively alleviate radiation‐induced damage in salivary glands, thereby providing a new candidate for the prevention of WNI‐induced xerostomia.

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Section: Discussionmentioning

confidence: 99%

Amelioration of gamma irradiation‐induced salivary gland damage in mice using melatonin

Ren

Zhu

Wang

et al. 2023

Journal of Pineal Research

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“…It has been reported that RP deficiency alters the expression of eight major functional genes, including the cell cycle, cell metabolism, signal transduction, and development [21]. For example, inhibition of RPL13 expression by siRNA in A549 cells can block the cell cycle in the G1 phase, thereby influencing the expression level of CDK2, Cyclin D1, E2F1, p21, and RB1 [22]. When RPL32 is silenced, RPL5 and RPL11 translocate from the nucleolus to the nucleoplasm through ribosomal stress, where they bind to murine double minute 2 and block it from binding to Fig.…”

Section: Discussionmentioning

confidence: 99%

Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease

Li,

Zhang,

Dong

et al. 2023

J Transl Med

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Background Although chronic inflammation, oxidative stress, airway remodeling, and protease-antiprotease imbalance have been implicated in chronic obstructive pulmonary disease (COPD), the exact pathogenesis is still obscure. Gene transcription and post-transcriptional regulation have been taken into account as key regulators of COPD occurrence and development. Identifying the hub genes and constructing biological regulatory networks at the post-transcriptional level will help extend current knowledge on COPD pathogenesis and develop potential drugs. Methods All lung tissues from non-smokers (n = 6), smokers without COPD (smokers, n = 7), and smokers with COPD (COPD, n = 7) were collected to detect messenger RNA (mRNA), microRNA (miRNA), circular RNA (circRNA), and long non-coding RNA (lncRNA) expression and identify the hub genes. Biological regulatory networks were constructed at the post-transcriptional level, including the RNA-binding protein (RBP)-hub gene interaction network and the competitive endogenous RNA (ceRNA) network. In addition, we assessed the composition and abundance of immune cells in COPD lung tissue and predicted potential therapeutic drugs for COPD. Finally, the hub genes were confirmed at both the RNA and protein levels. Results Among the 20 participants, a total of 121169 mRNA transcripts, 1871 miRNA transcripts, 4244 circRNA transcripts, and 122130 lncRNA transcripts were detected. There were differences in the expression of 1561 mRNAs, 48 miRNAs, 33 circRNAs, and 545 lncRNAs between smokers and non-smokers, as well as 1289 mRNAs, 69 miRNAs, 32 circRNAs, and 433 lncRNAs between smokers and COPD patients. 18 hub genes were identified in COPD. TGF-β signaling and Wnt/β-catenin signaling may be involved in the development of COPD. Furthermore, the circRNA/lncRNA-miRNA-mRNA ceRNA networks and the RBP-hub gene interaction network were also constructed. Analysis of the immune cell infiltration level revealed that M2 macrophages and activated NK cells were increased in COPD lung tissues. Finally, we identified that the ITK inhibitor and oxybutynin chloride may be effective in treating COPD. Conclusions We identified several novel hub genes involved in COPD pathogenesis. TGF-β signaling and Wnt/β-catenin signaling were the most dysregulated pathways in COPD patients. Our study constructed post-transcriptional biological regulatory networks and predicted small-molecule drugs for the treatment of COPD, which enhanced the existing understanding of COPD pathogenesis and suggested an innovative direction for the therapeutic intervention of the disease.

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“…Endometriosis is a benign disease, but it has the ability to invade and maintain the survival function of ectopic tissue, similar to cancer. RPL18A is involved in the cycle arrest process of non-small cell lung cancer A549 cells by affecting the expression of several cell cycle-related proteins, including Cyclin A2 and Cyclin B1 ( 34 ). The expression of GNB2L1 was higher in ectopic endometrial tissue from patients with endometriosis than in normal patients, regardless of whether they were in the proliferative or secretory phase of the menstrual cycle.…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Molecular Mechanism Related to Infertile Endometriosis

Guo

Zhang

et al. 2022

Front. Vet. Sci.

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ObjectivesIn this research, we aim to explore the bioinformatic mechanism of infertile endometriosis in order to identify new treatment targets and molecular mechanism.MethodsThe Gene Expression Omnibus (GEO) database was used to download MRNA sequencing data from infertile endometriosis patients. The “limma” package in R software was used to find differentially expressed genes (DEGs). Weighted gene co-expression network analysis (WGCNA) was used to classify genes into modules, further obtained the correlation coefficient between the modules and infertility endometriosis. The intersection genes of the most disease-related modular genes and DEGs are called gene set 1. To clarify the molecular mechanisms and potential therapeutic targets for infertile endometriosis, we used Gene Ontology (GO), Kyoto Gene and Genome Encyclopedia (KEGG) enrichment, Protein-Protein Interaction (PPI) networks, and Gene Set Enrichment Analysis (GSEA) on these intersecting genes. We identified lncRNAs and miRNAs linked with infertility and created competing endogenous RNAs (ceRNA) regulation networks using the Human MicroRNA Disease Database (HMDD), mirTarBase database, and LncRNA Disease database.ResultsFirstly, WGCNA enrichment analysis was used to examine the infertile endometriosis dataset GSE120103, and we discovered that the Meorangered1 module was the most significantly related with infertile endometriosis. The intersection genes were mostly enriched in the metabolism of different amino acids, the cGMP-PKG signaling pathway, and the cAMP signaling pathway according to KEGG enrichment analysis. The Meorangered1 module genes and DEGs were then subjected to bioinformatic analysis. The hub genes in the PPI network were performed KEGG enrichment analysis, and the results were consistent with the intersection gene analysis. Finally, we used the database to identify 13 miRNAs and two lncRNAs linked to infertility in order to create the ceRNA regulatory network linked to infertile endometriosis.ConclusionIn this study, we used a bioinformatics approach for the first time to identify amino acid metabolism as a possible major cause of infertility in patients with endometriosis and to provide potential targets for the diagnosis and treatment of these patients.

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Effects of water-soluble components of atmospheric particulates from rare earth mining areas in China on lung cancer cell cycle

Cited by 7 publications

References 44 publications

Amelioration of gamma irradiation‐induced salivary gland damage in mice using melatonin

Amelioration of gamma irradiation‐induced salivary gland damage in mice using melatonin

Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease

Identification of Potential Molecular Mechanism Related to Infertile Endometriosis

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