Effects of Water Stress on Sorbitol Production and Anatomical Changes in the Nuclei of Leaf and Root Cells of Young Loquat Trees

Cui, Shi-mao; Chen, Gui Lin; Nii, Naosuke

doi:10.2503/jjshs.72.359

Cited by 14 publications

(7 citation statements)

References 13 publications

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“…Sorbitol accumulation in apple leaves may also represent a good biochemical marker of moderate drought stress and a useful parameter for developing precise irrigation schedules in apple orchards [22]. Water stress also induces sorbitol accumulation in loquat leaves [23]. Sorbitol or other polyols may function as compatible solutes in transgenic tobacco [24], Plantago spp.…”

Section: Introductionmentioning

confidence: 99%

Water Deficit Affects the Growth and Leaf Metabolite Composition of Young Loquat Plants

Gugliuzza

Talluto

Martinelli

et al. 2020

Plants

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Water scarcity in the Mediterranean area is very common and understanding responses to drought is important for loquat management and production. The objective of this study was to evaluate the effect of drought on the growth and metabolism of loquat. Ninety two-year-old plants of ‘Marchetto’ loquat grafted on quince were grown in the greenhouse in 12-liter pots and three irrigation regimes were imposed starting on 11 May and lasting until 27 July, 2013. One-third of the plants was irrigated with 100% of the water consumed (well watered, WW), a second group of plants was irrigated with 66% of the water supplied to the WW plants (mild drought, MD), and a third group was irrigated with 33% of the water supplied to the WW plants (severe drought, SD). Minimum water potential levels of −2.0 MPa were recorded in SD plants at the end of May. Photosynthetic rates were reduced according to water supply (WW > MD > SD), especially during the morning hours. By the end of the trial, severe drought reduced all growth parameters and particularly leaf growth. Drought induced early accumulation of sorbitol in leaves, whereas other carbohydrates were not affected. Of over 100 leaf metabolites investigated, 9 (squalene, pelargonic acid, glucose-1-phosphate, palatinol, capric acid, aconitic acid, xylitol, lauric acid, and alanine) were found to be useful to discriminate between the three irrigation groups, suggesting their involvement in loquat metabolism under drought conditions. Loquat behaved as a moderately drought-tolerant species (limited stem water potential and growth reductions) and the accumulation of sorbitol in favor of sucrose in mildly-stressed plants may be considered an early protective mechanism against leaf dehydration and a potential biochemical marker for precise irrigation management.

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Section: Introductionmentioning

confidence: 99%

Water Deficit Affects the Growth and Leaf Metabolite Composition of Young Loquat Plants

Gugliuzza

Talluto

Martinelli

et al. 2020

Plants

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“…All plants were transferred into a greenhouse to induce a rapid and drastic water stress. The degree of drought stress was judged from leaf orientation and color, as described by Cui et al (2003). When plants reached the level of severe stress, they were watered from the bottom of the container.…”

Section: Methodsmentioning

confidence: 99%

“…Although a review revealed that many compounds accumulate under stress conditions, only a few reports have described physiologically and anatomically why Rosaceae trees can tolerate drought conditions (Cui et al, 2003. Knowledge of anatomical changes in the roots should contribute to our understanding of drought tolerance in Rosaceae trees.…”

Section: Introductionmentioning

confidence: 99%

Anatomical Development of Phi Thickening and the Casparian Strip in Loquat Roots

Pan¹,

Nakao²,

Nii³

2006

J. Japan. Soc. Hort. Sci.

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“…Osmotic stress promotes sorbitol accumulation in the leaves and roots of loquat (Eriobotrya japonica) trees, and sorbitol content diminishes gradually once the osmotic stress is alleviated (Cui et al 2003). Low temperature and drought stress also led to an increased concentration of sorbitol in peach leaves (Deguchi et al 2002;Cui et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Three Sorbitol Transporter Genes in Micropropagated Apple Plants Grown under Drought Stress

Lei

Zhao

et al. 2011

Plant Mol Biol Rep

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Sorbitol, a major end-product of photosynthesis in many species of the Rosaceae family, accumulates in response to abiotic stressors. However, the relationship that arises between the expression of sorbitol transporters and sorbitol accumulation under abiotic stress remains unclear. In this study, micropropagated 'Fuji' apple plants (Malus domestica Borkh. 'Fuji') were exposed to two varying degrees of osmotic stress and compared relative to an unstressed control. The osmotic stress was generated by adding PEG 6000 into full-strength Hoagland solution and adjusted the osmotic potential to either −0.75 MPa (mild drought stress [MIS]) or −1.5 MPa (severe drought stress [SES]). Analysis of sorbitol levels via high performance liquid chromatography (HPLC) showed that the sorbitol concentration was elevated in roots, phloem tissues and leaves in both the MIS and SES treatments compared to controls for the entire duration of the experiment. Three cDNA sequences, encoding sorbitol transporters (MdSOT3, MdSOT4 and MdSOT5), were isolated from leaves. Realtime quantitative PCR (RT-qPCR) data suggests that the expression levels of MdSOT3 and MdSOT5 were higher under MIS and SES in roots, phloem tissues and leaves compared to unstressed controls. The average mRNA levels of MdSOT4 in phloem tissues declined under both drought treatments (with the exception being at 2 h of SES). In roots and leaves under SES, mRNA production was increased. These results indicate that the up-regulation of MdSOT3 and MdSOT5 expression is consistent with the accumulation of sorbitol under conditions of osmotic stress in apple plants. They enhanced drought tolerance in vegetative tissues. Increased MdSOT4 mRNA enhanced drought tolerance under SES.

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Effects of Water Stress on Sorbitol Production and Anatomical Changes in the Nuclei of Leaf and Root Cells of Young Loquat Trees

Cited by 14 publications

References 13 publications

Water Deficit Affects the Growth and Leaf Metabolite Composition of Young Loquat Plants

Water Deficit Affects the Growth and Leaf Metabolite Composition of Young Loquat Plants

Anatomical Development of Phi Thickening and the Casparian Strip in Loquat Roots

Characterization of Three Sorbitol Transporter Genes in Micropropagated Apple Plants Grown under Drought Stress

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