Effects of whole blood flow rates on mononuclear cell yields during peripheral blood stem cell collection using fenwal CS 3000 plus

Abstract

It is generally assumed that using high whole blood flow rates (WBFR), 80 ml/min, during peripheral blood stem cell (PBSC) collection on the Fenwal CS 3000 Plus blood cell processor will result in higher yields of mononuclear cells (MNC) than using lower WBFR (50 ml/min). To test this assumption, we retrospectively studied 129 PBSC procedures on 17 patients in a multiple myeloma protocol comparing MNC yield, as well as red blood cell (RBC), granulocyte, and platelet (Plt) content, of four average WBFR groups. Standard PBSC procedures were performed using modified procedure 1, interface offset 100, anticoagulant (AC) ratio of 11:1, small volume collection chamber, and a processing time of 4 hours. After correcting for AC volume used, the volume processed was divided by 240 minutes to obtain the average WBFR. WBFRs were separated into 4 groups of 40-49, 50-59, 60-69, and 70-79 ml/min. When compared to the highest flow rate group (70-79 ml/min), the three lower flow rate groups had significantly higher MNC yields of 16.2 +/- 6.9, 13.1 +/- 5.1, and 11.5 +/- 4.7 x 10(9), respectively, as compared to 8.9 +/- 6.1 x 10(9) MNC for the 70-79 ml/min group. There was no significant difference in granulocyte yield which ranged from 1.6 +/- 2.1 to 4.5 +/- 4.8 x 10(9).(ABSTRACT TRUNCATED AT 250 WORDS)

Apheresis instrument settings influence infused absolute lymphocyte count affecting survival following autologous peripheral hematopoietic stem cell transplantation in non-Hodgkin's lymphoma: the need to optimize instrument setting and define a lymphocyte collection target

“…Previous work showed that the slower flow rates actually yielded more MNC than high flow rates, likely owing to more dwell time in the centrifuge. 17 The smaller amount of whole blood processed probably explains the lower numbers of lymphocytes in CS vs SP collections. The CS was the machine of choice for the first 4 years of the study and then was replaced by SP and AM owing to higher CD34-positive cell yields and pending obsolescence of the CS by Baxter.…”

Cobe Spectra is superior to Fenwal CS 3000 Plus for collection of hematopoietic stem cells

“…Lin et al 13 showed that increasing the flow rate beyond the recommended 50 ml/min decreased CE for mononuclear cells and the total number of mononuclear cells collected with CS 3000 Plus. Our procedures were performed at 85 ml/min -a rate in excess of that recommended by the manufacturer.…”

“…7 A number of studies have compared CEs of different cell separators retrospectively and prospectively. [8][9][10][11][12][13] The practice at our institution has been to use CS 3000 Plus cell separators almost exclusively for leukapheresis until recently when Cobe Spectra separators began to be employed regularly. This retrospective analysis was undertaken to compare the CEs of the two machines to see if there was a difference between them -and to determine the best way of employing different cell separators under normal clinical circumstances.…”

CD34+ cell collection efficiency does not correlate with the pre-leukapheresis hematocrit

“…In the case of the Fenwal CS 3000 Plus machine, changing the blood flow rate affects the CE. 12 Lin et al 12 showed that increasing the flow rate beyond the recommended 50 ml/min decreased CE for mononuclear cells and the total number of mononuclear cells collected. It is noteworthy that our procedures were performed at 85 ml/min -a rate far in excess of what is recommended by the manufacturer -which allowed 15 l blood to be processed in 3 h. This probably accounts for the wide range and relatively low median CE seen in this study.…”

“…8 Technical issues surrounding the clinical aspects of leukapheresis have been studied less extensively. [9][10][11][12][13][14][15][16][17][18][19][20][21][22] Most of these studies have compared different cell separators or collection techniques. Data on collection efficiency (CE) of cell separators, especially with reference to the collection of CD34 ϩ cells, are limited.…”