Background: A decline in neuromuscular function can cause a decrease in physical fitness in elderly women. Objective: The present study aimed at investigating whether whole body vibration (WBV) training and creatine supplementation could affect muscle strength, mobility, and balance in elderly women in short-term. Methods: The participants were 22 healthy elderly women aged 60 to 80 years, who were randomly divided into the whole-body vibration and creatine (WBV + Cr) group, whole-body vibration and placebo (WBV + P) group, and control group. The whole-body vibration group performed exercises for 10 days. The participants in WBV + Cr group consumed 20 g oral creatine supplement per day from the first to the fifth day, which was followed by consuming 5 g of creatine per day for the next 5 days. To assess mobility performance, a 30-meter walking and tandem gait tests were applied. Static and dynamic balance were measured by Flamingo and Timed-Up and Go (TUG) tests. Muscle isometric and isotonic strength were assessed by dynamometer and leg extension tests. Results: No significant difference was found in static balance (P = 0.11), but WBV + Cr and WBV + P groups showed better results in dynamic balance (P = 0.001 and P = 0.009, respectively). Moreover, leg isotonic strength improved significantly in WBV + Cr and WBV + P groups (P = 0.001 and P = 0.001, respectively). However, leg isometric strength and 30-meter walking performance significantly improved only in WBV + Cr group compared to the control group. Moreover, no significant differences were observed in isometric strength of the hand (P = 0.89) and tandem gait test results among the groups (P = 0.25). Conclusions: A short-term WBV exercise improves isotonic strength of the leg muscle and dynamic balance, while creatine supplementation with and without WBV training increases isometric strength of the leg muscle and mobility in elderly women.