Thermal comfort is one of the most important components of comfort which shows physiological, psychological, and physical harmony between human body and environment. The heat and moisture transfer capacity of fabric from skin to environment affects the thermal comfort of garments. The transfer capacity depends on the characteristic features of raw materials and fabric structural properties. In this study, it is aimed to determine the advantages of knitted fabric types, taking into account the environmental condition and activity level by measuring the thermal comfort properties such as air permeability, wicking, moisture management, thermal and water vapor resistances. Two knitted structures composed of tuck and float combinations and six raw materials were chosen for the fabrics produced. According to the measurement results, the polyester and cotton/Coolmax fabrics with float stitches have had good liquid moisture transport properties. Besides, high air permeability and low water vapor resistance have been obtained in viscose and Tencel LF fabrics with tuck stitches. On the basis of the results obtained in this investigation, and taking into consideration thermal behavior of human body, four women's and five men's T-shirts are designed. These T-shirt designs may be helpful for further approaches on the optimization of thermal comfort for sports activities in hot environment.