This study was conducted to evaluate the effect of biochar derived from greenhouse crop residues on the lettuce growth and soil chemical properties in pot experiment. The pepper stem biochar was manufactured through a controlled pyrolysis process conducted at 400°C for a duration of 2 hours. Biochar application levels were conducted with 100, 200, 400, 800, 1,000, and 2,000 kg 10a -1 and the inorganic fertilizer (N-P-K 7.0-3.0-3.6 kg 10a -1 ) were applied on the recommended fertilizer application levels. The treatments were followed: no fertilizer (NF), inorganic fertilizer (NPK), biochar treatment without inorganic fertilizer (PB), biochar treatment with inorganic fertilizer (NPK+PB). The lettuce yields in the PB treatments were no significant difference in compared to the NF treatment. The lettuce yields in the NPK+PB treatments (2,589 kg 10a -1 ) were 111% higher than in the PB treatments (1,229 kg 10a -1 ). Also, the lettuce yields in NPK+PB100, NPK+PB200, NPK+PB400, NPK+PB800, NPK+PB1000, and NPK+PB2000 treatments increased by 104, 137, 125, 141, 141, and 144% respectively, compared to the NF treatment. The nitrogen uptake of the lettuce in the NPK+PB treatments (4.5 kg 10a -1 ) was 164% higher than in the PB treatments (1.7 kg 10a -1 ). After cultivation, the soil electrical conductivity (EC) and organic matter (OM) were affected by biochar application levels. Particularly, the soil chemical properties of NPK+PB2000 (1.3 dS m -1 , 20 g kg -1 ) treatment was markedly increased compared with NF (0.7 dS m -1 , 13 g kg -1 ) treatment. These results indicated that combined application of biochar and inorganic fertilizer can improve lettuce productivity and soil chemical properties.