Effects of woody forages on biodiversity and bioactivity of aerobic culturable gut bacteria of tilapia (Oreochromis niloticus)

Wu, Feng; Chen, Biao; Liu, Sha; Xia, Xiongjian; Gao, Liuling; Zhang, Xiaoyong; Pan, Qin

doi:10.1371/journal.pone.0235560

Cited by 14 publications

(20 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, these three species recovered from tilapia intestine were reported to possess extracellular protease activity and other beneficial properties, and are being considered as probiotic candidates for Nile tilapia [37,44,45]. Although not frequently, but the other four genera identified in our analysis, Pseudomonas, Stenotrophomonas, Micrococcus and Providencia, have also been described among the intestinal bacteria of Nile tilapia [42,[46][47][48]. Whatever the source of their isolation is, species of all the seven genera were reported producing extracellular proteases [49][50][51][52][53][54][55][56].…”

Section: Discussionmentioning

confidence: 66%

“…Few of the similar phylotypes have been previously documented in the GIT of Nile tilapia. For example, species of Bacillus (B. megaterium; reclassified as Priestia megaterium), Citrobacter, and Burkholderia were commonly isolated from Nile tilapia [37,[40][41][42][43], and therefore seems to be autochthonous to this fish. Moreover, these three species recovered from tilapia intestine were reported to possess extracellular protease activity and other beneficial properties, and are being considered as probiotic candidates for Nile tilapia [37,44,45].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Hossain

Das

Ali

et al. 2021

Preprint

View full text Add to dashboard Cite

Vertebrate intestine appears an excellent source of proteolytic bacteria for industrial and probiotic use. We therefore aimed obtaining the gut-associated proteolytic species of Nile tilapia. We have isolated twenty six bacterial strains from its intestinal tract, seven of which showed exoprotease activity with the formation of clear halos on skim milk. Their depolymerization ability was further assessed on three distinct proteins including casein, gelatin and albumin. All the isolates could successfully hydrolyze the three substrates indicating relatively broad specificity of their secreted proteases. Molecular taxonomy and phylogeny of the proteolytic isolates were determined based on their 16S rRNA gene barcoding which suggested that the seven strains belong to three phyla viz. Firmicutes, Proteobacteria and Actinobacteria, distributed across the genera Priestia, Citrobacter, Pseudomonas, Stenotrophomonas, Burkholderia, Providencia and Micrococcus. The isolates were further characterized by a comprehensive study of their morphological, cultural, cellular and biochemical properties which were consistent with the phylogenetic annotations. To reveal their proteolytic capacity alongside substrate preferences, enzyme-production was determined by the diffusion assay. The Pseudomonas, Stenotrophomonas and Micrococcus isolates appeared most promising with maximum protease production on casein, gelatin and albumin media respectively. Our findings present valuable insights into the phylogenetic and biochemical properties of gut-associated proteolytic strains of Nile tilapia.

show abstract

Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Hossain

Das

Ali

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…After fed for 56 days and fasted for 24 hr (Ran et al., 2016; Wu et al., 2020), the weights of Nile tilapia fish in reference group were very close to those in four woody forage groups, and the weights of Nile tilapia fish in the four different woody forage groups were also close. Three healthy fish were randomly picked from each cage, anaesthetized with tricaine methanesulphonate (60 mg/L), killed and transported on ice to the laboratory as soon as possible (Chen et al., 2018).…”

Section: Methodsmentioning

confidence: 89%

“…For the fermented woody forages, M. oleifera (leaf) and F. mori (leaf) meals were fermented by a conventional fermentation method based on Wu et al. (2020) with modification. Briefly, the activated fungus Aspergillus sydowii was cultured on PDA (potatoes 200 g/L, glucose 20 g/L, agar 20 g/L) plates at 26°C for 6 days.…”

Section: Methodsmentioning

confidence: 99%

Effects of dietary four different woody forages on gut microbiota of Nile tilapia ( Oreochromis niloticus )

Zhang

Zeng

et al. 2020

Aquaculture Research

Self Cite

View full text Add to dashboard Cite

The present study investigated for the first time the gut bacterial responses of Nile tilapia (Oreochromis niloticus) to four different woody forages (Moringa oleifera, fermented M. oleifera, Folium mori and fermented F. mori meals) by high‐throughput Illumina sequencing. Forty‐eight bacterial phyla and a very different bacterial community consisted of Paeniclostridium, Clostridium, Mycobacterium, Synechococcus, Cetobacterium, Trachydiscus and Romboutsia were detected in this study. Interestingly, it was found that the number of bacterial operational taxonomic units (OTUs) in foreguts and hindguts of Nile tilapia in reference diet group was very close, while the number of bacterial OTUs in hindguts was much more than that in foregut of tilapia after fed with different woody forages. These results indicated that woody forages could effectively enhance the diversity and difference of gut bacterial community of tilapia. Furthermore, woody forges might influence the distribution of some unique bacterial community (Desulfovibrio, Thiobacillus, Methylobacillus, Marmoricola and Syntrophobacter) in guts of Nile tilapia, which was confirmed by comparing predicted gut bacterial functions that two pathways including membrane transport and carbon metabolism in four woody forage groups were significantly higher than those in reference diet group. This present study contributed to our understanding of the gut bacterial diversity and functions of Nile tilapia fed with different woody forages.

show abstract

“…S. warneri and S. pasteuri , for example, were reported in the intestine of Atlantic salmon in several individual research ( Bakke-McKellep et al, 2007 ; Askarian et al, 2011 ; Abid et al, 2013 ); the latter sp. was also identified in the intestine of gibel carp ( Carassius auratus ) as well as in the fresh water fish Nile tilapia ( He et al, 2011 ; Wu et al, 2020 ). Fish in those studies were, however, fed with specific diets.…”

Section: Discussionmentioning

confidence: 99%

Hydrolytic Exoenzymes Produced by Bacteria Isolated and Identified From the Gastrointestinal Tract of Bombay Duck

et al. 2020

View full text Add to dashboard Cite

Bacteria producing hydrolytic exoenzymes are of great importance considering their contribution to the host metabolism as well as for their various applications in industrial bioprocesses. In this work hydrolytic capacity of bacteria isolated from the gastrointestinal tract of Bombay duck (Harpadon nehereus) was analyzed and the enzyme-producing bacteria were genetically characterized. A total of twenty gutassociated bacteria, classified into seventeen different species, were isolated and screened for the production of protease, lipase, pectinase, cellulase and amylase enzymes. It was found that thirteen of the isolates could produce at least one of these hydrolytic enzymes among which protease was the most common enzyme detected in ten isolates; lipase in nine, pectinase in four, and cellulase and amylase in one isolate each. This enzymatic array strongly correlated to the previously reported eating behavior of Bombay duck. 16S rRNA gene sequence-based taxonomic classification of the enzyme-producing isolates revealed that the thirteen isolates were grouped into three different classes of bacteria consisting of eight different genera. Staphylococcus, representing ∼46% of the isolates, was the most dominant genus. Measurement of enzyme-production via agar diffusion technique revealed that one of the isolates which belonged to the genus Exiguobacterium, secreted the highest amount of lipolytic and pectinolytic enzymes, whereas a Staphylococcus species produced highest proteolytic activity. The Exiguobacterium sp. expressing a maximum of four hydrolases, appeared to be the most promising isolate of all.

show abstract

Effects of woody forages on biodiversity and bioactivity of aerobic culturable gut bacteria of tilapia (Oreochromis niloticus)

Cited by 14 publications

References 73 publications

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Substrate preferences, phylogenetic and biochemical properties of proteolytic bacteria present in the digestive tract of Nile tilapia

Effects of dietary four different woody forages on gut microbiota of Nile tilapia ( Oreochromis niloticus )

Hydrolytic Exoenzymes Produced by Bacteria Isolated and Identified From the Gastrointestinal Tract of Bombay Duck

Contact Info

Product

Resources

About