Effects of Wounding on Cytokinin Activity in Cucumber Cotyledons

Crane, K. E.; Ross, Cleon W.

doi:10.1104/pp.82.4.1151

Cited by 25 publications

(18 citation statements)

References 6 publications

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“…Moreover, enhanced endogenous ABA, jasmonic acid, and ethylene production were detected (León et al 2001). Wounding also enhanced the endogenous cytokinin activity (Crane and Ross 1986;Iwase et al 2011). However, the hormonal response to excision seems to be completely different than to wounding.…”

Section: Explant Excision Resulting In Altered Endogenous Hormone Conmentioning

confidence: 98%

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

Grzyb

Kalandyk

Waligórski

et al. 2017

Plant Cell Tiss Organ Cult

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of explant excision, a dramatic reduction in concentration of all phytohormones studied was observed, but hormonal balance and sugar content remained almost unchanged. During the 14-day-long culture, the ABA/CKs and ABA/ IAA ratios remained constant, whereas the greatest differences were detected for the IAA/CKs and Z-type/iPA cytokinin ratios. Excluding day 6 of culture, cytokinins were found to be the predominant phytohormones over IAA. An almost 12-fold increase in soluble sucrose concentration at day 6 of culture might be the switch to the SE expression phase. Frequent cell divisions leading to somatic embryo formation are clearly associated with increase in trans-zeatin riboside content. Keywords

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Section: Explant Excision Resulting In Altered Endogenous Hormone Conmentioning

confidence: 98%

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

Grzyb

Kalandyk

Waligórski

et al. 2017

Plant Cell Tiss Organ Cult

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“…Preliminary experiments indicated that treatment of intact seedlings with 50 #M BA was sufficient to stimulate dark growth of etiolated cucumber cotyledons as has been previously described [9,12] (Fig. 2).…”

Section: Regulation Of Phy Mrna In Response To Ba Treatmentmentioning

confidence: 99%

Down-regulation of phytochrome mRNA abundance by red light and benzyladenine in etiolated cucumber cotyledons

et al. 1990

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Northern blot analysis revealed that a single 4.2 kb phytochrome mRNA species was detectable in cotyledons excised from five-day-old etiolated cucumber seedlings. Intact etiolated five-day-old cucumber seedlings were given a red light or benzyladenine treatment, and cotyledons were harvested at various times following treatment. The abundance of phytochrome mRNA in the cotyledons was quantitated using 32P-labeled RNA probes and slot blot analysis. By 2 h after irradiation the phytochrome mRNA level was reduced to 40% of the initial abundance and reaccumulation began by 3 h after irradiation. Reaccumulation of phytochrome mRNA to the time-zero dark control level was achieved by 10 h after treatment. A decrease in phytochrome mRNA abundance was evident by 2 h after benzyladenine treatment, and a maximal reduction to 45% of the time-zero dark control was attained by 4 h after treatment. No recovery of the phytochrome mRNA level was evident by 8 h after benzyladenine treatment. The abundance of actin mRNA was unaffected by benzyladenine treatment.

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“…The levels were much lower in young leaves than in mature or senescent leaves. Cytokinins are thought to control greening [10,11,20] and aging of leaves [for review, see 12] and to mediate responses to wounding [7,14]. Thus, the expression of this gene could be closely related to the various actions of cytokinins.…”

Section: Introductionmentioning

confidence: 99%

Noncoding RNA for CR20, a cytokinin-repressed gene of cucumber

et al. 1996

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The CR20 gene was identified as a cytokinin-repressed gene in excised cotyledons of cucumber. We determined the sequences of some CR20 cDNAs with different structures and sequenced genomic clones for CR20. This gene consisted of three exons, and there were at least three types of transcript, which seemed to be generated by alternative splicing of the second intron. None of the CR20 transcripts included a long open reading frame (ORF). We isolated a cDNA of Arabidopsis thaliana with cucumber CR20 cDNA as a probe. This cDNA for a gene designated AtCR20-1 also lacked a long ORF. A region of 180 nucleotides was conserved in the CR20 RNA of cucumber and the AtCR20-1 RNA of Arabidopsis, although the homology was relatively low when the entire sequences were compared. Each conserved region consisted of seven elements, and seems to form stable secondary structure. These suggest that CR20 RNA may function as an RNA that is not translated into a protein.

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Effects of Wounding on Cytokinin Activity in Cucumber Cotyledons

Cited by 25 publications

References 6 publications

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

The content of endogenous hormones and sugars in the process of early somatic embryogenesis in the tree fern Cyathea delgadii Sternb.

Down-regulation of phytochrome mRNA abundance by red light and benzyladenine in etiolated cucumber cotyledons

Noncoding RNA for CR20, a cytokinin-repressed gene of cucumber

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