Effects of Wuji Pill with different compatibility on the activity of cytochrome P450 1A2 in rat liver microsomes <i>in vitro</i>

Weng, Xiaogang; Li, Yujie; Yang, Qing; Liang, Rixin; Wang, Yiwei; Liu, Xiaoni; Han, Xiao; Zhang, Gan‐Lin; Sui, Feng; Zhu, Xiongwei

doi:10.11569/wcjd.v18.i6.586

Cited by 5 publications

(6 citation statements)

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“…The ethanol extract of RC was prepared by the Department of Pharmacy in China-Japan Friendship Hospital and its main constituents were reported to be alkaloids. The content of berberine and palmatine in the ethanol extract of RC was 23.03% and 5.52% [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

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Pharmacokinetics of Two Alkaloids after Oral Administration of Rhizoma Coptidis Extract in Normal Rats and Irritable Bowel Syndrome Rats

Gong

Ying

Zhang

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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A comparative pharmacokinetic study of berberine and palmatine after oral administration of Rhizoma Coptidis extract (96 mg/kg, containing berberine 22 mg/kg and palmatine 5 mg/kg based on body weight) was performed in normal and postinflammation irritable bowel syndrome (PI-IBS) rats, induced by intracolonic instillation of acetic acid and restraint stress. Quantification of berberine and palmatine in rat plasma was achieved by using a sensitive and rapid UPLC-MS/MS method. Plasma samples were collected at 13 different time points and the pharmacokinetic parameters were analyzed by WinNonlin software. The significant differences in the pharmacokinetic behaviors, such as C max⁡, AUC(0–t), V d/F, and CL/F, of berberine and palmatine were found between normal and PI-IBS model rats. The results indicated that PI-IBS pathological conditions in rats could alter the pharmacokinetic behavior of drug. Preclinical pharmacokinetic studies are usually carried out on healthy animals. However, we should pay more attention to the fact that the change of pharmacokinetic behavior plays an important role on efficacy. It is essential to investigate the pharmacokinetics of the drug in disease status.

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Section: Methodsmentioning

confidence: 99%

“…Plasma concentrations of berberine and palmatine were determined by using the UPLC-MS/MS method previously developed and validated [ 20 ]. The qualitative chemical profile of these extracts was analyzed by UPLC-MS/MS as previously described [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of Two Alkaloids after Oral Administration of Rhizoma Coptidis Extract in Normal Rats and Irritable Bowel Syndrome Rats

Gong

Ying

Zhang

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Radix Astragali showed remarkable inhibitory effect on the activity of CYP3A4 in human liver microsomes and in rats (Pao et al, 2012). Only a slight inhibitory effect was observed when the rats were exposed to the extract of Radix Paeoniae Alba (Weng et al, 2010). No study has reported on the influence of Herba Ephedrae on CYP3A.…”

Section: Resultsmentioning

confidence: 99%

The significant inhibition on CYP3A caused by radix Aconiti single herb is not observed in the Wutou decoction: The necessity of combination therapy of radix Aconiti

Zhang

Chen

et al. 2015

Journal of Ethnopharmacology

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“…The Chinese Pharmacopoeia (2020 edition, Volume I) prescribes the following: Rhizoma Coptidis 300 g, Fructus Evodiae Rutaecarpae 50 g, and Radix Paeoniae Alba 300 g (6:1:6) (Chinese Pharmacopoeia Commission, 2020). Recent pharmacological studies have shown that WJW inhibits colon contraction in guinea pigs (Wang et al, 2007), inhibits CYP1A2 enzyme activity (Weng et al, 2010), significantly reduces nitric oxide content in mice infected by Helicobacter pylori (Wu et al, 2006), and has anti-colitic (Cho, et al, 1998;Küpeli,et al, 2002;Kuo,et al, 2004;Lee,et al, 2007) and antithrombotic effects (Sheu et al, 2000;Yu, et al, 2009;Jia and Hu, 2010;Yuan,et al, 2011). It is commonly used in the clinical treatment of belching, nausea, vomiting, mouth bitterness, abdominal pain, diarrhea (Chen et al, 2008a), and gastric ulcer (Chen,et al, 2008b).…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters

et al. 2022

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The Wuji pill, also called Wuji Wan (WJW), is an effective traditional medicine for the clinical treatment of irritable bowel syndrome (IBS). It is principally composed of Rhizoma Coptidis, Fructus Evodiae Rutaecarpae, and Radix Paeoniae Alba. There have been no reports on the pharmacokinetics of WJW on IBS. Because it is more meaningful to study pharmacokinetics in relation to specific pathological conditions, our study investigated the pharmacokinetic differences of five representative components (berberine, palmatine, evodiamine, rutaecarpine, and paeoniflorin) in normal rats and chronic visceral hypersensitivity IBS (CVH-IBS) model rats after single dose and multiple doses of WJW using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Transmission electron microscopy, immunohistochemistry, and immunofluorescence were used to explore mechanisms behind the pharmacokinetic differences in terms of tight junction proteins (Occludin and ZO-1), myosin light chain kinase (MLCK), and transporters including P-glycoprotein (P-gp), multidrug resistance associated protein 1 (MRP1), and multidrug resistance associated protein 2 (MRP2) in rat colons. After a single dose, for all components except rutaecarpine, significant differences were observed between normal and model groups. Compared with normal group, T1/2 and AUC0-t of berberine and palmatine in model group increased significantly (562.5 ± 237.2 vs. 1,384.9 ± 712.4 min, 733.8 ± 67.4 vs. 1,532.4 ± 612.7 min; 5,443.0 ± 1,405.8 vs. 9,930.8 ± 2,304.5 min·ng/ml, 2,365.5 ± 410.6 vs. 3,527.0 ± 717.8 min·ng/ml), while Cl/F decreased (840.7 ± 250.8 vs. 397.3 ± 142.7 L/h/kg, 427.7 ± 89.4 vs. 288.9 ± 114.4 L/h/kg). Cmax and AUC0-t of evodiamine in model group increased significantly (1.4 ± 0.6 vs. 2.4 ± 0.7 ng/ml; 573 ± 45.3 vs. 733.9 ± 160.2 min·ng/ml), while T1/2, Tmax, Cl/F, and Vd/F had no significant difference. Tmax and AUC0-t of paeoniflorin in model group increased significantly (21.0 ± 8.2 vs. 80.0 ± 45.8 min; 15,428.9 ± 5,063.6 vs. 33,140.6 ± 5,613.9 min·ng/ml), while Cl/F decreased (110.5 ± 48.1 vs. 43.3 ± 9.5 L/h/kg). However, after multiple doses, all five components showed significant differences between normal and model groups. Moreover, these differences were related to tight junction damage and the differential expression of transporters in the colon, suggesting that dose adjustment might be required during administration of WJW in the clinical treatment of IBS.

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Effects of Wuji Pill with different compatibility on the activity of cytochrome P450 1A2 in rat liver microsomes in vitro

Cited by 5 publications

References 0 publications

Pharmacokinetics of Two Alkaloids after Oral Administration of Rhizoma Coptidis Extract in Normal Rats and Irritable Bowel Syndrome Rats

Pharmacokinetics of Two Alkaloids after Oral Administration of Rhizoma Coptidis Extract in Normal Rats and Irritable Bowel Syndrome Rats

The significant inhibition on CYP3A caused by radix Aconiti single herb is not observed in the Wutou decoction: The necessity of combination therapy of radix Aconiti

Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters

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