Effects of zinc oxide nanoparticles and zinc sulfate on the testis of common carp,<i>Cyprinus carpio</i>

Seetharam, Deepa; Murugananthkumar, Raju; Gupta, Yugantak Raj; S, Manjunatha Gowda K; Senthilkumaran, B.

doi:10.1080/17435390.2018.1541259

Cited by 30 publications

(8 citation statements)

References 54 publications

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“…In our research, it was shown that 28-day gavage exposure of ZnO NPs (30 nm positively charged) at the concentrations of 100, 200, and 400 mg/kg/day caused disruption and atrophy of the seminiferous epithelium in the testis of mice. Furthermore, the sperm density in the epididymis significantly decreased in the ZnO NPs-treated groups, which was in good agreement with some previous work [22,27]. This toxic dosage range is also similar to the research of Hong et al, in which they tested the toxicity on embryo-fetal development in rats from 15 days of repeated oral doses of 20 nm negatively-charged ZnO NPs [50].…”

Section: Discussionsupporting

confidence: 91%

“…ZnO NPs have been reported to exert cytotoxic effects on mouse Leydig cells [25,26]. Similar toxic effects were revealed in the testis of six-month-old common carp Cyprinus carpio after exposure to 10, 50, and 100 μg/L ZnO NPs for 21 days [27]. Furthermore, increasing evidence suggests that the toxicity of ZnO NPs may result from ROS production [9,28,29].…”

Section: Introductionmentioning

confidence: 76%

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Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

Shen

Yang

Zhou

et al. 2019

IJMS

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Zinc oxide nanoparticles (ZnO NPs) have shown adverse health impact on the human male reproductive system, with evidence of inducing apoptosis. However, whether or not ZnO NPs could promote autophagy, and the possible role of autophagy in the progress of apoptosis, remain unclear. In the current study, in vitro and in vivo toxicological responses of ZnO NPs were explored by using a mouse model and mouse Leydig cell line. It was found that intragastrical exposure of ZnO NPs to mice for 28 days at the concentrations of 100, 200, and 400 mg/kg/day disrupted the seminiferous epithelium of the testis and decreased the sperm density in the epididymis. Furthermore, serum testosterone levels were markedly reduced. The induction of apoptosis and autophagy in the testis tissues was disclosed by up-regulating the protein levels of cleaved Caspase-8, cleaved Caspase-3, Bax, LC3-II, Atg 5, and Beclin 1, accompanied by down-regulation of Bcl 2. In vitro tests showed that ZnO NPs could induce apoptosis and autophagy with the generation of oxidative stress. Specific inhibition of autophagy pathway significantly decreased the cell viability and up-regulated the apoptosis level in mouse Leydig TM3 cells. In summary, ZnO NPs can induce apoptosis and autophagy via oxidative stress, and autophagy might play a protective role in ZnO NPs-induced apoptosis of mouse Leydig cells.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 76%

Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

Shen

Yang

Zhou

et al. 2019

IJMS

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“…Copper metal has been established to be a reproductive disruptor in zebrafish by injuring the gonads, modifying the steroid hormone concentrations, and the expressions of endocrine-related genes in the hypothalamic-pituitary-gonadal axis (Cao et al 2019). Deepa et al (2019) reported defective testicular lumen, slow progress of spermatogenesis, and increased Leydig cell degeneration in Cyprinus carpi after exposure to different forms and doses of zinc metal. Ramamoorthi et al (2008) showed that the mixture of manganese and cadmium or copper was negatively correlated with overall sperm motility, and the correlations of the combined metals were greater than that of the single metal.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Exposure to the Water of Wadi El-Rayan Lakes Induced Testicular Damage and Endocrine Disruption in Mugil cephalus

Abdel-Khalek

Al‐Quraishy

Abdel‐Gaber

2021

Bull Environ Contam Toxicol

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This study aimed to investigate the testicular function of Mugil cephalus that inhabit Wadi El-Rayan lakes. Testes of fish inhabiting the upper lake (site 2) and the lower lake (site 3) of Wadi El-Rayan showed significant decreases in gonadosomatic index, high accumulation levels of six metals, and eight organochlorine pesticide residues. Compared to reference fish, high percentages of histological alterations as testicular degeneration, germ cell reduction, testicular inflammation, vacuolization, and loss of tubular arrangement were observed in sites 2 and 3. Moreover, endocrine disruption signs were recorded based on the percentage of ovotestis appearance and the ovotestis severity index values. The maximum defective testicular antioxidant mechanisms were recorded in site 3 as indicated by sharp decreases in catalase, superoxide dismutase, glutathione reduced levels, and high thiobarbituric acid reactive substances. Finally, long-term exposure to Wadi El-Rayan water may impair the reproductive health of fish via testicular oxidative damage and endocrine disruption.

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“…In the testis of C. carpio, 100 µg L −1 ZnNPs suppressed the expression of steroidogenic enzyme genes (20β-hsd, cyp19a1) and transcription factors (dmrt1, activin β, dax1, foxt2, ad4bp, wnt5) [64].…”

Section: Effects Of Other Nanoparticulate Metals On Gene Expressionmentioning

confidence: 98%

Changes of Gene Expression Patterns from Aquatic Organisms Exposed to Metal Nanoparticles

Kulasza

Skuza

2021

IJERPH

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Metal nanoparticles are used in various branches of industry due to their physicochemical properties. However, with intensive use, most of the waste and by-products from industries and household items, and from weathering of products containing nanoparticles, end up in the waters. These pollutants pose a risk to aquatic organisms, one of which is a change in the expression of various genes. Most of the data that focus on metal nanoparticles and their effects on aquatic organisms are about copper and silver nanoparticles, which is due to their popularity in general industry, but information about other nanoparticulate metals can also be found. This review aims to evaluate gene expression patterns in aquatic organisms by metal nanoparticles, specifying details about the transcription changes of singular genes and, if possible, comparing the changes in the expression of the same genes in different organisms. To achieve this goal, available publications tackling this problem are studied and summarized. Nanometals were found to have a modulatory effect on gene expression in different aquatic organisms. Data show both up-regulation and down-regulation of genes. Nano silver, nano copper, and nano zinc show a regulatory effect on genes involved in inflammation and apoptosis, cell cycle regulation and ROS defense as well as in general stress response and have a negative effect on the expression of genes involved in development. Nano gold, nano titanium, nano zinc, and nano iron tend to elevate the transcripts of genes involved in response to ROS, but also pro-apoptotic genes and down-regulate DNA repair-involved genes and anti-apoptotic-involved genes. Nano selenium showed a rare effect that is protective against harmful effects of other nanoparticles, but also induced up-regulation of stress response genes. This review focuses only on the effects of metal nanoparticles on the expression of various genes of aquatic organisms from different taxonomic groups.

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Effects of zinc oxide nanoparticles and zinc sulfate on the testis of common carp,Cyprinus carpio

Cited by 30 publications

References 54 publications

Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

Long-Term Exposure to the Water of Wadi El-Rayan Lakes Induced Testicular Damage and Endocrine Disruption in Mugil cephalus

Changes of Gene Expression Patterns from Aquatic Organisms Exposed to Metal Nanoparticles

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