Effects of α-aminooxyacetic acid on the level of polyamines, anthocyanins and photosynthetic pigments in seedlings of common buckwheat (Fagopyrum esculentum Moench)

Abstract

The present paper discusses the effects of α-aminooxyacetic acid (AOA) on contents of polyamines, anthocyanins, photosynthetic pigments and phenylalanine ammonia-lyase activity in seedlings of common buckwheat (Fagopyrum esculentum Moench). AOA clearly decreased light-induced formation of anthocyanins and inhibited PAL activity in buckwheat hypocotyls, although a slight stimulatory effect on anthocyanins content in buckwheat cotyledons was observed. AOA declined the contents of chlorophylls a and b, and total carotenoids in buckwheat cotyledons. The results show that AOA inhibits phenylpropanoids biosynthesis in buckwheat hypocotyls, and suppress photosynthesis in cotyledons. Moreover, the experiments show that AOA enhances the level of free putrescine in hypocotyls and the level of spermidine in buckwheat cotyledons. AOA also diminished the content of putrescine in cotyledons, but did not affect its level in buckwheat hypocotyls. AOA also substantially declined the level of cadaverine in buckwheat cotyledons, and did not affect its content in hypocotyls. Differences in effect of AOA on anthocyanins and polyamines accumulation indicate various physiological roles of the compounds in buckwheat hypocotyls and cotyledons.

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

“…This was probably due to other activities of ethylene inhibitors in plant metabolism. AOA has also been found to be an inhibitor of phenylalanine ammmonia lyase (PAL), the first enzyme in the phenylpropanoid pathway (Horbowicz et al, 2011). The study on Arabidopsis indicated that AVG had a negative effect on ACC synthase activity, and also reduced the endogenous IAA content (Soeno et al, 2010).…”