Effects of α-Galactosidase Supplementation on Performance and Energy Metabolism for Broilers Fed Corn-non-dehulled Soybean Meal Diets

Abstract

To study the effects of α-galactosidase (α-Gal) supplementation on performance and energy metabolism, 216 Arbor Acres male broilers were placed in 36 cages of 6 birds each and allotted to 4 diets for 42 d, with 0-21 d as starter period and 22-42 d as grower period. The 4 diets were based on corn non-dehulled soybean meal in a 2×2 factorial arrangement, with 2 levels of α-Gal (0 vs. 60 U/kg feed) and 2 levels of ME (normal metabolizable energy (NME) and low metabolizable energy (LME)). Bird performance was obtained at 21 and 42 d of age with samples of feces collected for nutrient digestibility from 19-21 d and 40-42 d. At 21 and 42 d, 1 bird from 6 cages of each treatment was killed to determine liver weight, intestinal pH and chyme viscosity. With the addition of α-Gal the 42 d body weight (BW) and 0-42 d average daily gain (ADG) were significantly improved (p<0.05). Average daily feed intake (ADFI) of birds fed the LME diet was significantly increased compared to those fed the NME diet during starter (p<0.01) and grower (p<0.05) periods and overall (p<0.01). There was an interaction of α-Gal×ME on 0-21 d ADFI (p<0.01). Supplementation of α-Gal significantly improved (p<0.01) feed efficiency during the grower period and overall. Feed efficiency of birds fed the LME diet was significantly decreased (p<0.05) compared to those fed the NME diet during the starter period and overall. With the addition of α-Gal apparent metabolizable energy (AME) was improved (p<0.01) by 2.1% and 1.8% during starter and grower periods, respectively. There was a main effect (p<0.05) of α-Gal on the digestion of neutral detergent fiber (NDF) during the starter period and crude protein (CP), NDF and acid detergent fiber (ADF) during the grower period. With the addition of α-Gal, the relative weight of liver was reduced (p<0.01) during the two phases. The duodenal and jejunal pH were significantly decreased (p<0.01) with the supplementation of α-Gal at the two phases. α-Gal addition reduced (p<0.01) chyme viscosity of the ileum during the starter and grower periods. Overall, α-Gal showed a major effect on nutrient efficiency, improved ADG and feed efficiency, whereas LME decreased feed efficiency. The incorporation of α-Gal into a LME diet could at least partially offset ME deficiency of non-dehulled soybean meal.

Effect of β-Mannanase and α-Galactosidase Supplementation to Soybean Meal Based Diets on Growth, Feed Efficiency and Nutrient Digestibility of Rainbow Trout, &lt;i&gt;Oncorhynchus mykiss&lt;/i&gt; (Walbaum)

“…However, SBM is low in some amino acids and contains antinutritional factors ( Tacon 1995 ; El-Sayed, 1999 ; Krogdahl et al, 2010 ; Hagely et al, 2013 ). The SBM contains α-galactosides that cannot be digested in the intestine of monogastrics including fish, due to the absence of α - galactosidase ( Refstie et al, 1998 ; Waldroup et al, 2006 ; Zhang et al, 2010 ; Sørensen et al, 2011 ; Hagely et al, 2013 ). In addition, SBM contains approximately 13 to 20 g/kg β - mannans ( Dierick 1989 ; Mehri et al, 2010 ; Mok et al, 2013 ).…”

A Mini Review of the Effects of NSP and Exogenous Enzymes in Broiler Diets on Digestibility and Some Intestinal Functions

“…The Van Soest procedure (Van Soest et al, 1991) obtains three fiber fractions, NDF (neutral detegent fiber), ADF (acid detergent fiber) and hemicelluloses (NDF-ADF), while only NDF corresponds to dietary fiber. Certain authors investigating the effects of fiber and the effects of exogenous enzymes acting on NSP on the digestion of nutrients estimated digestibility of fiber fraction through digestibility of NDF, ADF and hemicellulose (Vukić Vranješ et al, 1994;Jamroz et al, 2002;Ao et al, 2009;Zhang et al, 2010;Nian et al, 2011). However, some parts of soluble NSP are lost through dissolution during the aqueous treatment.…”

Effect of Domestic Processing and Crude Extract of α-Galactosidase on Oligosaccharide Content of Red Gram (Cajanus cajan L.) Seeds

“…30 Zhang W et al, 31 have reported the complete hydrolysis of raffinose and stachyose to galactose within 6 hours time period at 50 0 C by α-Galactosidase enzyme from Termitomyces eurrhizus. Zhang B et al, 32 have reported the effect of α-Galactosidase treatment on energy metabolism for broilers fed on corn-soybean meal diet.…”