Effects of α, β momorcharin extract of momordica charantia in intracellular free calcium on cancer cell lines.

Abstract

The Momordica charantia L., (family: Cucurbitaceae) is a scientific name of the plant and its fruit. It is also known by other names, for instance in the USA it is known as Bitter gourd or balsam pear while its referred to as the African cucumber in many African countries. This study was specifically designed to investigate the cellular mechanisms whereby alpha, beta momorcharin an extract of M. charantiacan induce cell death measuring the elevation in intracellular free calcium concentrations in three different cancer cell lines 1321N1, Gos-3 and U-87. The results show that incubation of the three cancer cell lines 1321N1, Gos-3 and U-87 with α, β momorcharin can result in significant (p < 0.05) time-dependent increases in [Ca2+]i in all three cancer cell lines compared to control (untreated) cells. Maximal increases in [Ca2+]i was attained after 420 min of incubation.In control (untreated cell lines), [Ca2+]i remained more or less stable in both cell lines after 420 min. The results also show that the increase in [Ca2+]i in Gos-3 cell line was much more pronounced following incubation with α, β momorcharin compared to 1321N1 and U-87 cell line. The results show that incubation of the three cancer cell lines with momorcharin can result in significant (p < 0.05) time-dependent increases in [Ca2+]i in all three cancer cell lines compared to control (untreated) cells. Maximal increases in [Ca2+]i was attained after 420 min of incubation. In control (untreated cell lines), [Ca2+]i remained more or less stable in all three cell lines after 420 min. These results clearly show that α, β momorcharin extract of M. charantia is exerting its anti- cancer effect via an insult to the mitochondria resulting in apoptosis, calcium overloading and subsequently, cell death.

The treatment of cancer by influenced of alpha and beta momorcharin

“…Paclitaxel is a home grown item with antitumor distraction. Paclitaxel is a diterpene alkaloid gotten from the dried bark of Paci ic yew tree Taxus brevifolia [6][7][8]. The synthetic name for paclitaxel is 5β, 20-Epoxy-l, 2α, 4, 7β, 10β,13α-hexahydroxytax -l-en-nine-one four,10-diacetate 2-benzoate thirteen-ester with (2R,3S)-Nbenzoyl-three-phenylisoserine.…”

Effects of alpha, beta momorcharin fruit extract with the combination of paclitaxel in the treatment of glioma cancer in-vivo.

“…Paclitaxel is a natural product with antitumor activity. Paclitaxel is a diterpene alkaloid derived from the dried bark of Pacific yew tree Taxus brevifolia [7][8][9]. The chemical name for paclitaxel is 5β, 20-Epoxy-l, 2α, 4, 7β, 10β,13αhexahydroxytax-l l-en-9-one 4,10-diacetate 2-benzoate 13-ester with (2R,3S)-N-benzoyl-3-phenylisoserine.…”

“…The chemical name for paclitaxel is 5β, 20-Epoxy-l, 2α, 4, 7β, 10β,13αhexahydroxytax-l l-en-9-one 4,10-diacetate 2-benzoate 13-ester with (2R,3S)-N-benzoyl-3-phenylisoserine. Paclitaxel is a white to off-white crystalline powder with the empirical formula C 47 H 51 NO 14 and a molecular weight of 853.9 g/mol [9,10]. Paclitaxel is highly lipophilic, and melts at around 216-217°C and is used in treatment of various advanced carcinomas such as ovarian cancer, lung cancer, breast cancer, acute leukemias, head and neck cancers and Kaposi's sarcoma [11][12][13].…”