Effects of β-1,3/1,6 glucan dietary supplements on some immunological and hematological health markersin Siberian sturgeon ( Acipenser baerii) infected with Aeromonas hydrophila

Abstract

This experimental study aimed to investigate some hematological and immunological changes as a result of Aeromanas hydrophila infection in Siberian sturgeon (Acipenser baerii Brandt, 1869). Their feeds were supplemented with β-1,3/1,6 glucan at different ratios, 250 mg/kg (βG 250 ); 500 mg/kg (βG 500 ) and 750 mg/kg (βG 750 ). To create an experimental infection, 4×10 6 cfu/ml Aeromonas hydrophila inoculum was intraperitoneally injected to fish. 0.1 ml intraperitoneal bacteria injection was given to the fish in 12 of 15 tanks, each consisting of 10 fish (the fish in the control group were not given bacteria). Considering the βG 500 and βG 750 group fish as positive (C+) and negative control (C−) groups in terms of hematological parameters, it was found that RBC (erythrocyte) and Hb (hemoglobin) values, as well as RBC indices (MCV, MCHC, MCH) significantly increased. The immunological parameters, including WBC (leukocyte), leukocyte cell percentages (lymphocyte, monocyte, neutrophil, eosinophil), as well as cytokines, including IL-1β, IL-6, IL-8, and TNF-α values showed similar increases in the βG 500 and βG 750 groups. It was found that the addition of 500 and 750 mg/kg doses of β-1,3/1,6 glucan to the feed stimulated non-specific immunity of fish against bacterial agents and/or septicemic diseases and beta glucan at this dosage range was determined to be ideal for fish health and that it may be a herbal immunostimulant that can be an alternative to many medicaments.

High Starch Induces Hematological Variations, Metabolic Changes, Oxidative Stress, Inflammatory Responses, and Histopathological Lesions in Largemouth Bass (Micropterus salmoides)

“…Hematological parameters have been important physiological assays for analyzing pathological variations in human and fish species [31,46,47]. Our results showed that high starch increased RBC counts and HGB levels, especially in the D1, D3 and D5 groups, which was in line with former results in Siberian sturgeon (Acipenser baerii) [48]. It is well-known that RBC and HGB undertake key roles in increasing oxygen-carrying, metabolic capacity and redox regulation in humans and animals [49][50][51].…”

Hematological and Hematopoietic Analysis in Fish Toxicology—A Review

“…Toxic agents, e.g., metal ions, pesticides, or other anthropogenic aquatic pollutants, as well as pharmaceuticals such as immunomodulators, antimicrobial and antiparasitic therapeutics, or anesthetics, were proven to cause hematological changes in fish [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. To observe toxicity-induced alterations, it is necessary to provide reference values.…”