Collagen, a widely used biomaterial, is susceptible to
denaturation
during production from native tissues, posing serious challenges for
its applications in tissue engineering. Accurate quantification of
denatured collagen (DC) is essential for evaluating the quality of
collagen-based biomaterials, yet quantitative methods for assessing
collagen denaturation are lacking. Here, we for the first time present
a highly specific biochip for sensitive quantification of denatured
collagen levels (L
dc), addressing this
critical need in collagen quality analysis. The denatured collagen-specific
chip (DCSC) features an intrinsically nontrimerizing peptide probe,
F-GOP-14, targeting denatured collagen and a fully denatured collagen-coated
capture surface. The DCSC demonstrates exceptional sensitivity and
accuracy in quantifying DC concentration (C
dc) and total collagen concentration (C
tc), enabling precise calculation of L
dc. Importantly, DCSC is versatile, detecting L
dc across various denaturing scenarios, including UV radiation,
thermal environments, and decellularization. This denatured collagen-specific
biochip offers a robust method for accurately analyzing L
dc, with significant potential for enhancing collagen
quality assessment in biomaterial development and production.