Effects on growth performance, faecal microflora and plasma cholesterol after supplementation of spray-dried metabolite to postweaning rats

Abstract

ABSTRACT:A study was conducted to study the effects of feeding a spray-dried metabolite (SDM) produced by Lb. plantarum I-UL4 in the diets of postweaning rats on growth performance, faecal pH, Enterobacteriaceae bacterial and lactic acid bacteria (LAB) counts in the faeces and on plasma cholesterol concentrations. A total of 15 female 4-weeks-old Sprague dawley rats were randomly assigned to 3 groups of diets: basal diet (control), 0.25% SDM and 0.5% SDM diets. Daily feed intake, daily growth rate, blood plasma for total cholesterol concentration and faecal Enterobacteriaceae and LAB were measured. The growth rate, total feed intake, feed conversion ratio, and pH were not significantly different (P > 0.05) among all the treatment groups. The faecal Enterobacteriaceae counts in the 0.25% and 0.5% groups were significantly (P < 0.05) lower than in the control group. However, there was no significant difference (P > 0.05) in the LAB counts among all the treatment groups. The plasma cholesterol concentration was significantly reduced (P < 0.05) following the feeding of the metabolite. The control had the highest concentration of cholesterol. However, the 0.5% SDM group had the lowest plasma cholesterol concentration, followed by the 0.25% SDM group.

Effects of Metabolite Combinations Produced by <i>Lactobacillus plantarum</i> on Plasma Cholesterol and Fatty Acids in Piglets

“…This is complement with Loh et al (2009) when they reported that feeding 0.25% spray-dried metabolite was able to reduce the plasma cholesterol concentration in postweaning rats. Fermented milk containing L. acidophilus L1 had effects in reducing 2.4-3.2% of serum cholesterol concentration in human (Lee et al, 2009).…”

“…The cholesterol concentration was measured by enzymatic method using the cholesterol diagnostic kit (Randox Laboratories Ltd, UK). This procedure was based on the method as described by Loh et al (2009). The determination method of fatty acids was modified from Oliveira et al (2009).…”

“…Therefore, reduction of risk factors from diet which associated with cardiovascular disease has much attention in human as well as in animals. It has been reported that LAB able to reduce plasma cholesterol when used as an additive in animal feeding (Sindhu and Khetarpaul, 2003;Loh et al, 2009). Lee et al (2009) showed Lactobacillus plantarum could reduce the plasma cholesterol.…”

“…Recently, metabolites of LAB containing lactic, acetic, propionic, butyric acids, ethanol and bacteriocins were able to increase faecal LAB, volatile fatty acids and decrease faecal pH, plasma cholesterol in rats and chickens (Foo et al, 2005;Thanh et al, 2009). Moreover, Loh et al (2009) reported that feeding of spray-dried metabolites from L. plantarum reduced plasma cholesterol and increased essential fatty acids in rats. However, the effects of feeding metabolites on plasma cholesterol and fatty acid concentrations in piglets are still unknown yet.…”

Fatty Acid Profile, Cholesterol and Oxidative Status in Broiler Chicken Breast Muscle Fed Different Dietary Oil Sources and Calcium Levels

“…The total fatty acids were extracted from breast muscles based on the method Folch et al (1957), described by Loh et al (2009), and modified by Ebrahimi et al (2014), using chloroform : methanol (2 : 1, v/v) containing butylated hydrotoluene to prevent oxidation during sample preparation. The extracted fatty acids were transmethylated to their fatty acid methyl esters (FAME) using 0.66 N KOH in methanol and 14% methanolic boron trifluoride (BF 3 ) (Sigma Chemical Co., St. Louis, Mo, USA) according to the methods by AOAC (1990).…”