Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders

Santiani, Alexei; Huanca, W.; Sapana, R.; Huanca, Teodosio; Sepúlveda, Néstor; Sánchez, Raúl

doi:10.1111/j.1745-7262.2005.00021.x

Cited by 62 publications

(74 citation statements)

References 14 publications

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“…La criopreservación de semen de alpaca ha tenido un progreso pobre y desalentador comparado con otras especies domesticas (Vaughan et al, 2003;Santiani et al, 2005). El uso reciente de espermatozoides del epidídimo para desarrollar técnicas de congelamiento de semen en alpacas han cambiado la percepción sobre el espermatozoide de alpaca y brinda luces en la fisiología de esta célula.…”

Section: Discussionunclassified

“…Además, el semen es generalmente pobre en calidad y altamente viscoso, haciendo difícil su manejo, dilución y evaluación. Trabajos sobre criopreservación de semen de alpaca reportan entre 6 y 20% de motilidad posdescongelamiento (Vaughan et al, 2003;Santiani et al, 2005) y raramente superan el 40%, aunque Bravo et al (1996) reportaron 45%, pero sin dar detalles que permitan la réplica de su metodología.…”

Section: Introductionunclassified

“…La mayoría de trabajos sobre criopreservación de espermatozoides de alpaca ha empleado glicerol (6-7%) como agente crioprotector, donde los resultados demuestran una recuperación pobre tras la descongelación, acompañada con la pérdi-da parcial de la motilidad inicial (Vaughan et al, 2003;Santiani et al, 2005;Valdivia et al, 2005). Recientemente, una metodología eficiente de criopreservación de espermatozoides epididimarios de alpaca ha sido desarrollada utilizando glicerol a una concentración de 3% (Morton et al, 2007 …”

Section: Introductionunclassified

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Agentes Crioprotectores Alternativos para el Congelamiento Lento de Espermatozoides Epididimarios de Alpaca (Vicugna pacos)

Pariona

Arnedo

Valdivia

2015

Rev. investig. vet. Perú

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RESUMENEl objetivo del estudio fue utilizar agentes crioprotectores alternativos para el congelamiento lento de espermatozoides epididimarios de alpaca (Vicugna pacos). Epidídimos obtenidos en el camal de la ciudad de Huancavelica fueron transportados a 4 °C en solución fisiológica al laboratorio en Lima, Perú. Los espermatozoides fueron extraídos de la cola del epidídimo e incubados en medio HAMF10 a 37 °C. Se usó el medio Tes-Tris-Yolk como medio de criopreservación y suplementado con los agentes crioprotectores dimetilsulfóxido (Me 2 SO 4 ) en concentraciones de 0.5, 0.25 y 0.125M y dimetilacetamida (DMA) en concentraciones de 0.75, 0.385 y 0.18M, y comparados con el grupo control criopreservado con glicerol 0.6M. Se evaluó la motilidad, viabilidad e integridad de membrana antes y después del congelamiento. Se obtuvo una mejor viabilidad e integridad de membrana posdescongelamiento con DMA 0.375M (p<0.05). La motilidad fue mejor en los grupos de Me 2 SO 4 0.25 y 05M y de DMA 0.375 y 0.75M (p<0.05).Palabras clave: alpaca, espermatozoide, criopreservación, dimetilacetamida, dimetilsulfóxido ABSTRACTThe objective of this study was to use alternative cryoprotectans suitable for freezing epididymal spermatozoa of alpaca (Vicugna pacos) in a slow freezing method. Epididymides obtained in the slaughterhouse of Huancavelica city were transported at

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Section: Discussionunclassified

Section: Introductionunclassified

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Agentes Crioprotectores Alternativos para el Congelamiento Lento de Espermatozoides Epididimarios de Alpaca (Vicugna pacos)

Pariona

Arnedo

Valdivia

2015

Rev. investig. vet. Perú

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“…Sperm motility was estimated at 80% before freezing, 60% after final dilution and cooling (before freezing), and 30-40% after thawing. Most recently [37], ethylene glycol was used as a cryoprotectant for alpaca semen collected by AV, liquefied by mechanical action, and diluted with skim milk and fructose. Post-thaw motility was 30% and highly correlated with estimates of live sperm and the acrosome reaction.…”

Section: Semen Extender Cooling and Freezingmentioning

confidence: 99%

Artificial insemination in South American camelids and wild equids

et al. 2009

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An overview of the present status of the use of artificial insemination (AI) in South American camelids and wild equids is offered. Technical aspects of semen collection, dilution and cryopreservation have limited the development and use of AI in camelid and equid species. To-date, efficiency is low but progress has been made and viable offspring have been produced through the use of AI in domestic South American camelids using both fresh and frozen semen. The origin, composition, and function of the viscous component of camelid seminal plasma remain a mystery and an obvious area for future research. A better understanding of the normal constituents of seminal plasma will enable the rational design of semen extenders suitable for camelids. Post-thaw sperm viability is very low, and studies are needed to address questions of optimal freezing and thawing procedures as well as the insemination dose. The basis for differences in reported pregnancy rates with sexed and frozen semen in domestic equids, and the ultimate success of AI in wild equids will require continued research into the ''stallion effect'', extenders and cryoprotectants, optimal volume and number of spermatozoa, temperatures during handling, processing an transport, and insemination techniques. In both camelids and equids, research on domestic species under controlled conditions provides and excellent opportunity to develop effective semen handling techniques for application in wild and endangered species of the respective families. #

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“…The successful recovery of motile sperm from frozen samples varies widely [3][4][5]. Factors influencing sperm recovery include the method of freezing and thawing as well as the choice and concentration of cryoprotectants [1,3,5,6]. Glycerol in combination with eggyolk is by far the most widely used and successful cryoprotectant for human sperm [2,5].…”

Section: Introductionmentioning

confidence: 99%

Sole Use of Sucrose in Human Sperm Cryopreservation

Hossain

Osuamkpe

2007

Archives of Andrology

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Effects on the quality of frozen-thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders

Abstract: The skim milk- and egg yolk-based extenders containing ethylene glycol or glycerol to freeze alpaca semen seems to promote the survival of more sperm cells with intact acrosomes than the other extenders.

Cited by 62 publications

References 14 publications

Agentes Crioprotectores Alternativos para el Congelamiento Lento de Espermatozoides Epididimarios de Alpaca (Vicugna pacos)

Agentes Crioprotectores Alternativos para el Congelamiento Lento de Espermatozoides Epididimarios de Alpaca (Vicugna pacos)

Artificial insemination in South American camelids and wild equids

Sole Use of Sucrose in Human Sperm Cryopreservation

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