Effects on UDP glucuronosyl transferase, glutathione transferase, DT-diaphorase and glutathione reductase activities in rainbow trout liver after long-term exposure to PCB

Förlin, Lars; Blom, Sonja; Celander, Malin; Sturve, Joachim

doi:10.1016/0141-1136(95)00061-5

Cited by 22 publications

(6 citation statements)

References 11 publications

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“…Increasing activity of CAT was demonstrated in response to exposure to organochlorines and PCBs in Perna viridis and Ruditapes philippinarum (De Luca-Abbott et al 2005). Among different invertebrates exposed to PCBs and PAHs, induction of GST and GPx activities were observed (Förlin et al 1996;Machala et al 1998;Martin-Diaz et al 2008;Martín-Díaz et al 2007). MT were used as biomarkers of heavy metals exposure on several organisms including vertebrates and invertebrates: mice Mus spretus (Marques et al 2008), fish Scatophagus argus (Nikpour et al 2008), crab Carcinus maenas (Pedersen et al 1997), mussel Mytilus edulis (Geffard et al 2005), and clam Ruditapes decussates (Hamza-Chaffai et al 1999).…”

Section: Introductionmentioning

confidence: 87%

SOD and CAT cDNA cloning, and expression pattern of detoxification genes in the freshwater bivalve Unio tumidus transplanted into the Moselle river

2009

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The cDNA sequences encoding manganese superoxide dismutase (Mn-SOD) and catalase (CAT) were isolated in the freshwater bivalve Unio tumidus by reverse-transcription polymerase chain reaction (RT-PCR) using degenerate primers. Quantitative real-time PCR approach was used to evaluate the mRNA expression patterns of SOD, CAT, selenium-dependent glutathione peroxidase (Se-GPx), pi class glutathione S-transferase (pi-GST) and metallothionein (MT), in the digestive gland of Unio tumidus transplanted from a control site to four stations in the Moselle River (M1-M4), for periods of 8 and 21 days. These sites were chosen upstream and downstream of populated areas. Chemical analysis performed on sediments from the Moselle river sites did not show high levels of pollutants. Decrease of SOD, CAT, Se-GPx and MT mRNA levels were observed at M3 site after a 21-day exposure compared to control site. These results suggest inefficiency of antioxidant systems affected by cytotoxic mechanisms and confirm an environmental perturbation. Organisms transplanted at M4 site showed a strong increase of biomarkers transcription levels after 21 days of exposure. These inductions could correspond to an adaptive response to an altered environment. Our results showed that biological approaches using multibiomarkers appear as essential tools complementary to measurement of contaminants, to detect environmental degradations.

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Section: Introductionmentioning

confidence: 87%

SOD and CAT cDNA cloning, and expression pattern of detoxification genes in the freshwater bivalve Unio tumidus transplanted into the Moselle river

2009

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“…However, few changes in gsr were statistically significant. One study on rainbow trout found that exposure to PCBs caused an increase in phase II enzyme and GSR activity (Förlin et al 1996). Increased enzyme activity of GSR can occur during oxidative stress in littoral fish exposed to a number of environmental contaminants, such PCBs, metals, and PAHs; it was suggested that antioxidant activities acted as a protection mechanism during chronic oxidative stress (Rodriguez-Ariza et al 1993).…”

Section: Discussionmentioning

confidence: 99%

15. The Comparative Toxicity of two Canadian Diluted Bitumens to Developing Yellow Perch (Perca flavescens)

McDonnell¹

2018

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Increasing demand for diluted bitumen (dilbit) has led to the development of the Alberta oil sands industry and the expansion of current and future transcontinental pipelines. However, the growth of oil transportation has led to public concern about the effects of potential dilbit spills to aquatic ecosystems. Although the toxic effects of crude oils through exposure to polycyclic aromatic hydrocarbons (PAH) are well characterized, little is known about the toxic effects of dilbit because of the variable proportions of diluent added to bitumen. Here we assessed the toxicity of the two most transported dilbits in Canada, Access Western Blend (AWB) and Cold Lake Blend (CLB) to developing yellow perch (Perca flavescens), a species distributed throughout North America. Embryos were exposed to dilbit until hatch, or up to 16 days, using a static daily renewal treatment regime of water accommodated fractions (WAF) and chemically-enhanced water accommodated fractions (CEWAF) of dilbit at total PAH (TPAH) concentration ranges of 0.02 to 10.7 μg/L and 0.21 to 20.4 μg/L TPAH, respectively. Results show that with increased TPAH concentration, the frequency of hatched embryos with developmental malformations increased proportionally. Expression of genes associated with phase I and II detoxification, cellular stress, and xenobiotic metabolism were altered in higher TPAH concentrations. This is the first study assessing the toxicity of both AWB and CLB dilbits on wild-sourced fish. With recent approvals of pipelines in North America, these biomarkers will assist risk assessments and monitoring of Canadian ecosystems should a pipeline spill occur.

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“…Pretti et al [40] reported low DTD activities in gilthead seabream ( Sparus auratus ) (0.7 nmol min -1 mg -1 in control fish) and observed no elevation in DTD activity after exposing the fish to a high dose (50 mg Kg -1 ) of β-NF. Previous studies in rainbow trout exposed to the AHR agonist 3-MC and β-NF showed increased hepatic DTD activities [26,27]. …”

Section: Discussionmentioning

confidence: 99%

“…Some AHR agonists can also induce DTD activity in rainbow trout. Förlin et al [26] observed in a long term study of rainbow trout that polychlorinated biphenyls (PCB) and 3-methylcholanthrene (3-MC) caused an increase in DTD activity. It has previously been demonstrated that also β-NF induces DTD activity in rainbow trout [27].…”

Section: Discussionmentioning

confidence: 99%

Effects of redox cycling compounds on DT diaphorase activity in the liver of rainbow trout (Oncorhynchus mykiss)

2005

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Background: DT diaphorase (DTD; NAD(P)H:quinone oxidoreductase; EC 1.6.99.2) catalyses the two electron reduction of quinones, thus preventing redox cycling and consequently quinone dependent production of reactive oxygen species. In rat and mouse, a wide range of chemicals including polyaromatic hydrocarbons, azo dyes and quinones induces DTD. Bifunctional compounds, such as β-naphthoflavone (β-NF) and benzo(a)pyrene (B(a)P), induce DTD together with CYP1A and phase II enzymes by a mechanism involving the aryl hydrocarbon receptor (AHR). Monofunctional induction of DTD is mediated through the antioxidant response element and does not lead to the induction of AHR dependent enzymes, such as CYP1A. The aim of this study was to investigate the effects of prooxidants (both bifunctional and monofunctional) on the activity of hepatic DTD in rainbow trout (Oncorhynchus mykiss) in order to evaluate DTD suitability as a biomarker. We also investigated the effect of β-NF on hepatic DTD activity in perch (Perca fluviatilis), shorthorn sculpin (Myoxocephalus scorpius), eelpout (Zoarces viviparus), brown trout (Salmo trutta) and carp (Cyprinus carpio). In addition, the effect of short term exposure to prooxidants on catalase activity was investigated.

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Effects on UDP glucuronosyl transferase, glutathione transferase, DT-diaphorase and glutathione reductase activities in rainbow trout liver after long-term exposure to PCB

Cited by 22 publications

References 11 publications

SOD and CAT cDNA cloning, and expression pattern of detoxification genes in the freshwater bivalve Unio tumidus transplanted into the Moselle river

SOD and CAT cDNA cloning, and expression pattern of detoxification genes in the freshwater bivalve Unio tumidus transplanted into the Moselle river

15. The Comparative Toxicity of two Canadian Diluted Bitumens to Developing Yellow Perch (Perca flavescens)

Effects of redox cycling compounds on DT diaphorase activity in the liver of rainbow trout (Oncorhynchus mykiss)

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