Effects of dietary fat on dairy cows are reviewed. Dietary fat did not affect gain in BW or body condition score after peak lactation but tended to increase BW loss during early lactation and body fat deposition in growing cattle. Dietary fat decreased de novo fatty acid synthesis in adipose tissue. Basal FFA release from adipose tissue in vitro and beta-adrenergic lipolytic responses were increased by protected polyunsaturated fatty acids. Dietary fat increased body fat in growing pigs and decreased BW loss in lactating sows. Dietary fat decreased de novo fatty acid synthesis and basal glycerol release in adipose tissue and tended to increase simultaneously beta-adrenergic lipolytic responses to increased membrane fluidity. Dietary fat increased body fat in rats. Polyunsaturated fatty acids were sometimes less efficient than saturated ones in increasing body fat. Lipoprotein lipase activity in adipose tissue generally decreased. Hepatic fatty acid synthesis was decreased sharply by polyunsaturated fatty acids, and adipose tissue response was less important. beta-Adrenergic-stimulated lipolysis decreased, and fatty acid esterification increased, particularly from saturated fatty acids. A trend toward insulin resistance, which was more marked with saturated fatty acids, occurred in adipose tissue.