Effets de la réduction artificielle du blastocèle avant vitrification en système fermé : étude contrôlée randomisée

Gala, Anna; Ferrières, A.; Assou, Saïd; Monforte, Mauro; Bringer-Deutsch, S.; Vintejoux, Emmanuelle; Vincens, C.; Brunet, C.; Hamamah, S.

doi:10.1016/j.gyobfe.2014.09.006

Cited by 8 publications

(5 citation statements)

References 43 publications

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“…Our observation of warmed blastocysts started with assessment of the proportion of intact cells in blastocysts. Blastocyst survival rate after warming in this study can be compared only with that from studies where the same vitrification and warming kits and protocols were used (Gala et al, 2014;Van Landuyt et al, 2015). In the study by Van Landuyt et al (2015) the blastocysts with more than 50% intact cells immediately after warming were judged as suitable for transfer.…”

Section: Discussionmentioning

confidence: 99%

“…In the study by Van Landuyt et al (2015) the blastocysts with more than 50% intact cells immediately after warming were judged as suitable for transfer. In the study by Gala et al (2014), only completely lysed blastocysts were assessed as non-vital. Despite the different criteria used, both groups reported significantly higher survival rates in artificially collapsed blastocysts.…”

Section: Discussionmentioning

confidence: 99%

“…Today, there is considered to be a lack of evidence as to whether or not blastocysts benefit from artificial shrinkage before vitrification. A trend towards better clinical results has been observed in two prospective randomized clinical trials (Gala et al, 2014;Van Landuyt et al, 2015). These studies did not show a significant effect on implantation and pregnancy, but did demonstrate improved survival after artificial blastocoel collapse.…”

Section: Introductionmentioning

confidence: 89%

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Artificial blastocoel collapse of human blastocysts before vitrification and its effect on re-expansion after warming – a prospective observational study using time-lapse microscopy

Kovačič

Taborin

Vlaisavljević³

2018

Reproductive BioMedicine Online

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Vitrified human blastocysts show varied re-expansion capacity after warming. This prospective observational study compared behaviour of artificially collapsed blastocysts (study group patients, n = 69) to that of blastocysts that were vitrified without artificial collapse (control group patients, n = 72). Warmed blastocysts were monitored by time-lapse microscopy and blastocoel re-expansion speed and growth patterns compared between study and control groups. These parameters were also retrospectively compared between blastocysts that resulted in live birth and those that failed. Artificially collapsed blastocysts re-expanded on average 15.01 µm/min faster than control blastocysts (P = 0.0013). Warmed blastocysts expressed four different patterns of blastocoel growth. The pattern showing contractions at the end of culture was observed to have a lower prevalence in control blastocysts, which coincided with the lower incidence of hatching in this group. Re-expansion speed and prevalence of growth patterns were comparable between blastocysts that did and did not result in a live birth. This was seen in the study and control groups. Despite faster re-expansion and different growth patterns of artificially collapsed blastocysts, live birth rate did not differ between groups. However, this result should be interpreted with caution due to the small sample size and high risk of bias.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

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Artificial blastocoel collapse of human blastocysts before vitrification and its effect on re-expansion after warming – a prospective observational study using time-lapse microscopy

Kovačič

Taborin

Vlaisavljević³

2018

Reproductive BioMedicine Online

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“…The embryo transfer strategy used in this study has been previously described [9]. It takes into account the maternal age, her antecedents, the number of previous attempts and the score of the obtained embryos.…”

Section: Methodsmentioning

confidence: 99%

Sperm quality and paternal age: effect on blastocyst formation and pregnancy rates

Chapuis¹,

Gala

Ferrières-Hoa

et al. 2017

Basic Clin. Androl.

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BackgroundSeveral studies suggest a decrease in sperm quality in men in the last decades. Therefore, the aim of this work was to assess the influence of male factors (sperm quality and paternal age) on the outcomes of conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).MethodsThis retrospective study included all couples who underwent IVF or ICSI at Montpellier University Hospital, France, between 1 January 2010 and 31 December 2015. Exclusion criteria were cycles using surgically retrieved sperm or frozen sperm, with pre-implantation genetic diagnosis or using frozen oocytes. The primary outcomes were the blastulation rate (number of blastocysts obtained at day 5 or day 6/number of embryos in prolonged culture at day 3) and the clinical pregnancy rate. The secondary outcomes were the fertilization and early miscarriage rates.ResultsIn total, 859 IVF and 1632 ICSI cycles were included in this study. The fertilization rate after ICSI was affected by oligospermia. Moreover, in ICSI, severe oligospermia (lower than 0.2 million/ml) led to a reduction of the blastulation rate. Reduced rapid progressive motility affected particularly IVF, with a decrease of the fertilization rate and number of embryos at day 2 when progressive motility was lower than 32%.Paternal age also had a negative effect. Although it was difficult to eliminate the bias linked to the woman’s age, pregnancy rate was reduced in IVF and ICSI when the father was older than 51 and the mother older than 37 years.ConclusionsThese results allow adjusting our strategies of fertilization technique and embryo transfer. In the case of severe oligospermia, transfer should be carried out at the cleaved embryo stage (day 2–3) due to the very low blastulation rate. When the man is older than 51 years, couples should be aware of the reduced success rate, especially if the woman is older than 37 years. Finally, promising research avenues should be explored, such as the quantification of free sperm DNA, to optimize the selection of male gametes.

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“…Artificial shrinkage (AS) of the blastocoel by different methods-laser pulse, microneedle, micropipetting, and 29-gauge needle was developed with the idea of overcoming this obstacle. A study by Gala et al in 2014 encompassing 185 warming cycles reported a higher survival rate after AS (99.0%) compared to 91.8% survival rate in the control group without AS [46]. Darwish and Magdi in 2016 assessed clinical pregnancy rates, implantation rates, and blastocyst survival rates in more than 400 patients, divided into two groups-untreated expanded blastocysts and blastocysts undergone AS by laser pulse [47].…”

Section: Artificial Shrinkagementioning

confidence: 99%

The Present and Future of Embryo Cryopreservation

Vladimirov¹,

Tacheva²,

Dobrinov³

2019

Embryology - Theory and Practice

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Embryo freezing technologies have widely been used in human IVF practice and in animal industry. In this chapter, we will review the development of embryo freezing technology and the application of the method, which will concentrate on discussion of the arguments in favor of and against freezing, as well as the latest results of success rates, comparing them with the other basic assisted reproductive technologies methods. Then, we will present our viewpoint for the future application of embryo freezing methods and their place in reproductive medicine. The analysis of facts and suggestions should enable researchers to rethink the position of cryobiology in reproductive medicine. It should be considered that the method of cryopreservation is not only a technology for storing embryos but also a method of embryo treatment that can potentially improve the success rates in infertile couples. There is also a theory that describes the "treatment" effect of freezing an embryo, which may explain the higher success rates of frozen embryo transfer (FET) compared to fresh embryo transfer (ET). The authors of the "Theory about the Embryo-Cryo treatment" believe that freezing and thawing could activate endogenous survival and repair mechanisms in preimplantation embryos.

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Effets de la réduction artificielle du blastocèle avant vitrification en système fermé : étude contrôlée randomisée

Cited by 8 publications

References 43 publications

Artificial blastocoel collapse of human blastocysts before vitrification and its effect on re-expansion after warming – a prospective observational study using time-lapse microscopy

Artificial blastocoel collapse of human blastocysts before vitrification and its effect on re-expansion after warming – a prospective observational study using time-lapse microscopy

Sperm quality and paternal age: effect on blastocyst formation and pregnancy rates

The Present and Future of Embryo Cryopreservation

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