Efficacy and mechanism of the antinociceptive effects of cannabidiol on acute orofacial nociception induced by Complete Freund’s Adjuvant in male Mus musculus mice

Wanasuntronwong, Aree; Kaewsrisung, Supassanan; Rotpenpian, Nattapon; Arayapisit, Tawepong; Pavasant, Prasit; Supronsinchai, Weera

doi:10.1016/j.archoralbio.2022.105570

Cited by 4 publications

(5 citation statements)

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“…The novel object recognition (NOR) task was employed to evaluate object recognition memory which relies on mice’s natural tendency to explore novel stimuli ( Philpott et al, 2017 ; Wanasuntronwong et al, 2022 ). The task was conducted within a hexagonal arena constructed of lightly textured high-density white polyethylene (50 cm wide, 23 cm tall, courtesy of G.F. League Co., Inc., Greenville, SC).…”

Section: Methodsmentioning

confidence: 99%

“…The task was conducted within a hexagonal arena constructed of lightly textured high-density white polyethylene (50 cm wide, 23 cm tall, courtesy of G.F. League Co., Inc., Greenville, SC). The task encompassed three phases: habituation, training, and testing, as previously detailed ( Wanasuntronwong et al, 2022 ). Briefly, mice were individually habituated to the testing apparatus for 5 min and returned to their home cage.…”

Section: Methodsmentioning

confidence: 99%

“…Total exploration time (s) of both objects (familiar and novel) was calculated to determine object exploration time. A discrimination index (DI) was calculated according to the formula: DI = [t(novel) – t(familiar)]/[t(novel) + t(familiar)] for the testing phase ( Jesus et al, 2019 ; Wanasuntronwong et al, 2022 ). A discrimination index of 0 indicates no preference, 1 indicates a complete preference for the novel object, and − 1 indicates a complete preference for the familiar object.…”

Section: Methodsmentioning

confidence: 99%

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Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice

Yadav-Samudrala,

Gorman,

Barmada

et al. 2024

Front. Neurosci.

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BackgroundSome evidence suggests that cannabidiol (CBD) has potential to help alleviate HIV symptoms due to its antioxidant and anti-inflammatory properties. Here we examined acute CBD effects on various behaviors and the endocannabinoid system in HIV Tat transgenic mice.MethodsTat transgenic mice (female/male) were injected with CBD (3, 10, 30 mg/kg) and assessed for antinociception, activity, coordination, anxiety-like behavior, and recognition memory. Brains were taken to quantify endocannabinoids, cannabinoid receptors, and cannabinoid catabolic enzymes. Additionally, CBD and metabolite 7-hydroxy-CBD were quantified in the plasma and cortex.ResultsTat decreased supraspinal-related nociception and locomotion. CBD and sex had little to no effects on any of the behavioral measures. For the endocannabinoid system male sex was associated with elevated concentration of the proinflammatory metabolite arachidonic acid in various CNS regions, including the cerebellum that also showed higher FAAH expression levels for Tat(+) males. GPR55 expression levels in the striatum and cerebellum were higher for females compared to males. CBD metabolism was altered by sex and Tat expression.ConclusionFindings indicate that acute CBD effects are not altered by HIV Tat, and acute CBD has no to minimal effects on behavior and the endocannabinoid system.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice

Yadav-Samudrala,

Gorman,

Barmada

et al. 2024

Front. Neurosci.

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“…The finding of reduced pain sensitivity for the supraspinal hot plate test but not the spinal-related tail flick test may be explained by a previous study that found higher Tat mRNA expression in the striatum compared to the spinal cord [105]. For acute CBD exposure, no effects on pain sensitivity in Tat transgenic mice were found at any dose given (3, 10, 30 mg/kg), even though other studies demonstrated attenuation of pain sensitivity by acute CBD treatment of similar doses in various other preclinical rodent models [107][108][109]. To our knowledge, cannabidiol's effects on pain have not been studied in PLWH and/or neuroHIV mouse models, including the Tat transgenic mouse model.…”

Section: Discussionmentioning

confidence: 79%

Effects of Acute Cannabidiol on Behavior and the Endocannabinoid System in HIV-1 Tat Transgenic Female and Male Mice

Yadav-Samudrala,

Gorman,

Barmada

et al. 2023

Preprint

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(1) Background: Some evidence suggests that cannabidiol (CBD) has potential to help alleviate HIV symptoms due to its antioxidant and anti-inflammatory properties. Here we examined acute CBD effects on various behaviors and the endocannabinoid system in HIV Tat transgenic mice. (2) Methods: Tat transgenic mice (female/male) were injected with CBD (3,10,30 mg/kg) and assessed for antinociception, activity, coordination, anxiety, and recognition memory. Brains were taken to quantify endocannabinoids and related lipids. Additionally, CBD and metabolite 7-hydroxy-CBD were quantified in the plasma and cortex. (3) Results: Tat decreased striatal-related nociception and locomotion, with some sex-dependent effects on coordination. CBD had no effect on nociception and coordination, but increased locomotor activity. For anxiety, differential CBD effects were noted for sex, with decreasing anxiety-like behavior in males only. In the striatum and spinal cord, male sex was associated with lower 2-arachidonoylglycerol and with elevated concentrations of its proinflammatory metabolite arachidonic acid. CBD metabolism was altered by sex and Tat. (4) Conclusion: Findings indicate that acute CBD effects are not altered by HIV Tat, and CBD has minimal effects on behavior and the endocannabinoid system. Interestingly, sex-dependent alterations were noted for endocannabinoids and related lipids, which may be of relevance in view of potential CBD-based treatment options for people living with HIV.

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“…Limited work has also been described investigating effects of phytocannabinoid treatment in rodent models of orofacial pain. Wanasuntronwong et al [5] investigated the efficacy and mechanisms of the non-CB 1 /CB 2 acting phytocannabinoid cannabidiol (CBD) on orofacial nociception induced by complete Freund's adjuvant (CFA) injected into the masseter muscle in mice. They found that CFA-induced spontaneous pain-like behaviors were inhibited by the administration of CBD and that this effect was mediated in part by activity at the vanilloid receptor 1 (TRPV1).…”

Section: Introductionmentioning

confidence: 99%

Non-Psychoactive Cannabinoid Modulation of Nociception and Inflammation Associated with a Rat Model of Pulpitis

Laks,

Li,

Ward

2023

Biomolecules

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Despite advancements in dental pain management, one of the most common reasons for emergency dental care is orofacial pain. Our study aimed to determine the effects of non-psychoactive Cannabis constituents in the treatment of dental pain and related inflammation. We tested the therapeutic potential of two non-psychoactive Cannabis constituents, cannabidiol (CBD) and β-caryophyllene (β-CP), in a rodent model of orofacial pain associated with pulp exposure. Sham or left mandibular molar pulp exposures were performed on Sprague Dawley rats treated with either vehicle, the phytocannabinoid CBD (5 mg/kg i.p.) or the sesquiterpene β-CP (30 mg/kg i.p.) administered 1 h pre-exposure and on days 1, 3, 7, and 10 post-exposure. Orofacial mechanical allodynia was evaluated at baseline and post-pulp exposure. Trigeminal ganglia were harvested for histological evaluation at day 15. Pulp exposure was associated with significant orofacial sensitivity and neuroinflammation in the ipsilateral orofacial region and trigeminal ganglion. β-CP but not CBD produced a significant reduction in orofacial sensitivity. β-CP also significantly reduced the expression of the inflammatory markers AIF and CCL2, while CBD only decreased AIF expression. These data represent the first preclinical evidence that non-psychoactive cannabinoid-based pharmacotherapy may provide a therapeutic benefit for the treatment of orofacial pain associated with pulp exposure.

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Efficacy and mechanism of the antinociceptive effects of cannabidiol on acute orofacial nociception induced by Complete Freund’s Adjuvant in male Mus musculus mice

Cited by 4 publications

References 46 publications

Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice

Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice

Effects of Acute Cannabidiol on Behavior and the Endocannabinoid System in HIV-1 Tat Transgenic Female and Male Mice

Non-Psychoactive Cannabinoid Modulation of Nociception and Inflammation Associated with a Rat Model of Pulpitis

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