Efficacy and Safety of Biodegradable Microparticles in the Regeneration of Injured Rabbit Corpus Cavernosum: Primary Report

Kim, Young-Joon; Lee, Sung Joo; Yoon, Hana; Chung, Wo Sik; Kim, Jeoung Yong; Shin, Mi Jung; Kang, Won Hwa; Kim, Gun Poong

doi:10.5534/kja.2011.29.3.223

Cited by 1 publication

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“…Compared to ADSC, the preparation of SVF is even simpler, with single protease digestion yielding 2–6 × 10 6 SVF cells (CSVF) per 1 mL of fat with ≥90% cell viability. Many studies have shown that ADSC and CSVF can promote tissue repairs, such as skeletal tissue [ 28 , 29 , 30 , 31 ], muscle tissue [ 32 , 33 , 34 ], nerve damage [ 35 , 36 , 37 , 38 ], and cardiovascular injury [ 39 , 40 , 41 ]. Meanwhile, the tissue source of ADSC and CSVF is widely available, and a large amount of adipose tissue can be obtained for ADSC and CSVF extraction during liposuction, manual liposuction, etc., while the invasive procedure in obtaining bone marrow MSC and muscle-derived stem cells have limited their application in clinic.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Mesenchymal Stem Cells, Adipose Tissue Derived Stem Cells, and Cellular Stromal Vascular Fraction on the Repair of Acute Anal Sphincter Injury in Rats

Chen

et al. 2022

Bioengineering

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Background: Anal sphincter incontinence (ASI) can cause a serious decline in the quality of life and can cause a socioeconomic burden. Studies have shown that bone marrow mesenchymal stem cells (MSC) have significant therapeutic effects on ASI, but the cost and risk of MSC harvest limit their further application. In contrast, adipose tissue derived stem cells (ADSC) and cellular stromal vascular fraction (CSVF) as stem cell sources have multipotency and the advantage of easy harvest. Objective: Here we aim to investigate the effects of ADSC and CSVF on treating ASI and compare them to that of bone marrow MSC. Methods: Bone marrow MSC, ADSC, and CSVF were obtained and labeled with green fluorescent protein (GFP), and CSVF was labeled with DIL. Sprague Dawley (SD) rats were divided into 5 groups. Four groups were injected with 0.2 mL phosphate buffer saline (PBS), 1 × 107/0.2 mL of MSC, ADSC, or CSVF, respectively, after model establishment. The control group received no treatment. The repair was assessed by anal functional tests and immunostaining on day 5 and day 10 after injection. Results: MSC, ADSC, and CSVF significantly promoted tissue repair and the recovery of muscle contraction and electromyographic activity in ASI. The generation of myosatellite cells by injected MSC, ADSC, and CSVF was found in the wounded area. On day 5, CSVF showed highest therapeutic effect, while on day 10, MSC and ADSC showed higher therapeutic effects than CSVF. When comparing the effects of MSC and ADSC, ADSC was slightly better than MSC in the indexes of anal pressure, etc. Conclusion: ADSC and CVSF are alternative stem cell sources for ASI repair.

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Section: Introductionmentioning

confidence: 99%

The Effect of Mesenchymal Stem Cells, Adipose Tissue Derived Stem Cells, and Cellular Stromal Vascular Fraction on the Repair of Acute Anal Sphincter Injury in Rats

Chen

et al. 2022

Bioengineering

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Efficacy and Safety of Biodegradable Microparticles in the Regeneration of Injured Rabbit Corpus Cavernosum: Primary Report

Abstract: = Abstract =Purpose: This study analyzed the effectiveness of poly (lactic-co-glycolic acid) (PLGA) as a tissue recovery agent and determines the in vivo safety and efficacy of microparticle-based PLGA.

Cited by 1 publication

References 18 publications

The Effect of Mesenchymal Stem Cells, Adipose Tissue Derived Stem Cells, and Cellular Stromal Vascular Fraction on the Repair of Acute Anal Sphincter Injury in Rats

The Effect of Mesenchymal Stem Cells, Adipose Tissue Derived Stem Cells, and Cellular Stromal Vascular Fraction on the Repair of Acute Anal Sphincter Injury in Rats

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