Efficacy and Safety of Cream Containing Climbazole/Piroctone Olamine for Facial Seborrheic Dermatitis: A Single-Center, Open-Label Split-Face Clinical Study

Youn, Hae Jeong; Kim, S.; Park, Minji; Jung, Won Hee; Lee, Yang Won; Choe, Yong Beom; Ahn, Kyu Joong

doi:10.5021/ad.2016.28.6.733

Cited by 17 publications

(16 citation statements)

References 24 publications

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“…Notably, our findings might prompt a shift towards a more targeted therapeutic approach in case of Malassezia ‐related skin diseases based on the understanding of biofilm structure and organisation, which might be more complex than previously reported for other species . Although SkinEthic ™ RHE small contains different epidermal classes of lipids comprising ceramides, M furfur and M sympodialis biofilm highly increased in amount whenever lipids were added to the yeast suspension, which supports that clinical approaches to reduce seborrhoea might affect the development of biofilm.…”

Section: Discussionsupporting

confidence: 69%

Malassezia colonisation on a reconstructed human epidermis: Imaging studies

Pedrosa

Lisboa

Branco

et al. 2019

Mycoses

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Summary Background Biofilm formation represents a major microbial virulence attribute especially at epithelial surfaces such as the skin. Malassezia biofilm formation at the skin surface has not yet been addressed. Objective The present study aimed to evaluate Malassezia colonisation pattern on a reconstructed human epidermis (RhE) by imaging techniques. Methods Malassezia clinical isolates were previously isolated from volunteers with pityriasis versicolor and seborrhoeic dermatitis. Yeast of two strains of M furfur and M sympodialis were inoculated onto the SkinEthic™ RHE. The tissues were processed for light microscopy, wide‐field fluorescence microscopy and scanning electron microscopy. Results Colonisation of the RhE surface with aggregates of Malassezia yeast entrapped in a multilayer sheet with variable amount of extracellular matrix was unveiled by imaging techniques following 24, 48, 72 and 96 hours of incubation. Whenever yeast were suspended in RPMI medium supplemented with lipids, the biofilm substantially increased with a dense extracellular matrix in which the yeast cells were embedded. Slight differences were found in the biofilm architectural structure between the two tested species with an apparently higher entrapment and viscosity in M furfur biofilm. Conclusion Skin isolates of M furfur and M sympodialis were capable of forming biofilm in vitro at the epidermal surface simulating in vivo conditions. Following 24 hours of incubation, without added lipids, rudimental matrix was barely visible, conversely to the reported at plastic surfaces. The amount of biofilm apparently increased progressively from 48 to 96 hours. A structural heterogeneity of biofilm between species was found.

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Section: Discussionsupporting

confidence: 69%

Malassezia colonisation on a reconstructed human epidermis: Imaging studies

Pedrosa

Lisboa

Branco

et al. 2019

Mycoses

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“…Moreover, the tested product contains low concentrations of piroctone olamine and climbazole, two antifungal compounds used for their preservative function only. Youn et al’s study shows that the above antifungal agents are effective to inhibit the growth of several Malassezia species including M. restricta and M. globose 30. Despite the fungal component of the skin microbiota was not investigated in this study, it cannot be excluded that the beneficial properties of piroctone olamine and climbazole may contribute to alleviating the SD symptomatology.…”

Section: Discussionmentioning

confidence: 80%

<p>Effects of a cream containing 5% hyaluronic acid mixed with a bacterial-wall-derived glycoprotein, glycyrretinic acid, piroctone olamine and climbazole on signs, symptoms and skin bacterial microbiota in subjects with seborrheic dermatitis of the face</p>

Puviani¹,

Campione²,

Offidani³

et al. 2019

CCID

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Objective: A new cream formulation containing hyaluronic acid 5%, complexed with a mix of a bacterial-wall-derived glycoprotein and peptide glycan complex (EDS), has been recently developed. We evaluated in a prospective, assessor-blinded, 6-week study the efficacy and tolerability of EDS in the treatment of facial seborrheic dermatitis (SD) and the effects on skin microbiota. Subjects and methods: Seventy-five subjects (mean age 46; 60 men) with moderate-severe SD of the face were enrolled. EDS cream was applied twice daily. The primary outcome was the evolution of the Investigator Global Assessment (IGA) score, evaluating erythema, scale/flaking, grade of seborrhea and itch. Superficial skin bacterial microbiome at baseline and after treatment was assessed, using the 16S rRNA gene methodology, in affected and non-affected face areas. Local tolerability was evaluated checking self-reported side effects at each visit. Results: Baseline IGA scores (mean±SD) was 10±3. The use of EDS reduced IGA score significantly by 70% at week 3 and by 88% at week 6. An increase in the abundance of Cutibacterium acnes genera associated with a significant drop of Staphylococcus genera presence was detected in affected areas. The ratio of relative abundance of genera Cutibacterium /Staphylococcus increased significantly after treatment in affected areas. The product was very well tolerated. Conclusion: Treatment with EDS applied twice daily for 6 consecutive weeks was associated with a reduction of the signs and symptoms of SD. Furthermore, after EDS cream treatment, a reequilibrating effect on facial skin microbiota was observed. The product was very well tolerated.

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“…Notably, our findings might prompt a shift towards a more targeted therapeutic approach in case of Malassezia-related skin diseases based on the understanding of biofilm structure and organization, which might be more complex than previously reported for other non-lipid dependent species. [9] Although SkinEthic TM RHE small contains different epidermal classes of lipids comprising ceramides, [20] M. furfur and M. sympodialis biofilm highly increased in amount whenever lipids were added to the yeast suspension, which supports that clinical approaches to reduce seborrhea [28] might affect the development of biofilm.…”

Section: Discussionmentioning

confidence: 88%

Malassezia interaction with a reconstructed human epidermis: imaging studies

Pedrosa

Lisboa

Branco

et al. 2019

Preprint

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BackgroundBiofilm formation represents a major microbial virulence attribute especially at epithelial surfaces such as the skin. Malassezia biofilm formation at the skin surface has not yet been addressed.ObjectiveThe present study aimed to evaluate Malassezia interaction with a reconstructed human epidermis (RhE) model.MethodsMalassezia clinical isolates were previously isolated from volunteers with pityriasis versicolor and seborrheic dermatitis. Yeasts of two strains of M. furfur and M. sympodialis were inoculated onto the SkinEthic™ RHE. The tissues were processed for light microscopy, wide-field fluorescence microscopy and scanning-electron microscopy.ResultsColonization of the RhE surface with aggregates of Malassezia yeasts entrapped in a multilayer sheet with variable amount of extracellular matrix was unveiled by imaging techniques following 24, 48, 72 and 96 hours of incubation. Whenever yeasts were suspended in RPMI medium supplemented with lipids, the biofilm substantially increased with a dense extracellular matrix in which the yeast cells were embedded (not seen in control samples). Slight differences were found in the biofilm architectural structure between the two tested species.ConclusionSkin isolates of M. furfur and M. sympodialis were capable of forming biofilm in vitro at the epidermal surface simulating in vivo conditions. Following 24 hours of incubation, without added lipids, rudimental matrix was barely visible, conversely to the reported at plastic surfaces. The amount of biofilm apparently increased progressively from 48 to 96 hours. A structural heterogeneity of biofilm between species was found with higher entrapment by a denser and more gelatinous extracellular matrix in M. furfur biofilm.

show abstract

Efficacy and Safety of Cream Containing Climbazole/Piroctone Olamine for Facial Seborrheic Dermatitis: A Single-Center, Open-Label Split-Face Clinical Study

Cited by 17 publications

References 24 publications

Malassezia colonisation on a reconstructed human epidermis: Imaging studies

Malassezia colonisation on a reconstructed human epidermis: Imaging studies

<p>Effects of a cream containing 5% hyaluronic acid mixed with a bacterial-wall-derived glycoprotein, glycyrretinic acid, piroctone olamine and climbazole on signs, symptoms and skin bacterial microbiota in subjects with seborrheic dermatitis of the face</p>

Malassezia interaction with a reconstructed human epidermis: imaging studies

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