Efficacy and safety of P11-4 for the treatment of periodontal defects in dogs

Abstract

Objectives This study’s aim was to investigate the safety and performance of a self-assembling peptide matrix (SAPM) P11-4 for the treatment of periodontal disease in a controlled pre-clinical study. Materials and methods Acute buccal bony dehiscence defects (LxW: 5 × 3 mm) were surgically created on the distal root of four teeth on one mandible side of 7 beagle dogs followed by another identical surgery 8 weeks later on the contralateral side. SAPM P11-4 (with and without root conditioning with 24% EDTA (T1, T2)), Emdogain® (C) and a sham intervention (S) were randomly applied on the four defects at each time point. Four weeks after the second surgery and treatment, the animals were sacrificed, the mandibles measured by micro-computed tomography (µ-CT) and sections of the tissue were stained and evaluated histologically. Results Clinically and histologically, no safety concerns or pathological issues due to the treatments were observed in any of the study groups at any time point. All groups showed overall similar results after 4 and 12 weeks of healing regarding new cementum, functionality of newly formed periodontal ligament and recovery of height and volume of the new alveolar bone and mineral density. Conclusion A controlled clinical study in humans should be performed in a next step as no adverse effects or safety issues, which might affect clinical usage of the product, were observed. Clinical relevance The synthetic SAPM P11-4 may offer an alternative to the animal-derived product Emdogain® in the future.

Therapeutic and Metagenomic Potential of the Biomolecular Therapies against Periodontitis and the Oral Microbiome: Current Evidence and Future Perspectives

“…Two systematic reviews that investigated enamel matrix derivatives in periodontal therapy concluded that enamel matrix could both significantly improve clinical attachment levels and result in reduced pocket probing depth compared to open flap debridement alone [120,121]. The EMD appears to positively influence PDL cells, cementoblasts, and osteoblasts while inhibiting epithelial cells-a characteristic favorable for the re-establishment of the periodontal tissues [122]. Another review on this topic, which only analyzed studies conducted on human samples, suggests that, although several studies have been conducted to evaluate the efficacy of the EMD in terms of periodontal wound healing and complications, further investigation is needed [123].…”

Evaluation of Feline Permanent Canine Tooth Mineral Density Using Micro-Computed Tomography

“…In Veterinary Medicine, to our knowledge, there are no studies on MD of dental tissues in canine and feline species. However, a study was recently published that integrated the measurement of MD of the alveolar bone in dogs using mCT [ 11 ]. The determination of feline teeth MD using the proposed technique could be helpful in the characterization of dental pathology in cats, such as developmental abnormalities such as enamel hypoplasia or acquired ones such as odontoclastic tooth resorption, or neoplastic lesions with dental involvement.…”