Composite materials based on polypropylene modified with silver nanoparticles (PP/AgNPs) were manufactured using injection molding and extrusion. Two different matrices were used to prepare the samples consisting of 0.5 and 1.0 wt. % of silver nanoparticles, respectively. The aim of this study was to assess whether silver nanoparticles (AgNPs) could influence the stability of a polymer matrix during the 24-month period of the in vitro testing. The results indicated that composites with silver nanoparticles displayed the significantly higher Young modulus and tensile strength after the first and second year of investigation. Moreover, the incorporation of nanoparticles into the matrix slightly increased the roughness and contact angle values and the parameters remained stable after the in vitro incubation. The two-year immersion of materials in the deionized water proved that the microstructure of composites did not change. The DSC analysis revealed that the material incubation resulted in a slight reduction in the melting temperature and degree of crystallinity of PP. The addition of nanoparticles to polymer matrices led to the increase in content of β crystals in the crystalline phase of PP, which was revealed in the long-term in vitro tests. The XRD measurement also showed the heightened surface crystallinity. The conducted studies have proved that all composites are stable over a period of 24 months. Such behavior suggests that the tested materials can be used as biomaterials.