Efficacy Assessment of Autodissemination Using Pyriproxyfen-Treated Ovitraps in the Reduction of Dengue Incidence in Parañaque City, Philippines: A Spatial Analysis

Abstract

Dengue is one of the most important vector-borne diseases worldwide and is a significant public health problem in the tropics. Mosquito control continues to be the primary approach to reducing the disease burden and spread of dengue virus (DENV). Aside from the traditional larviciding and adulticiding interventions, autodissemination using pyriproxyfen-treated (AD-PPF) ovitraps is one of the promising methods to complement existing vector control strategies. Our paper assessed the efficacy of AD-PPF in reducing DENV infections in two barangays in Parañaque City. Using saliva samples from the participants from both the control and intervention sites, we collected the seroprevalence data for three months in each of the two years. Spatial analysis was conducted to determine hotspot areas and identify DENV infection distributions across the trial periods. The results showed that the intervention site was identified as having a clustering of DENV infections in Month 0 of Year 1 and shifted to a random dispersion of dengue cases at the end of Month 3 in Year 2. The disappearance of the clustering of the intervention site translates to a decrease in the cases of DENV infection relative to the control site. Furthermore, we also identified that DENV transmission occurred at a small-scale level that did not go beyond 86 m. In conclusion, AD-PPF is suggested to be an effective strategy and may be used as an additional vector control approach, albeit based on this short-term implementation.

The impact of non-lethal doses of pyriproxyfen on male and female Aedes albopictus reproductive fitness

“…Autodissemination strategies have been suggested as a potential novel method for the control of Ae. albopictus ( 3 , 5 – 13 ). Two approaches have been successful in reducing natural populations of Aedes spp.…”

“…The first is autodissemination augmented by males (ADAM), which utilizes laboratory- or factory-reared male mosquitoes that are treated with an insect growth regulator (IGR) and released into the natural environment, where they can directly deliver an IGR to cryptic larval habitats or indirectly by copulating with naturally occurring females ( 14 , 15 ). The second is autodissemination stations, which rely on attracting females to a station wherein they are then exposed to non-lethal doses of an IGR and leave the station to disseminate the IGR to cryptic larval habitats ( 5 , 7 , 10 , 11 , 13 , 16 – 18 ).…”