Efficacy of a DNA vaccine delivered in attenuated Salmonella typhimurium against Eimeria tenella infection in chickens

Du, Aifang; Wang, Suhua

doi:10.1016/j.ijpara.2005.03.005

Cited by 64 publications

(32 citation statements)

References 37 publications

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“…Several genes coding microneme proteins of E. tenella have been identified and cloned (Ryan et al 2000). The microneme proteins from E.tenella, Etmic2 and Etmic4 have shown protective effect (Du and Wang 2005) and in ovo vaccination with Etmic2 gene reportedly stimulated intestinal protective immunity against E. tenella and E. acervulina . Administration of short oligodeoxy nucleotide containing unmethylated CpG motifs (CpG ODNs), which are known to enhance both innate and adaptative immune responses (Krieg 1995), along with the MIC2 antigen in ovo has shown promising results .…”

Section: Recombinant Vaccinementioning

confidence: 99%

Control of poultry coccidiosis: changing trends

Tewari

Maharana

2011

J Parasit Dis

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Coccidiosis is the most important protozoan disease affecting the poultry industry worldwide. Control of poultry coccidiosis is presently based on managerial skills and the use of prophylactic coccidiostatic drugs. With the emergence of drug resistant Eimeria strains, emphasis has been laid on development and use of safer vaccines; some of them have been commercialized successfully. The present review deals with the various factors responsible for the development of clinical coccidiosis in poultry as well as an overview of the currently available inducers and boosters of immunity against coccidiosis. There are three groups of vaccines currently available against coccidiosis which can be distinguished on the basis of characteristics of the Eimeria species included in the respective products, viz. vaccines based on live virulent strains, vaccines based on live attenuated strains, and vaccines based on live strains that are relatively tolerant to the ionophore compounds. The latter vaccine combines the early chemotherapeutic effect of ionophores with the late prophylactic effect of vaccination. Although in the near future more varieties of oocyst based live vaccines are expected, identification of selective coccidian-specific immunoprotective molecules is likely to get more attention to facilitate the sustainable control of poultry coccidiosis.

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Section: Recombinant Vaccinementioning

confidence: 99%

Control of poultry coccidiosis: changing trends

Tewari

Maharana

2011

J Parasit Dis

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“…In recent years, several studies have demonstrated the feasibility of using attenuated Gram-positive and Gram-negative intracellular bacteria as live vectors for the oral delivery of recombinant vaccine antigens [20], [21]. Several Salmonella enterica Typhimurium strains submitted to attenuation procedures lost their pathogenicity but remained invasive and are used as live vectors for delivery of foreign antigens.…”

Section: Introductionmentioning

confidence: 99%

Vaccination of Mice with Salmonella Expressing VapA: Mucosal and Systemic Th1 Responses Provide Protection against Rhodococcus equi Infection

et al. 2010

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Conventional vaccines to prevent the pneumonia caused by Rhodococcus equi have not been successful. We have recently demonstrated that immunization with Salmonella enterica Typhimurium expressing the VapA antigen protects mice against R. equi infection. We now report that oral vaccination of mice with this recombinant strain results in high and persistent fecal levels of antigen-specific IgA, and specific proliferation of the spleen cells of immunized mice in response to the in vitro stimulation with R. equi antigen. After in vitro stimulation, spleen cells of immunized mice produce high levels of Th1 cytokines and show a prominent mRNA expression of the Th1 transcription factor T-bet, in detriment of the Th2 transcription factor GATA-3. Following R. equi challenge, a high H2O2, NO, IL-12, and IFN-γ content is detected in the organs of immunized mice. On the other hand, TNF-α and IL-4 levels are markedly lower in the organs of vaccinated mice, compared with the non-vaccinated ones. The IL-10 content and the mRNA transcription level of TGF-β are also higher in the organs of immunized mice. A greater incidence of CD4+ and CD8+ T cells and B lymphocytes is verified in vaccinated mice. However, there is no difference between vaccinated and non-vaccinated mice in terms of the frequency of CD4+CD25+Foxp3+ T cells. Finally, we show that the vaccination confers a long-term protection against R. equi infection. Altogether, these data indicate that the oral vaccination of mice with S. enterica Typhimurium expressing VapA induces specific and long-lasting humoral and cellular responses against the pathogen, which are appropriately regulated and allow tissue integrity after challenge.

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“…There are some data in the literature to suggest that complete immunity to Eimeria infections requires prior exposure to viable oocysts with sporozites capable of invading epithelial cells and carrying out some intracellular metabolism (Jenkins, Seferian, Augustine, & Danforth, 1993) and possibly schizony (Rose & Hesketh, 1976). However, potential vaccines based on Eimeria antigens have been demonstrated to provide partial protection to infection (Crane et al, 1991;Du & Wang, 2005). A vaccine derived from the whole oocyst and lacking pathogenicity upon primary infection without fecal shedding would be desirable.…”

Section: Discussionmentioning

confidence: 99%