Efficacy of a new attenuated duck parvovirosis vaccine in Muscovy ducks

Maurin-Bernaud, L.; Goutebroze, Sylvain; Merdy, Olivier; Chanay, A.; Cozette, V.; Gros, F-X. Le

doi:10.1136/vr.102584

Cited by 10 publications

(3 citation statements)

References 21 publications

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“…Moreover, vaccination against MDPV should be applied if large production facilities are to be established. Vaccines that are based on inactivated or attenuated GPV or MDPV strains have been applied in Europe [37][38][39]. Until a local vaccine can be developed, these vaccines should be imported into Indonesia from Europe.…”

Section: Discussionmentioning

confidence: 99%

Muscovy Duck Parvovirus Infection with Epicarditis in Bali, Indonesia

Mahardika¹,

Putra²,

Dewi³

et al. 2015

J Veterinar Sci Technol

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Muscovy duck parvovirus (MDPV) is threatening industry and backyard Muscovy duck poultry throughout the world. Here, we confirmed its presence in Indonesia for the first time. The outbreak described in this study occurred in a small fatty liver (French: foie gras) industry in Tabanan, Bali, and affected ducklings aged 2-3 weeks. Although older Muscovy ducks were present at the facility, they did not present signs of illness. Among the ducklings, the morbidity and mortality rates were 100% and 90%, respectively. The initial incidence began on 15 June 2014 and ended a month later. It reoccurred in January 2015. Clinical signs were lethargy, anorexia, watery diarrhoea, and dyspnea. The most frequent pathological lesions were cardiac enlargement with pale pericardia, haemorrhage, and enlargement of the liver. Dominant histopathology features were severe enteritis, epicarditis, and hepatitis. Using polymerase chain reaction (PCR) and published primer pairs NS1/REP and VP1, MDPV infection was confirmed with whole DNA isolated from the heart and liver homogenates. Sequencing of the PCR products resulted in 900 bp NS1/REP and 1200 bp VP1 fragments specific to MDPV. The virus sequences from the two separated incidences were completely homologous with one another. Now that MDPV has been detected in Indonesia, it should be included in the differential diagnosis when evaluating ducklings with MDPV-associated symptoms.

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Section: Discussionmentioning

confidence: 99%

Muscovy Duck Parvovirus Infection with Epicarditis in Bali, Indonesia

Mahardika¹,

Putra²,

Dewi³

et al. 2015

J Veterinar Sci Technol

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“…MDPV causes high morbidity and mortality in ducklings under 3 weeks of age ( 40 , 58 , 65 ). The main clinical symptoms of this disease include wheezing, motor dysfunction and diarrhoea ( 31 ). In general, the mortality rate of infected Muscovy ducks over 3 weeks of age is much lower than that of younger birds, but survivors usually show symptoms of skeletal muscle degeneration and growth retardation ( 55 ).…”

Section: Introductionmentioning

confidence: 99%

Advances in research on genetic relationships of waterfowl parvoviruses

Chen

Afumba

Pang

et al. 2021

Journal of Veterinary Research

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Derzsy’s disease and Muscovy duck parvovirus disease have become common diseases in waterfowl culture in the world and their potential to cause harm has risen. The causative agents are goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), which can provoke similar clinical symptoms and high mortality and morbidity rates. In recent years, duck short beak and dwarfism syndrome has been prevalent in the Cherry Valley duck population in eastern China. It is characterised by the physical signs for which it is named. Although the mortality rate is low, it causes stunting and weight loss, which have caused serious economic losses to the waterfowl industry. The virus that causes this disease was named novel goose parvovirus (NGPV). This article summarises the latest research on the genetic relationships of the three parvoviruses, and reviews the aetiology, epidemiology, and necropsy characteristics in infected ducks, in order to facilitate further study.

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“…Live vaccines have been utilized in day-old Muscovy ducklings to prevent disease outbreaks. These attenuated vaccines were prepared by serial passages of the parental derivatives in cell cultures or embryonated eggs [ 15 – 17 ]. FZ91 is a virulent isolate obtained from deceased ducklings that died from an outbreak of “three-week” disease in a farm from Fuzhou city, China [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Construction and sequencing of an infectious clone of the goose embryo-adapted Muscovy duck parvovirus vaccine strain FZ91-30

Wang

Huang

Zhu

et al. 2016

Virol J

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BackgroundMuscovy duck parvovirus (MDPV) is the etiological agent of Muscovy duckling parvoviral disease, which is characterized by diarrhea, locomotive dysfunction, stunting, and death in young ducklings, and causes substantial economic losses in the Muscovy duck industry worldwide. FZ91-30 is an attenuated vaccine strain that is safe and immunogenic to ducklings, but the genomic information and molecular mechanism underlining the attenuation are not understood.MethodsThe FZ91-30 strain was propagated in 11-day-old embryonated goose eggs, and viral particles were purified from the pooled allantoic fluid by differential centrifugation and ultracentrifugation. Single-stranded genomic DNA was extracted and annealed to form double-stranded DNA. The dsDNA digested with NcoI resulted two sub-genomic fragments, which were then cloned into the modified plasmid pBluescript II SK, respectively, generating plasmid pBSKNL and pBSKNR. The sub-genomic plasmid clones were sequenced and further combined to construct the plasmid pFZ that contained the entire genome of strain FZ91-30. The complete genome sequences of strain FM and YY and partial genome sequences of other strains were retrieved from GenBank for sequence comparison. The plasmid pFZ containing the entire genome of FZ91-30 was transfected in 11-day-old embryonated goose eggs via the chorioallantoic membranes route to rescue infectious virus. A genetic marker was introduced into the rescued virus to discriminate from its parental virus.ResultsThe genome of FZ91-30 consists of 5,131 nucleotides and has 98.9 % similarity to the FM strain. The inverted terminal repeats (ITR) are 456 nucleotides in length, 14 nucleotides longer than that of Goose parvovirus (GPV). The exterior 415 nucleotides of the ITR form a hairpin structure, and the interior 41 nucleotides constitute the D sequence, a reverse complement of the D′ sequence at the 3′ ITR. Amino acid sequence alignment of the VP1 proteins between FZ91-30 and five pathogenic MDPV strains revealed that FZ91-30 had five mutations; two in the unique region of the VP1 protein (VP1u) and three in VP3. Sequence alignment of the Rep1 proteins revealed two amino acid alterations for FZ91-30, both of which were conserved for two pathogenic strains YY and P. Transfection of the plasmid pFZ in 11-day-old embryonated goose eggs resulted in generation of infectious virus with similar biological properties as compared with the parental strain.ConclusionsThe amino acid mutations identified in the VP1 and Rep1 protein may contribute to the attenuation of FZ91-30 in Muscovy ducklings. Plasmid transfection in embryonated goose eggs was suitable for rescue of infectious MDPV.

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Efficacy of a new attenuated duck parvovirosis vaccine in Muscovy ducks

Cited by 10 publications

References 21 publications

Muscovy Duck Parvovirus Infection with Epicarditis in Bali, Indonesia

Muscovy Duck Parvovirus Infection with Epicarditis in Bali, Indonesia

Advances in research on genetic relationships of waterfowl parvoviruses

Construction and sequencing of an infectious clone of the goose embryo-adapted Muscovy duck parvovirus vaccine strain FZ91-30

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