Efficacy of a new blocker against anti-ruthenium antibody interference in the Elecsys free triiodothyronine assay

Sapin, R; Agin, Arnaud; Gasser, F

doi:10.1515/cclm.2007.064

Cited by 21 publications

(17 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast, Elecsys ® FT 4 and FT 3 are competitive assays, where ruthenium-chelates are attached to a monomeric test antibody. Antibodies against such chelate-complexes were shown as a cause of analytical interference [38]. Antibody-ruthenium interactions were described mostly for FT 4 [31,37,[39][40][41] and FT 3 [37][38][39][40], but recently also for TSH [40], but never appearing as macro-TSH, as in our patient.…”

Section: Discussionsupporting

confidence: 55%

Anti-ruthenium antibodies mimic macro-TSH in electrochemiluminescent immunoassay

Gessl

Blueml

Bieglmayer

et al. 2014

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 55%

Anti-ruthenium antibodies mimic macro-TSH in electrochemiluminescent immunoassay

Gessl

Blueml

Bieglmayer

et al. 2014

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

show abstract

“…They include non-specific antibodies, often called heterophile antibodies, and other miscellaneous antibodies directed at reagent immunoglobulins (Kricka, 1999), biotin (Dale et al, 1994), or other epitopes (Levinson and Miller, 2002; Sapin et al, 2007). Other proteins in plasma that interfere with immunoassays and cause non-specific aggregation could also be included in this category simply due to our lack of biochemical understanding of these interferences.…”

Section: Immunoassays Are Imperfectmentioning

confidence: 99%

The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry

Hoofnagle

Wener

2009

Journal of Immunological Methods

484

330

View full text Add to dashboard Cite

Immunoassays have made it possible to measure dozens of individual proteins and other analytes in human samples for help in establishing the diagnosis and prognosis of disease. In too many cases the results of those measurements are misleading and can lead to unnecessary treatment or missed opportunities for therapeutic interventions. These cases stem from problems inherent to immunoassays performed with human samples, which include a lack of concordance across platforms, autoantibodies, anti-reagent antibodies, and the high-dose hook effect. Tandem mass spectrometry may represent a detection method capable of alleviating many of the flaws inherent to immunoassays. We review our understanding of the problems associated with immunoassays on human specimens and describe methodologies using tandem mass spectrometry that could solve some of those problems. We also provide a critical discussion of the potential pitfalls of novel mass spectrometric approaches in the clinical laboratory.

show abstract

“…The difference between our findings and the immunoassays in other laboratories led to the conclusion that the results were due to another interfering factor. Because the fT3 assay is known to be sensitive to antiruthenium antibodies, 5,6 the samples of these two women were analysed in Roche Diagnostics Laboratory. In both patients, an interference against ruthenium label was detected, explaining the interference in our fT3 and fT4 assays.…”

Section: Two Cases Of Antiruthenium Antibody Interference In Modular mentioning

confidence: 99%

Two cases of antiruthenium antibody interference in Modular free thyroxine assay

Heijboer¹,

IJzerman

Bouman³

et al. 2009

Ann Clin Biochem

View full text Add to dashboard Cite

to have a significant effect on the results. However, some kits provide prediluted standards and controls but samples require dilution.It may seem pedantic to insist upon the correct use of these mathematical terms, especially as in the examples seen a careful reading of the kit insert prevented an error. However, the potential for error exists and use of fractions and ratios as equivalent fosters misunderstanding about standard mathematical nomenclature that should be unambiguous and may have consequences elsewhere when correct use of these terms may be critical.

show abstract

Efficacy of a new blocker against anti-ruthenium antibody interference in the Elecsys free triiodothyronine assay

Cited by 21 publications

References 9 publications

Anti-ruthenium antibodies mimic macro-TSH in electrochemiluminescent immunoassay

Anti-ruthenium antibodies mimic macro-TSH in electrochemiluminescent immunoassay

The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry

Two cases of antiruthenium antibody interference in Modular free thyroxine assay

Contact Info

Product

Resources

About