Efficacy of a trans-sialidase-ISCOMATRIX subunit vaccine candidate to protect against experimental Chagas disease

Bontempi, Iván; Vicco, Miguel Hernán; Cabrera, Gabriel; Villar, Silvina Raquel; González, Florencia Belén; Roggero, Eduardo; Ameloot, Paul; Callewaert, Nico; Pérez, Ana Rosa; Marcipar, Iván Sergio

doi:10.1016/j.vaccine.2015.01.044

Cited by 39 publications

(43 citation statements)

References 54 publications

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“…Despite DHT as an estimation of TS-specific cellular response has same limitations (i.e., does not allow to recognize the T subpopulations involved in the specific response or the cytokines contributing in such reaction), the DHT assay continue to be one of the most rapid and available tests for the evaluation of this type of response during the screening of vaccine prototypes. As we have previously shown in other immunization schemes using TS ( Bontempi et al, 2015 , 2017 ; Bertona et al, 2017 ), it is expected that IFN-γ be one of the cytokines involved in this type of reaction.…”

Section: Discussionmentioning

confidence: 60%

Genetic Engineering of Lactococcus lactis Co-producing Antigen and the Mucosal Adjuvant 3′ 5′- cyclic di Adenosine Monophosphate (c-di-AMP) as a Design Strategy to Develop a Mucosal Vaccine Prototype

et al. 2018

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Lactococcus lactis is a promising candidate for the development of mucosal vaccines. More than 20 years of experimental research supports this immunization approach. In addition, 3′ 5′- cyclic di-adenosine monophosphate (c-di-AMP) is a bacterial second messenger that plays a key role in the regulation of diverse physiological functions (potassium and cellular wall homeostasis, among others). Moreover, recent studies showed that c-di-AMP has a strong mucosal adjuvant activity that promotes both humoral and cellular immune responses. In this study, we report the development of a novel mucosal vaccine prototype based on a genetically engineered L. lactis strain. First, we demonstrate that homologous expression of cdaA gen in L. lactis is able to increase c-di-AMP levels. Thus, we hypothesized that in vivo synthesis of the adjuvant can be combined with production of an antigen of interest in a separate form or jointly in the same strain. Therefore, a specifically designed fragment of the trans-sialidase (TScf) enzyme from the Trypanosoma cruzi parasite, the etiological agent of Chagas disease, was selected to evaluate as proof of concept the immune response triggered by our vaccine prototypes. Consequently, we found that oral administration of a L. lactis strain expressing antigenic TScf combined with another L. lactis strain producing the adjuvant c-di-AMP could elicit a TS-specific immune response. Also, an additional L. lactis strain containing a single plasmid with both cdaA and tscf genes under the Pcit and Pnis promoters, respectively, was also able to elicit a specific immune response. Thus, the current report is the first one to describe an engineered L. lactis strain that simultaneously synthesizes the adjuvant c-di-AMP as well as a heterologous antigen in order to develop a simple and economical system for the formulation of vaccine prototypes using a food grade lactic acid bacterium.

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Section: Discussionmentioning

confidence: 60%

Genetic Engineering of Lactococcus lactis Co-producing Antigen and the Mucosal Adjuvant 3′ 5′- cyclic di Adenosine Monophosphate (c-di-AMP) as a Design Strategy to Develop a Mucosal Vaccine Prototype

et al. 2018

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“…Lophiomeryx and Bachitherium are typical ruminants from the European Oligocene fauna. These genera appeared closely after the “Grande Coupure” event, during MP22 [ 8 ], [ 9 ], [ 87 ] and are probably part of the Asiatic immigrants ( Lophiomeryx has been found in Eocene Asiatic deposits, e. g. [ 63 ], [ 66 ], [ 67 ], [ 88 ], [ 89 ]). The last occurrence of these two genera in Europe corresponds to MP28.…”

Section: Discussionmentioning

confidence: 99%

“…An Asiatic origin for P . elongatum can be assumed, since this taxon has been already mentioned from Early Oligocene Asiatic localities (Kazakhstan, [ 66 ]; Mongolia, [ 90 ]; Georgia, [ 91 ]).…”

Section: Discussionmentioning

confidence: 99%

“…The specimen MNHN LIM148 is very interesting in completing the description of the central metacarpals attributed to a European Lophiomeryx , made by Brunet & Sudre ([ 29 ], USTL ACQ 328 from the old collection of Quercy) and by Schlosser ([ 65 ], Pl.2, Figs 13 & 14). MNHN LIM148 differs from the Early Oligocene from Mongolia Lophiomeryx gobiae described by Trofimov [ 66 ] in having shorter external metapodials II and V. However, Métais & Vislobokova [ 63 ] do not consider that Lophiomeryx gobiae belong to the genus Lophiomeryx and Vislobokova [ 67 ] proposed that this species is a Hypertragulidae. Nevertheless, the reduction of the lateral digits into bonny stylus appeared several times during the evolution of the ruminants [ 68 ].…”

Section: Systematic Paleontologymentioning

confidence: 99%

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The European Ruminants during the “Microbunodon Event” (MP28, Latest Oligocene): Impact of Climate Changes and Faunal Event on the Ruminant Evolution

Mennecart

2015

PLoS ONE

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The Earth already experienced numerous episodes of global warming and cooling. One of the latest impressive events of temperature rising was the Late Oligocene Warming that occurred around 25 Mya. An increase of the marine temperature of 2 to 4°C has been observed in a short time interval. In Europe, this major climatic event can be correlated to the continental faunal turnover “Microbunodon Event”. This event is marked by a huge faunal turnover (40% of the ungulate fauna during the first 500k years) and environmental changes. Drier conditions associated to the appearance of the seasonality lead to new environmental conditions dominated by wooded savannahs. This is correlated to a major arrival of Asiatic immigrants. Moreover, from a homogenous fauna during the main part of the Oligocene, local climatic variations between the European Western coast and the more central Europe could have provided faunal regionalism during the latest Oligocene and earliest Miocene. Considering the ruminants, this event is the major ever known for this group in Europe. A total renewal at the family level occurred. Thanks to a precise stratigraphic succession, major evolutionary elements are highlighted. Typical Oligocene species, mainly Tragulina, were adapted to wooded environments and were leaves/fruits eaters. They disappeared at the end of MP27 or the early MP28. This corresponds to the appearance of the Asiatic immigrants. The Tragulina (Lophiomerycidae, Bachitheriidae) and stem Pecora gave way to more derived stem and maybe crown Pecora (e.g. “Amphitragulus”, Babameryx, Dremotherium). These newcomers were adapted to more open environments and mixed feeding. The disappearance of the Tragulina is probably linked to environmental and vegetation changes, and competition. They give way to more derived ruminants having a more efficient metabolism in drier conditions and a better assimilation of less energetic food.

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“…All mTS-IMX immunized infected mice showed ~50 times less parasitemia than nonimmunized infected mice; in the chronic phase, tissue presented ~4.5 times lower parasite load. These results indicate that mTS-IMX formulation induces both optimal humoral and cellular immune responses, conferring protection against T. cruzi [ 32 ].…”

Section: Recombinant Protein Immunizationmentioning

confidence: 99%

Experimental Vaccines against Chagas Disease: A Journey through History

Rodríguez-Morales

Monteón-Padilla

Carrillo-Sánchez

et al. 2015

Journal of Immunology Research

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Chagas disease, or American trypanosomiasis, which is caused by the protozoan parasite Trypanosoma cruzi, is primarily a vector disease endemic in 21 Latin American countries, including Mexico. Although many vector control programs have been implemented, T. cruzi has not been eradicated. The development of an anti-T. cruzi vaccine for prophylactic and therapeutic purposes may significantly contribute to the transmission control of Chagas disease. Immune protection against experimental infection with T. cruzi has been studied since the second decade of the last century, and many types of immunogens have been used subsequently, such as killed or attenuated parasites and new DNA vaccines. This primary prevention strategy appears feasible, effective, safe, and inexpensive, although problems remain. The objective of this review is to summarize the research efforts about the development of vaccines against Chagas disease worldwide. A thorough literature review was conducted by searching PubMed with the terms “Chagas disease” and “American trypanosomiasis” together with “vaccines” or “immunization”. In addition, reports and journals not cited in PubMed were identified. Publications in English, Spanish, and Portuguese were reviewed.

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Efficacy of a trans-sialidase-ISCOMATRIX subunit vaccine candidate to protect against experimental Chagas disease

Cited by 39 publications

References 54 publications

Genetic Engineering of Lactococcus lactis Co-producing Antigen and the Mucosal Adjuvant 3′ 5′- cyclic di Adenosine Monophosphate (c-di-AMP) as a Design Strategy to Develop a Mucosal Vaccine Prototype

Genetic Engineering of Lactococcus lactis Co-producing Antigen and the Mucosal Adjuvant 3′ 5′- cyclic di Adenosine Monophosphate (c-di-AMP) as a Design Strategy to Develop a Mucosal Vaccine Prototype

The European Ruminants during the “Microbunodon Event” (MP28, Latest Oligocene): Impact of Climate Changes and Faunal Event on the Ruminant Evolution

Experimental Vaccines against Chagas Disease: A Journey through History

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