2017
DOI: 10.1038/s41598-017-14891-y
|View full text |Cite
|
Sign up to set email alerts
|

Efficacy of an Adenoviral Vectored Multivalent Centralized Influenza Vaccine

Abstract: Mice were immunized with Adenovirus expressing the H1-con, H2-con, H3-con and H5-con HA consensus genes in combination (multivalent) and compared to mice immunized with the traditional 2010–2011 FluZone and FluMist seasonal vaccines. Immunized mice were challenged with 10–100 MLD50 of H1N1, H3N1, H3N2 and H5N1 influenza viruses. The traditional vaccines induced robust levels of HA inhibition (HI) titers, but failed to protect against five different heterologous lethal influenza challenges. Conversely, the mult… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
19
0

Year Published

2019
2019
2023
2023

Publication Types

Select...
6
1

Relationship

2
5

Authors

Journals

citations
Cited by 23 publications
(19 citation statements)
references
References 40 publications
(41 reference statements)
0
19
0
Order By: Relevance
“…Co-administration of mosaic immunogens representing different influenza HA subtypes including H2, H3, and H5, as well as neuraminidase types N1 and N2, may have promise as universal influenza vaccine immunogens. Previous work has shown that multiple synthetic immunogens can be combined into a multivalent vaccine without losing efficacy of the individual components [15]. In this study we show that our mosaic immunogen is able to elicit broad levels of humoral and cellular immunity and cross-protection against divergent H1N1 influenza virus strains.…”
Section: Discussionmentioning
confidence: 63%
See 1 more Smart Citation
“…Co-administration of mosaic immunogens representing different influenza HA subtypes including H2, H3, and H5, as well as neuraminidase types N1 and N2, may have promise as universal influenza vaccine immunogens. Previous work has shown that multiple synthetic immunogens can be combined into a multivalent vaccine without losing efficacy of the individual components [15]. In this study we show that our mosaic immunogen is able to elicit broad levels of humoral and cellular immunity and cross-protection against divergent H1N1 influenza virus strains.…”
Section: Discussionmentioning
confidence: 63%
“…Recent studies have targeted the HA stalk region which is more conserved than the HA head domain [10][11][12][13]. Consensus HA approaches have also been investigated to maximize coverage for IAV strains [14,15]. HA and NA approaches rely on the induction of antibody responses to provide protection against IAV.…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, we vaccinated C57BL/6 mice with 10 10 vp of Adenovirus 4 and 5 vectors expressing a consensus H1 influenza hemagglutinin (HA) and sacrificed the mice 2 weeks later in order to evaluate the anti-HA antibody and T-cell response. The influenza HA protein is known to induce a strong humoral immune response when expressed in a viral vector making this an ideal transgene to study induction of antibody responses 17,40 . In support of this, we found that Ad5-HA vaccination resulted in significant anti-HA antibody response as measured by ELISA ( Fig.…”
Section: Adenovirus 4 Expressing Influenza Hemagglutinin Results In Nmentioning
confidence: 99%
“…Adenovirus (Ad) viral vectors have shown efficacy in preclinical and clinical trials against ZIKV, along with many other important infectious diseases such as influenza 17,18 , HIV 19,20 , and Ebola [21][22][23] . Traditionally, Adenovirus type 5 (Ad5) has been the most commonly used Ad vector.…”
mentioning
confidence: 99%
“…However, owing to the variability of influenza viruses, traditional vaccines do not provide effective immunity to evolutionary strains. 7 In 1976, Ebola was first discovered in Central Africa. Ebola hemorrhagic fever has been rampant in West Africa since 2014; it has spread rapidly to South America and Europe and is almost out of control.…”
Section: Emerging Infectious Diseasesmentioning
confidence: 99%