Efficacy of an anticoccidial live vaccine in prevention of necrotic enteritis in chickens

Bangoura, Berit; Alnassan, Alaa Aldin; Lendner, Matthias; Shehata, Awad A.; Krüger, Monika; Daugschies, Arwid

doi:10.1016/j.exppara.2014.08.004

Cited by 13 publications

(11 citation statements)

References 53 publications

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“…In contrast, IL-12 was upregulated by the end of the observation period in group CI, which might be related to the development of E. tenella meronts. Interestingly, in contrast to the cited in vivo studies [ 19 , 55 ], we did not observe a distinct increase in IL-12 mRNA expression in co-infected macrophages. Therefore, we assume that not macrophages but other immune cell populations are responsible for the previously observed in vivo increase of IL-12 expression.…”

Section: Discussioncontrasting

confidence: 99%

“…[ 19 ]. In addition, high expression of those cytokines was observed in chickens co-infected with E. tenella and Clostridium perfringens [ 55 ]. Based on our observations in the co-infection model, using 12 hour intervals for cytokine expression measurements might be useful in the future to more comprehensively judge parasite-host cell interactions.…”

Section: Discussionmentioning

confidence: 99%

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Mutual interactions of the apicomplexan parasites Toxoplasma gondii and Eimeria tenella with cultured poultry macrophages

et al. 2018

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BackgroundToxoplasma gondii and Eimeria tenella are two common parasites in poultry. Mixed infections are likely to occur frequently in chickens due to the high prevalence of both pathogens. In this study, we investigate the co-occurrence of the two pathogens in the same immunocompetent host cell population towards potential parasite-parasite as well as altered patterns of parasite-host interactions.MethodsPrimary macrophages from chicken blood were co-infected in vitro with T. gondii tachyzoites (RH strain) and E. tenella sporozoites (Houghton strain) for 72 h. Morphological observations by light microscopy and assessments of parasite replication by quantitative real-time PCR (qPCR) were performed at 24, 48 and 72 h post-infection (hpi). Six host cell immune factors previously linked to T. gondii or E. tenella infection were selected for gene expression analysis in this study.ResultsDistinct morphological changes of macrophages were observed during mixed infection at 24 hpi and immunological activation of host cells was obvious. Macrophage mRNA expression for iNOS at 48 hpi and for TNF-α at 72 hpi were significantly elevated after mixed infection. Distinct upregulation of IL-10 was also present during co-infection compared to Eimeria mono-infection at 48 and 72 hpi. At 72 hpi, the total number of macrophages as well as the number of both parasites decreased markedly. As measured by qPCR, E. tenella population tended to increase during T. gondii co-infection, while T. gondii replication was not distinctly altered.ConclusionsMutual interactions of T. gondii and E. tenella were observed in the selected co-infection model. The interactions are supposed to be indirect considering the observed changes in host cell metabolism. This study would thus help understanding the course of co-infection in chickens that may be relevant in terms of veterinary and zoonotic considerations.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mutual interactions of the apicomplexan parasites Toxoplasma gondii and Eimeria tenella with cultured poultry macrophages

et al. 2018

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“…Genes netB and tpeL were present in human isolated C. perfringens , but presence may not correlate with the virulence of NE [ 154 ]. The gene cpb2 has not been associated with virulence in broiler chickens as cpb2 -positive isolates from diseased birds failed to produce the CPB2 toxin [ 150 , 155 ]. Both NE and non-NE producing isolates have been found to contain cpb2 , suggesting that there is little association between this toxin and the disease process [ 135 , 150 ].…”

Section: C Perfringensmentioning

confidence: 99%

“…In 2017, 40% of US broiler integrators used coccidiosis vaccines (CV) in their programs, either incorporated as hybrids or stand-alone vaccination programs [ 28 ]. Live CV has shown to offer a protective effect for NE, reduce the severity of lesions, and lessen mortality associated with NE [ 10 , 32 , 155 , 178 ]. Common methods of vaccine application include intra-ocular administration, hatchery spray administration, edible gel placed on chick trays at hatchery or on feed trays, spray on feed administration, intra-yolk sac administration, and in ovo administration [ 179 ].…”

Section: Disease Prevention Strategies In Naementioning

confidence: 99%

“…Common methods of vaccine application include intra-ocular administration, hatchery spray administration, edible gel placed on chick trays at hatchery or on feed trays, spray on feed administration, intra-yolk sac administration, and in ovo administration [ 179 ]. CV aids in flock performance as it reduces the effects of Eimeria or C. perfringens infections on weight gain [ 155 ]. The use of CVs has also decreased the incidence of gangrenous dermatitis [ 180 ].…”

Section: Disease Prevention Strategies In Naementioning

confidence: 99%

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Avian Pathogenic Escherichia coli and Clostridium perfringens: Challenges in No Antibiotics Ever Broiler Production and Potential Solutions

et al. 2020

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United States is the largest producer and the second largest exporter of broiler meat in the world. In the US, broiler production is largely converting to antibiotic-free programs which has caused an increase in morbidity and mortality within broiler farms. Escherichia coli and Clostridium perfringens are two important pathogenic bacteria readily found in the broiler environment and result in annual billion-dollar losses from colibacillosis, gangrenous dermatitis, and necrotic enteritis. The broiler industry is in search of non-antibiotic alternatives including novel vaccines, prebiotics, probiotics, and housing management strategies to mitigate production losses due to these diseases. This review provides an overview of the broiler industry and antibiotic free production, current challenges, and emerging research on antibiotic alternatives to reduce pathogenic microbial presence and improve bird health.

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Parasitic Infections in Turkeys

Hernandez-Velasco,

Tellez-Isaias,

Hernandez-Patlan

et al. 2024

Turkey Diseases and Disorders Volume 2

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Efficacy of an anticoccidial live vaccine in prevention of necrotic enteritis in chickens

Cited by 13 publications

References 53 publications

Mutual interactions of the apicomplexan parasites Toxoplasma gondii and Eimeria tenella with cultured poultry macrophages

Mutual interactions of the apicomplexan parasites Toxoplasma gondii and Eimeria tenella with cultured poultry macrophages

Avian Pathogenic Escherichia coli and Clostridium perfringens: Challenges in No Antibiotics Ever Broiler Production and Potential Solutions

Parasitic Infections in Turkeys

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