Efficacy of antimicrobial combinations to reduce the use of sodium hypochlorite in the control of planktonic and sessile Escherichia coli

Meireles, Ana; Machado, Idalina; Fulgêncio, Rita; Mergulhão, Filipe; Melo, L. F.; Simões, Manuel

doi:10.1016/j.bej.2015.02.035

Cited by 16 publications

(10 citation statements)

References 65 publications

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“…According to previous studies, contaminated hospital surfaces are mostly colonized by monolayer adhered cells with densities of 10 4 -10 6 CFU/cm 2 (values in the range of those found in this study for Escherichia coli and Staphylococcus aureus) (Dancer, 2004;Wren et al 2008;Otter et al 2015). Moreover, it was found that the effects of selected disinfectants were similar on CFU reduction of monolayer adhered cells (2 h adhesion) and biofilms (24 h-old) (Meireles et al 2015).…”

Section: Discussionmentioning

confidence: 90%

Phytochemical profiling as a solution to palliate disinfectant limitations

et al. 2016

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Section: Discussionmentioning

confidence: 90%

Phytochemical profiling as a solution to palliate disinfectant limitations

et al. 2016

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“…Briefly, the 0.1 mL of the culture broth 101 without the microorganism growth was inactivated using 0.9 ml of the inactivation buffer (Lectin 10 g/L, Polyoxyethylene (20) sorbitan monooleate 30 g/L, L-histidine 1 g/L, Tryptone 1 g/L, Na2S2O3˙5H2O 20 g/L) and then inoculated the cells and plated on the TSA plate. The MBCs were determined by the lowest concentration of PAA that completely inhibited the M. psychrotolerans colony growth (Meireles et al, 2015).…”

Section: Evaluation Of Antibacterial Activity Of Paamentioning

confidence: 99%

Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine-producing bacterium, Morganella psychrotolerans

Wang

Yamaki

Kawai

et al. 2020

LWT

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In this study, we aimed to investigate the sanitizing effects of peracetic acid (PAA), and the underlying mechanism of its antimicrobial action against Morganella psychrotolerans. The exposure of M. psychrotolerans to 20 ppm PAA for 5 min led to its decrease below the detection level, indicating that PAA has a significant antibacterial effect against M. psychrotolerans in vitro. Moreover, the viable counts of M. psychrotolerans on saury (Cololabis saira) surface were showed a reduction of 1.40 and 2.23 log CFU/cm 2 upon treatment with 80 ppm PAA for 1 and 5 min, 18 respectively. Further, PAA treatment delayed the growth of M. psychrotolerans on 19 saury surface during storage at 4°C. Next, the antimicrobial mechanism of PAA against M. psychrotolerans cells was investigated, and the damage to the cell membrane and cell surface upon PAA treatment was observed using scanning electron microscopy (SEM) and epifluorescence microscopy. The chromosomal DNA and the protein profiles after PAA treatment were also analyzed. Form our results, we hypothesized that the bactericidal effect of PAA treatment was mainly attributed to damage the bacterial cell membrane. These results indicate that PAA may be an efficient disinfectant against M. psychrotolerans and has applications in seafood processing and storage.

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“…Duarte et al [17] found that the combined ultrasound and dichloroisocyanurate had significantly improved the antibacterial effect. In addition, ultrasound can improve the antibacterial effect of oregano essential oil and organic acids [18,19]. Phenyllactic acid (PLA) has a broad spectrum of effective antibacterial activity against many Gram-positive bacterial species, including S. aureus and Listeria monocytogenes, as well as Gram-negative bacterial species, including S. enteritidis and E. coli.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Antibiofilm Effects of Ultrasound and Phenyllactic Acid against Staphylococcus aureus and Salmonella enteritidis

Zhang

Wang

Sun

et al. 2021

Foods

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This study evaluated the effect of the combination of ultrasound and phenyllactic acid (PLA) on inactivating Staphylococcus aureus and Salmonella Enteritidis biofilm cells and determined the possible antibiofilm mechanism. S. aureus and S. Enteritidis biofilm cells were separately treated with ultrasound (US, 270 W), phenyllactic acid (PLA, 0.5% and 1%), and their combination (US + 0.5% PLA, and US + 1% PLA) for 5, 10, 20, 30, and 60 min. Biofilm inactivation, polysaccharide, and respiratory chain dehydrogenase assays were conducted. US and PLA had a synergistic effect on inactivating bacterial cells in S. aureus and S. Enteritidis biofilms. The combination of US and PLA significantly decreased the contents of soluble and insoluble polysaccharides and the activity of respiratory chain dehydrogenase in the biofilm cells compared to the single treatment. Confocal laser scanning microscopy, scanning electron microscopy, and intracellular adenosine-triphosphate (ATP) analyses indicated that the combination of US and PLA seriously destroyed the cell membrane integrity of the S. aureus and S. Enteritidis biofilms and caused the leakage of intracellular ATP. These findings demonstrated the synergistic antibiofilm effect of US combined with PLA and offered a research basis for its application in the food industry.

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Efficacy of antimicrobial combinations to reduce the use of sodium hypochlorite in the control of planktonic and sessile Escherichia coli

Cited by 16 publications

References 65 publications

Phytochemical profiling as a solution to palliate disinfectant limitations

Phytochemical profiling as a solution to palliate disinfectant limitations

Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine-producing bacterium, Morganella psychrotolerans

Synergistic Antibiofilm Effects of Ultrasound and Phenyllactic Acid against Staphylococcus aureus and Salmonella enteritidis

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