Efficacy of antimicrobial polymer coatings in an animal model of bacterial infection associated with foreign body implants

Hart, Emily; Azzopardi, Kristy; Taing, Heng; Graichen, Florian H. M.; Jeffery, Justine; Mayadunne, Roshan T. A.; Wickramaratna, Malsha; O’Shea, Mike; Nijagal, Brunda; Watkinson, R M; O’Leary, Stephen; Finnin, Barrie C.; Tait, Russell J.; Robins-Browne, Roy M.

doi:10.1093/jac/dkq057

Cited by 39 publications

(25 citation statements)

References 18 publications

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“…Currently, combination biomaterials and coatings are being developed for the treatment and prevention of biofilm‐related infections. The majority of animal studies that are used to model infections related to these materials primarily involve the use of an initial inoculum of planktonic bacterial cells from batch cultures 1–24. The expectation has been that these planktonic cells would attach to the surface of a biomaterial, medical device, or surrounding tissue and subsequently form a biofilm.…”

Section: The Use Of Planktonic Cells In Animal Modelsmentioning

confidence: 99%

Using biofilms as initial inocula in animal models of biofilm‐related infections

Williams

Costerton

2011

J Biomed Mater Res

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One of the most practical strategies that has been undertaken to fight biofilm implant-related infections has been the development of coatings on biomaterial devices that can elute antimicrobials into regions of patients' tissues. To date, the majority of animal studies that have been developed to model infections that accompany the use of these materials have primarily involved an initial inoculum of planktonic bacterial cells from batch cultures. Although valuable, data that have been derived from these experiments may not provide important clinical insight into how bacteria in well-established, mature biofilms impact device-related and other clinical infections when they contaminate a patient site or implanted device. In this review, a discussion is presented on the impact that a shift in biofilm research may have if initial inocula of well-established, mature biofilms are used to model biomaterial device-related infections in animal models.

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Section: The Use Of Planktonic Cells In Animal Modelsmentioning

confidence: 99%

Using biofilms as initial inocula in animal models of biofilm‐related infections

Williams

Costerton

2011

J Biomed Mater Res

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“…Quorum sensing, which depends on cell density, might explain colonization of catheters at a minimum dose of 10 8 bacteria, which seems to be the initial dose required for inducing effective biofilm formation in this model. Compared to doses of 10 6 , which often have been used in S. aureus animal models, the high dose of S. epidermidis used in our model is probably due to the fact that S. epidermidis lacks mass of virulence factors, which are present in S. aureus , and its versatile potential to evade host defense mechanisms [22], [23].…”

Section: Discussionmentioning

confidence: 99%

Catheter Colonization and Abscess Formation Due to Staphylococcus epidermidis with Normal and Small-Colony-Variant Phenotype Is Mouse Strain Dependent

Sander¹,

Börner²,

Kriegeskorte³

et al. 2012

PLoS ONE

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Coagulase-negative staphylococci (CoNS) form a thick, multilayered biofilm on foreign bodies and are a major cause of nosocomial implant-associated infections. Although foreign body infection models are well-established, limited in vivo data are available for CoNS with small-colony-variant (SCV) phenotype described as causative agents in implant-associated infections. Therefore, we investigated the impact of the Staphylococcus epidermidis phenotype on colonization of implanted PVC catheters and abscess formation in three different mouse strains. Following introduction of a catheter subcutaneously in each flank of 8- to 12-week-old inbred C57BL/6JCrl (B6J), outbred Crl:CD1(ICR) (CD-1), and inbred BALB/cAnNCrl (BALB/c) male mice, doses of S. epidermidis O-47 wild type, its hemB mutant with stable SCV phenotype, or its complemented mutant at concentrations of 106 to 109 colony forming units (CFUs) were gently spread onto each catheter. On day 7, mice were sacrificed and the size of the abscesses as well as bacterial colonization was determined. A total of 11,500 CFUs of the complemented mutant adhered to the catheter in BALB/c followed by 9,960 CFUs and 9,900 CFUs from S. epidermidis wild type in BALB/c and CD-1, respectively. SCV colonization was highest in CD-1 with 9,500 CFUs, whereas SCVs were not detected in B6J. The minimum dose that led to colonization or abscess formation in all mouse strains was 107 or 108 CFUs of the normal phenotype, respectively. A minimum dose of 108 or 109 CFU of the hemB mutant with stable SCV phenotype led to colonization only or abscess formation, respectively. The largest abscesses were detected in BALB/c inoculated with wild type bacteria or SCV (64 mm2 vs. 28 mm2). Our results indicate that colonization and abscess formation by different phenotypes of S. epidermidis in a foreign body infection model is most effective in inbred BALB/c followed by outbred CD-1 and inbred B6J mice.

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“…A 1.2 cm × 1.0 cm section was cut from the sheet for use in the phosphatase assay. Polylactic acid and dried PM4, at a mass ratio of 10:1 (typically 5 g total mass), was added to a laboratory micro extruder (barrel length 10 cm) that was equipped with a conventional single stage screw and extruded at a temperature of 190°C through a 0.5 mm die orifice 40. A 1.5 cm long piece of the resulting rod was used for the phosphatase assay.…”

Section: Methodsmentioning

confidence: 99%

New polymer syntheses part: 54 novel conducting polymers and copolymers based on 4‐Teriary butyl‐cyclohexanone moiety in the main chain

Aly

2011

J of Applied Polymer Sci

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A new interesting class of conducting polymer and copolymers based on 4-teriary butyl-cyclohexanone in the main chain has been synthesized by solution polycodensation of terephthalaldehyde with 4-teriary butylcyclohexanone and/or cycloalkanone derivatives. The model compound I was synthesized from the 4-teriary butyl-cyclohexanone with benzaldehyde, and its structure was confirmed by elemental and spectral analyses. The resulting polymer and copolymers were characterized by elemental and spectral analyses including Fourier transform infrared spectrometer (FT-IR) and nuclear magnetic resonance ( 1 H-NMR), beside solubility and viscometry measurements. The thermal properties of those polymer and copolymers were evaluated by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC) measurements and correlated to their structural units. X-ray analysis showed that it has some degree of crystallinity in the region 2y ¼ 5-60. The UV-visible spectra of some selected polymers were measured in dimethyl sulfoxide (DMSO) solution and showed absorption bands in the range 253-398 nm, due to n-p* and p-p* transition. The morphological properties of selected examples were tested by scanning electron microscope (SEM). Moreover, the electrical conductivities and the doping with iodine were tested.

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Efficacy of antimicrobial polymer coatings in an animal model of bacterial infection associated with foreign body implants

Abstract: Bioerodable polyester-polyurethane polymer coatings containing levofloxacin can prevent bacterial colonization of implants in an intra-operative model of device-related infections.

Cited by 39 publications

References 18 publications

Using biofilms as initial inocula in animal models of biofilm‐related infections

Using biofilms as initial inocula in animal models of biofilm‐related infections

Catheter Colonization and Abscess Formation Due to Staphylococcus epidermidis with Normal and Small-Colony-Variant Phenotype Is Mouse Strain Dependent

New polymer syntheses part: 54 novel conducting polymers and copolymers based on 4‐Teriary butyl‐cyclohexanone moiety in the main chain

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