Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Arafa, Mohamed; Agarwal, Ashok; Majzoub, Ahmad; Selvam, Manesh Kumar Panner; Baskaran, Saradha; Henkel, Ralf; Elbardisi, Haitham

doi:10.3390/antiox9030219

Cited by 60 publications

(64 citation statements)

References 56 publications

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“…While there are no studies to which we can directly compare our results, we can look to the many other studies employing antioxidant-based treatments for comparison. In most studies, employing an antioxidant cocktail (typically containing vitamins C and E, as well as zinc, selenium, l -carnitine and coQ10), or even individual antioxidants such as N -acetyl-cysteine, treatment for 2–3 months significantly reduced sperm DNA fragmentation measured by sperm chromatin structure assay (SCSA), sperm chromatin dispersion (SCD) or TUNEL [ 18 , 19 , 20 , 21 , 22 , 24 , 27 , 52 , 53 ]. In most cases, the average decrease following treatment was modest (<10%) [ 18 , 22 , 24 , 27 , 52 ], despite being statistically significant.…”

Section: Discussionmentioning

confidence: 99%

“…A considerable number of studies have investigated how treatment with an antioxidant cocktail, often based on vitamins C and E, impacts sperm DNA fragmentation [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. However, almost all of these studies were performed on a patient cohort which was not selected based on elevated DNA fragmentation.…”

Section: Introductionmentioning

confidence: 99%

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Mitigating the Effects of Oxidative Sperm DNA Damage

et al. 2020

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Sperm DNA damage is correlated with reduced embryo development and increased miscarriage risk, reducing successful conception. Given its links with oxidative stress, antioxidants have been investigated as a potential treatment, yet results are conflicting. Importantly, individual antioxidants are not identical in composition, and some compounds may be more effective than others. We investigated the use of the polyphenol-rich, high-antioxidant-capacity fruit acai as a treatment for elevated sperm DNA fragmentation (>16%), measured by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Following ≥ 74 days of treatment, we observed a significant decrease in sperm DNA fragmentation (−17.0% ± 2.5%) to 11.9 ± 1.7% (0–37%), with a 68.6% success rate (defined as post-treatment TUNEL < 16%). Post-treatment decreases in DNA fragmentation and success rates were not significantly impacted by low motility and/or concentration, or exceptionally high (> 25%) TUNEL. Treatment significantly reduced concentration in men with normal semen parameters, but 88% remained normal. Overall, successful treatment was not associated with age, semen parameters or TUNEL result at baseline. However, body mass index was significantly higher in nonresponders at baseline. This study provides evidence of a low-cost, effective treatment for elevated sperm DNA damage using acai.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mitigating the Effects of Oxidative Sperm DNA Damage

et al. 2020

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“…Reciente dos trabajos publicados por dos de los grupos más prestigiosos en los antioxidantes y la fertilidad 67,68 , confirman el papel importante que juegan los antioxidantes. El profesor Aitken 67 reafirma la importancia de controlar el estrés oxidativo en múltiples eventos, incluso algunos de estilo de vida modificables, lo cual permitir repercutir positivamente sobre la calidad seminal, mientras que el otro grupo demuestra que el tratamiento con antioxidantes durante tres meses mejora la calidad seminal, parámetros convencionales y funcionales, en hombres con infertilidad idiopática 68 .…”

Section: Discussionunclassified

Efecto de la borra de café sobre la biológica espermática: aproximación in vitro

Mesa

Maya

2020

Rev. chil. obstet. ginecol.

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RESUMEN Objetivo: El objetivo de este trabajo fue establecer el efecto de la borra de café sobre la movilidad y los parámetros funcionales de los espermatozoides humanos in vitro. Materiales y métodos: La borra de café, un subproducto obtenido en establecimientos especializados en la preparación de café soluble a base de grano, se diluyo en tampón fosfato salino y se mezcló en proporciones iguales con las muestras de semen de 16 voluntarios aparentemente sanos. A cada muestra se le determinó el efecto sobre la movilidad espermática en función del tiempo (30, 60, 90 y 120 minutos, n=16) y sobre los parámetros funcionales (n=6) por medio de citometría de flujo: potencial de membrana mitocondrial, producción de especies reactivas de oxígeno y lipoperoxidación de la membrana espermática. Resultados: La incubación de los espermatozoides con la borra de café evidencio un cambio positivo en la movilidad espermática. Adicionalmente, la incubación con la borra de café incremento significativamente el potencial de membrana mitocondrial en los espermatozoides. Conclusión: La borra de café, seguramente debido a los compuestos antioxidantes, afecta positivamente la movilidad espermática aumentando el potencial de membrana mitocondrial. Por lo tanto, esto es un paso inicial en la búsqueda de un suplemento de origen natural que aumente la calidad seminal.

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“…Несмотря на многочисленные исследования и достижения в диагностике мужского бесплодия, значительная часть случаев секреторной патозооспермии остается идиопатической. Идиопатическое мужское бесплодие характеризуется нарушением количественных и качественных параметров спермограммы при отсутствии факторов риска мужского бесплодия в анамнезе, нарушений по результатам объективного обследования, изменений эндокринных, генетических и биохимических показателей [3].…”

Section: Introductionunclassified

“…В связи с тем что одним из важных патогенетических механизмов, снижающих фертильность мужчин, является гиперпродукция активных форм кислорода, для лечения идиопатического мужского бесплодия часто применяют антиоксидантную терапию, которую проводят в течение минимум 3 мес. [3,6]. Несмотря на то что ряд исследований продемонстрировал эффективность данной группы препаратов, только 50 % мужчин, обратившихся за медицинской помощью в связи с идиопатическим бесплодием, можно помочь медикаментозно, тогда как остальные в большинстве случаев нуждаются в дорогостоящих процедурах вспомогательных репродуктивных технологий [7].…”

Section: Introductionunclassified

Our experience of application of low-level laser therapy in red spectrum in male idiopathic secretory infertility

Potapova

Кирилловна²,

Borovets

et al. 2020

Urology reports (St. - Petersburg)

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Introduction. Due to insufficient efficacy of medical therapy of idiopathic secretory male infertility there is a need to develop and apply more resultative methods of treatment. The aim of our study was to assess the efficacy of low-level laser therapy (LLLT) in red spectrum on sperm parameters, sperm DNA fragmentation (SDNAF) and MAR-test rate in men with idiopathic infertility. Patients and methods. 85 men with idiopathic infertility were examined, 50 of them (first group) were treated with LLLT and 35 (second group) didnt have course of LLLT. Average age of patients of the first and second groups was 31.4 1.3 and 32.3 1.4 years. LLLT was performed in red spectrum on a Rubin-C device (Russia). 10 laser therapy procedures were carried out every two days with active luminescence for 10 minutes of both testicles. Male hormone panel, semen analysis, MAR-test, SDNAF were assessed before and after the treatment. Results. LLLT in patients of the first group resulted in significant increase in ejaculate volume, sperm viability, number of morphologically normal sperm forms (by an average of 11, 9 and 23% respectively), and active mobile sperm forms by an average of 14 and 19% one and two months after the therapy respectively. In 12 patients of the first group with initially increased SDNAF its level became normal after the treatment. Significant decrease of MAR-test we searched out in patients of the first group with its initial level less than 30%. As a result of the treatment, pregnancies developed in 10 (20%) out of 50 couples in the first group. Conclusions. LLLT in red spectrum leads to significant improvement of sperm quality in patients with idiopathic infertility, normalizes sperm DNA-fragmentation level and decreases MAR-test level.

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Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility

Cited by 60 publications

References 56 publications

Mitigating the Effects of Oxidative Sperm DNA Damage

Mitigating the Effects of Oxidative Sperm DNA Damage

Efecto de la borra de café sobre la biológica espermática: aproximación in vitro

Our experience of application of low-level laser therapy in red spectrum in male idiopathic secretory infertility

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