Efficacy of Autologous Melanocyte Transplantation on Amniotic Membrane in Patients With Stable Leukoderma

Abstract

Vitiligo is a disfiguring disease with no definitive treatment options that significantly affects patients' quality of life. We aimed to compare, for what we believe to be the first time, the repigmentation efficacy of cultured epidermal cell suspension (CES) and amniotic membrane (AM)-cultured epidermal cell grafting (CEG) in the treatment of stable vitiligo. (December 15, 2010, to June 5, 2013, randomized, double-blind, intraindividually placebo-controlled clinical trial with a 6-month posttreatment follow-up period (last follow-up, November 26, 2012) was carried out in the dermatology department of the University Clinic of Navarra, Spain. The study was approved by the local institutional review board (Comité Ético de Investigación Clínica de Navarra, Health Department, Government of Navarra, Spain). Written informed consent was received from all patients. The participants did not receive financial compensation.Of 30 eligible patients with stable leukoderma, 24 individuals (15 women; age range, 18-57 years) were included in the final analyses. Dermatologic examination was performed on each patient to select one large vitiligo lesion (≥90 cm 2 ) or several smaller vitiligo lesions (up to 5 lesions, ≥90 cm 2 in total) per patient.Amniotic membranes were obtained during elective cesarean delivery as described. 1 Melanocyte growth medium M2 (M2; PromoCell) was used for the culture. A superficial shave biopsy (0.5 cm 2 ) was taken from pigmented buttock skin under local anesthesia. Epidermal cells were obtained (Dispase II neutral protease, grade II; Roche; and TrypLE Select enzyme; Gibco-Life Technologies) as described. 1 Cells were subcultured in two 75-cm 2 culture flasks. When 70% to 80% confluence was reached,

Use of platelet‐rich plasma to suspend noncultured epidermal cell suspension improves repigmentation after autologous transplantation in stable vitiligo: a double‐blind randomized controlled trial

“…On extensive literature search, we found only two double‐blind studies which evaluated cellular techniques in vitiligo surgery . Since ours is a split body double‐blind randomized controlled study, the chance of bias, and confounding factors is minimal.…”

Amniotic membrane and its epithelial and mesenchymal stem cells as an appropriate source for skin tissue engineering and regenerative medicine

“…This strategy is shown to be more effective than the routine therapeutic procedures such as surgery and cell-based therapies. Various ECM components of the AM's matrix such as collagen type IV and VII, laminins type 1 and type 5 have the main role in proliferation and adhesion of melanocytes [72,73]. Moreover, the AM has been successfully utilized in the regeneration of non-healing wounds in patients who suffer from epidermolysis bullosa.…”

Dermatopathology Trends in African Americans: A Retrospective Analysis of Biopsies

“…Moreover, other studies reporting the incidence of skin cancer in the general population had low representation of AA. 4 To our knowledge, how frequently AA patients are biopsied and the reason for the biopsy have not been performed. Having such information is important because it helps to inform the types of skin conditions for which AA are biopsied.…”