Efficacy of Bacillus Subtilis (GalliPro) supplementation in Clostridium perfringens challenged necrotic enteritis of broiler chicken

Abstract

The experiment was designed for 42 days to assess dietary supplementation of Bacillus Subtilis (GalliPro-DSM 17299) on growth performance and intestinal health against C. perfringens induced necrotic enteritis in broiler birds. Birds (n=400) were divided in to four groups [healthy (control), infected, healthy supplemented with B. subtilis @ 500 g/T of feed (1.2 x 10 6 cfu/g); infected supplemented with B. Subtilis@ 500 g/T ] with five replicates of 20 birds in each group. Necrotic enteritis was induced in broiler birds via inoculation of 50,000 oocysts of mixed strains of Eimeria species on 14 days of age followed by C. perfringens (10 7 cfu/mL) on 17, 18 and 19 days of age. Study revealed that necrotic enteritis challenge suppressed the body weight gain significantly, whereas infected birds supplemented with Bacillus subtilis showed significantly higher body weight gain. The feed intake and feed conversion ratio was not significantly affected in any of the groups. Significant reduction was noticed in bacterial count of C. perfringens infected birds supplemented with B. subtilis. Intestinal damage was observed in the infected control birds while it was recovered in birds supplemented with B. subtilis. The significant beneficial effect of B. Subtilis was recorded in the production performance of broiler chicken even in the absence of necrotic enteritis challenge. Thus it was concluded that supplementation of B. Subtilis helps to improve body weight gain and gut health to control necrotic enteritis.

Efficacy of Bacillus subtilis to replace in-feed antibiotics of broiler chickens under necrotic enteritis-challenged experiments: a systematic review and meta-analysis

“… 22 Cheng et al (2018) Taiwan Ross 308 Female 60 C. perfringens B. subtilis NIU068 Chiao-Xi hot spring in Taiwan 23 Wang et al (2018) USA Ross × Ross 708 Male 1344 Eimeria oocysts B. subtilis PB6 n.a. 24 Abudabos et al (2017) USA Ross 308 Mixed 480 C. perfringens B. subtilis (ATCC PTA-6737) Clostat, Kemin Industries Inc., Des Moines, IA 25 Koli et al (2018) India Vencobb- 400 Mixed 400 C. perfringens B. subtilis (GalliPro-DSM 17299) n.a. 26 Abudabos et al (2013) Saudi Arabia Ross 308 Male 100 C. perfringens B. subtilis PB6 (CloSTAT) Kemin Industries Inc., Des Moines, IA 27 Jayaraman et al (2013) India Cobb 400 Mixed 216 Eimeria sp.…”

Bacillus spp. Probiotic Strains as a Potential Tool for Limiting the Use of Antibiotics, and Improving the Growth and Health of Pigs and Chickens

“…The interaction between the Bacillus strains and the intestinal mucosa could also promote a local intestinal innate immune response in broilers derived from the host-bacteria cross-talk. For instance, according to Kazemi et al (2019;probiotic (Koli et al, 2018). In addition, the supplementation of B. subtilis DSM 17299 significantly improved the number of CD4 + in the ileum and cecum (+ 30% and + 50% as compared with the control group, respectively) of 7-day old broilers (Lourenco et al, 2012).…”

PENGARUH GLUKOMANAN PORANG DAN Bacillus Subtilis TERHADAP MORFOLOGI USUS DAN PRODUKTIVITAS AYAM BROILER

“…Beberapa penelitian menggunakan Bacillus subtilis dalam pakan dapat menurunkan populasi Salmonella dan Coliform serta meningkatkan produktivitas ayam broiler (Knap et al, 2011;Deniz et al, 2011;Harrington et al, 2016;Koli et al, 2017). Sheng-Qiu et al (2013) juga menyatakan bahwa Bacillus subtilis natto dapat memperbaiki morfologi usus dan menaikkan produktivitas bebek.…”