Efficacy of Bacillus tequilensis strain JN-369 to biocontrol of rice blast and enhance rice growth

Zhou, Huaqiang; Zhu, Huajun; Ren, Zhijun; Li, Xiaojuan; Zhong, Jie; Liu, Erming

doi:10.1016/j.biocontrol.2021.104652

Cited by 27 publications

(15 citation statements)

References 40 publications

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“…Bacillus tequilensis strain JN-369, isolated from xiangzaoxian no. 24 healthy rice plants, can promote seed germination and seedling growth, increase plant defense enzyme activities, reduce the severity of rice leaf blast and panicle blast, and increase rice yield . These reports show that B.…”

Section: Discussionmentioning

confidence: 83%

“…24 healthy rice plants, can promote seed germination and seedling growth, increase plant defense enzyme activities, reduce the severity of rice leaf blast and panicle blast, and increase rice yield. 25 These reports show that B. tequilensis has a wide application prospect in agriculture. However, there are few reports about VOC production by B. tequilensis.…”

Section: ■ Discussionmentioning

confidence: 99%

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Antibiotic Effects of Volatiles Produced by Bacillus tequilensis XK29 against the Black Spot Disease Caused by Ceratocystis fimbriata in Postharvest Sweet Potato

Guo

et al. 2021

J. Agric. Food Chem.

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Black spot disease caused by Ceratocystis fimbriata is destructive to the production, transportation, and storage of sweet potato. The antifungal effects of Bacillus tequilensis XK29 against C. fimbriata through volatile organic compounds (VOCs) were evaluated in this study. The activated carbon assay proved that XK29 could exert antibiotic effects through volatiles. By optimizing the wheat seed weight, inoculation method, concentration, volume, and time, the antifungal activity of XK29 was significantly improved. XK29 fumigation inhibited spore formation and germination and changed the cell morphology of C. fimbriata. During the storage of sweet potato tuber roots, XK29 effectively controlled black spot disease and reduced the weight loss and malondialdehyde content. Metabolomic analysis revealed that 21 volatile compounds were released from XK29. Isovaleric acid, isobutyric acid, and 2-methylbutanoic acid effectively inhibited the growth of C. fimbriata. These results indicate that B. tequilensis XK29 has a good potential to be developed as a microbial fumigation agent.

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Section: Discussionmentioning

confidence: 83%

Section: ■ Discussionmentioning

confidence: 99%

Antibiotic Effects of Volatiles Produced by Bacillus tequilensis XK29 against the Black Spot Disease Caused by Ceratocystis fimbriata in Postharvest Sweet Potato

Guo

et al. 2021

J. Agric. Food Chem.

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“…The occurrence of Bacillus tequilensis was also reported in other ecological niches like 2000‐years old shaft tomb (Gatson et al, 2006), mangrove soil (Sharifi & Kunchirman, 2021), saline water pond (Cortés‐Camargo et al, 2021), geothermal hot spring (Fachrial et al, 2020), soil crust (Zhao et al, 2019), coastal soil (Pathak et al, 2014), fermented goat milk (Abid et al, 2019), fermented soybean (Chaurasia et al, 2020), Thai milk kefir (Vijitra et al, 2018) and chicken gastrointestinal tract (Rani et al, 2017). The use of this strain as an inoculant is also reported on limited occasions (Kang et al, 2019; Pal et al, 2021; Zhou et al, 2021). Another opportunistic endophyte, B. kochii reported in the present work was first isolated from dairy and pharmaceutical production sites (Seiler et al, 2012) and later reported from soil (Liu et al, 2020), compost pit, coral reef, insect gut (Wan et al, 2017), fermented fish (Alvionita et al, 2020) and sparingly found as endophytes in tomato seed (Someya et al, 2021), papaya (Thomas et al, 2019) and noni (Liu et al, 2015b).…”

Section: Discussionmentioning

confidence: 93%

Authentication of putative competitive bacterial endophytes of rice by re-isolation and DNA fingerprinting assay

Devi

Balachandar

2022

Journal of Applied Microbiology

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Aim:The plant-growth-promoting putative competitive endophytes offer significant benefits to sustainable agriculture. The unworthy opportunistic and passenger endophytes are inevitable during the isolation of putative competitive endophytes.This study aimed to discriminate the putative competitive endophytes undoubtedly from the opportunistic and passenger endophytes. Methods and results:The newly isolated endophytes from field-grown rice were inoculated to 5-days old rice seedlings under gnotobiotic conditions. Re-isolation of the inoculated strains from the root surface, inner tissues of the whole plant, root and shoot was performed after 5-days. All the re-isolated colonies were compared with native isolates for homology by BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR) and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) DNA fingerprints. The results revealed that the putative competitive endophytes (RE25 and RE10) showed positive for re-isolation and BOX and ERIC fingerprints for the whole plant, root and shoot. The opportunistic (RE27 and RE8) and passenger endophytes (RE44 and RE18) failed in re-isolation either from root or shoot. The epiphytes (ZSB15 and Az204) showed negative for endophytic re-isolation and positive for surface colonization. Conclusion:This modified procedure can discriminate the putative competitive endophytes from others.Significance and impact of the study: Eliminating the opportunistic and passenger endophytes and epiphytes early by this method would help develop endophytic inoculants to enhance rice productivity.

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“…However, rice blast disease caused by Magnaporthe oryzae is a serious threat to rice production and can cause yield losses of 10%–35% per year ( Li et al, 2021 ). Currently, the main agents used to control rice blast are still chemical fungicides ( Zhou et al, 2021 ). However, the excessive use of such fungicides over the years is not only responsible for the development of resistance in the target fungal strains but also causes disruption of the microbial community, leading to decreased soil health ( Meyer et al, 2021 ) and not favoring the meeting of sustainable agricultural development goals.…”

Section: Introductionmentioning

confidence: 99%

Genomic and Biocontrol Potential of the Crude Lipopeptide by Streptomyces bikiniensis HD-087 Against Magnaporthe oryzae

Liu

Wang

et al. 2022

Front. Microbiol.

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Rice blast caused by Magnaporthe oryzae is one of the most destructive plant diseases. The secondary metabolites of Streptomyces have potential as biological control agents against M. oryzae. However, no commercial secondary antimicrobial products of Streptomyces have been found by gene prediction, and, particularly relevant for this study, a biocontrol agent obtained from Streptomyces bikiniensis has yet to be found. In this research, genomic analysis was used to predict the secondary metabolites of Streptomyces, and the ability to develop biocontrol pharmaceuticals rapidly was demonstrated. The complete genome of the S. bikiniensis HD-087 strain was sequenced and revealed a number of key functional gene clusters that contribute to the biosynthesis of active secondary metabolites. The crude extract of lipopeptides (CEL) predicted by NRPS gene clusters was extracted from the fermentation liquid of S. bikiniensis HD-087 by acid precipitation followed by methanol extraction, and surfactins, iturins, and fengycins were identified by liquid chromatography-mass spectrometry (LC–MS). In vitro, the CEL of this strain inhibited spore germination and appressorial formation of M. oryzae by destroying membrane integrity and through the leakage of cellular components. In vivo, this CEL reduced the disease index of rice blast by approximately 76.9% on detached leaves, whereas its control effect on leaf blast during pot experiments was approximately 60%. Thus, the S. bikiniensis CEL appears to be a highly suitable alternative to synthetic chemical fungicides for controlling M. oryzae.

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Efficacy of Bacillus tequilensis strain JN-369 to biocontrol of rice blast and enhance rice growth

Cited by 27 publications

References 40 publications

Antibiotic Effects of Volatiles Produced by Bacillus tequilensis XK29 against the Black Spot Disease Caused by Ceratocystis fimbriata in Postharvest Sweet Potato

Antibiotic Effects of Volatiles Produced by Bacillus tequilensis XK29 against the Black Spot Disease Caused by Ceratocystis fimbriata in Postharvest Sweet Potato

Authentication of putative competitive bacterial endophytes of rice by re-isolation and DNA fingerprinting assay

Genomic and Biocontrol Potential of the Crude Lipopeptide by Streptomyces bikiniensis HD-087 Against Magnaporthe oryzae

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