Efficacy of Bacterial Cellulose as a Carrier of BMP-2 for Bone Regeneration in a Rabbit Frontal Sinus Model

Kitahara, Takashi; Sha, Jingjing; Bai, Yunpeng; Matsuda, Yuichi; Hideshima, Katsumi; Yamada, Teppei; Kanno, Takahiro

doi:10.3390/ma12152489

Cited by 20 publications

(16 citation statements)

References 49 publications

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“…The results of this process were vinegar and nanobiocellulose biomass. Bacterial cellulose is produced by the Gram-negative bacterium Gluconacetobacter xylinus and extracted from the culture medium in a pure 3D structure, formed by an ultra-thin network of highly hydrated (3–8 nm) cellulose nanofibers (99% in weight), displaying high molecular weight, high cellulose crystallinity (60–90%), enormous mechanical strength, and full biocompatibility [ 18 , 20 , 22 , 23 , 24 , 25 , 26 , 27 ].…”

Section: Methodsmentioning

confidence: 99%

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Is the Bacterial Cellulose Membrane Feasible for Osteopromotive Property?

et al. 2020

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Guided bone regeneration was studied to establish protocols and develop new biomaterials that revealed satisfactory results. The present study aimed to comparatively evaluate the efficiency of the bacterial cellulose membrane (Nanoskin®) and collagen membrane Bio-Gide® in the bone repair of 8-mm critical size defects in rat calvaria. Seventy-two adult male rats were divided into three experimental groups (n = 24): the CG—membrane-free control group (only blood clot, negative control), BG—porcine collagen membrane group (Bio-Guide®, positive control), and BC—bacterial cellulose membrane group (experimental group). The comparison periods were 7, 15, 30, and 60 days postoperatively. Histological, histometric, and immunohistochemical analyses were performed. The quantitative data were subjected to 2-way ANOVA and Tukey’s post-test, and p < 0.05 was considered significant. At 30 and 60 days postoperatively, the BG group showed more healing of the surgical wound than the other groups, with a high amount of newly formed bone (p < 0.001), while the BC group showed mature connective tissue filling the defect. The inflammatory cell count at postoperative days 7 and 15 was higher in the BC group than in the BG group (Tukey’s test, p = 0.006). At postoperative days 30 and 60, the area of new bone formed was greater in the BG group than in the other groups (p < 0.001). Immunohistochemical analysis showed moderate and intense immunolabeling of osteocalcin and osteopontin at postoperative day 60 in the BG and BC groups. Thus, despite the promising application of the BC membrane in soft-tissue repair, it did not induce bone repair in rat calvaria.

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Section: Methodsmentioning

confidence: 99%

“…Cellulose membranes remain in the body for a long time without being degraded [ 11 ], but their contact with tissues does not generate toxicity or inflammation [ 11 , 17 , 18 ]. Bacterial cellulose is produced by strains of Gram-negative bacteria and is quite different from plant cellulose [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Is the Bacterial Cellulose Membrane Feasible for Osteopromotive Property?

et al. 2020

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“…Gene expression studies have been developed to ensure successful tissue regeneration, such as using BC to release BMP-2 and to promote optimal tissue formation. Interestingly, the BC + BMP-2 combination enhanced bone regeneration and appeared to be a promising clinical approach [ 43 ]. These tools may help preserve the native phenotype, which is considered a complex challenge in the field.…”

Section: Discussionmentioning

confidence: 99%

Beneficial Roles of Cellulose Patch-Mediated Cell Therapy in Myocardial Infarction: A Preclinical Study

Simeoni

Mogharbel²,

Francisco

et al. 2021

Cells

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Biological scaffolds have become an attractive approach for repairing the infarcted myocardium and have been shown to facilitate constructive remodeling in injured tissues. This study aimed to investigate the possible utilization of bacterial cellulose (BC) membrane patches containing cocultured cells to limit myocardial postinfarction pathology. Myocardial infarction (MI) was induced by ligating the left anterior descending coronary artery in 45 Wistar rats, and patches with or without cells were attached to the hearts. After one week, the animals underwent echocardiography to assess for ejection fraction and left ventricular end-diastolic and end-systolic volumes. Following patch formation, the cocultured cells retained viability of >90% over 14 days in culture. The patch was applied to the myocardial surface of the infarcted area after staying 14 days in culture. Interestingly, the BC membrane without cellular treatment showed higher preservation of cardiac dimensions; however, we did not observe improvement in the left ventricular ejection fraction of this group compared to coculture-treated membranes. Our results demonstrated an important role for BC in supporting cells known to produce cardioprotective soluble factors and may thus provide effective future therapeutic outcomes for patients suffering from ischemic heart disease.

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“…Thus, Boyne and James [106] created the maxillary sinus lifting technique by performing a graft, allowing implant insertion. In this way, Koike et al [107] performed frontal bone defects in 12 rabbits that were divided in 4 groups: BC (BC grafting only), BMP-2 (treated only with BMP-2 solution), and BC + BMP-2 (BC loaded with BMP-2 graft). As a result, BC maintained the graft space and BMP- At the current stage of development, the efforts of the most recent research seem to be focused on the development of synthetic bone substitutes that are able to mimic extracellular matrix functions of natural tissues and lead the host response, offering similar results to the autograft implants [98,99].…”

Section: Bc In Dentistrymentioning

confidence: 99%

“…Thus, Boyne and James [ 106 ] created the maxillary sinus lifting technique by performing a graft, allowing implant insertion. In this way, Koike et al [ 107 ] performed frontal bone defects in 12 rabbits that were divided in 4 groups: BC (BC grafting only), BMP-2 (treated only with BMP-2 solution), and BC + BMP-2 (BC loaded with BMP-2 graft). As a result, BC maintained the graft space and BMP-2 was released in a controlled manner into the target area, showing that BC + BMP-2 is a promising option to increase bone structure and for the placement of dental implants.…”

Section: Bc In Dentistrymentioning

confidence: 99%

Bacterial Nanocellulose in Dentistry: Perspectives and Challenges

Barud¹,

Silva

Borges

et al. 2020

Molecules

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Bacterial cellulose (BC) is a natural polymer that has fascinating attributes, such as biocompatibility, low cost, and ease of processing, being considered a very interesting biomaterial due to its options for moldability and combination. Thus, BC-based compounds (for example, BC/collagen, BC/gelatin, BC/fibroin, BC/chitosan, etc.) have improved properties and/or functionality, allowing for various biomedical applications, such as artificial blood vessels and microvessels, artificial skin, and wounds dressing among others. Despite the wide applicability in biomedicine and tissue engineering, there is a lack of updated scientific reports on applications related to dentistry, since BC has great potential for this. It has been used mainly in the regeneration of periodontal tissue, surgical dressings, intraoral wounds, and also in the regeneration of pulp tissue. This review describes the properties and advantages of some BC studies focused on dental and oral applications, including the design of implants, scaffolds, and wound-dressing materials, as well as carriers for drug delivery in dentistry. Aligned to the current trends and biotechnology evolutions, BC-based nanocomposites offer a great field to be explored and other novel features can be expected in relation to oral and bone tissue repair in the near future.

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Efficacy of Bacterial Cellulose as a Carrier of BMP-2 for Bone Regeneration in a Rabbit Frontal Sinus Model

Cited by 20 publications

References 49 publications

Is the Bacterial Cellulose Membrane Feasible for Osteopromotive Property?

Is the Bacterial Cellulose Membrane Feasible for Osteopromotive Property?

Beneficial Roles of Cellulose Patch-Mediated Cell Therapy in Myocardial Infarction: A Preclinical Study

Bacterial Nanocellulose in Dentistry: Perspectives and Challenges

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