Efficacy of bovicin <scp>HC</scp>5 and nisin combination against <i>Listeria monocytogenes</i> and <i>Staphylococcus aureus</i> in fresh cheese

Pìmentel-Filho, Natan de Jesus; Mantovani, Hilário Cuquetto; Carvalho, Antônio Fernandes de; Dias, Ricardo Souza; Vanetti, Maria Cristina Dantas

doi:10.1111/ijfs.12316

Cited by 38 publications

(25 citation statements)

References 42 publications

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“… and Pimentel‐Filho et al . (). GCPHN were inoculated with 1 × 10 4 cfu/g of L. monocytogenes , then packed tightly in plastic bags and kept at 4C for 20 days.…”

Section: Methodsmentioning

confidence: 97%

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Antilisterial and Antioxidant Activities of Neutrase-Treated Grass Carp Proteins and their Effects on the Storage and Quality Properties of Fresh Noodle

Xiao

Niu

2016

Journal of Food Processing and Preservation

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This study primarily aimed to prepare grass carp proteins hydrolysates (GCPH) by Neutrase hydrolysis, equipping with antimicrobial and antioxidant activities. In addition, the effects of GCPH on inhibiting the growth of Listeria monocytogenes, the production of total volatile base nitrogen (TVB-N) and thiobarbituric acid reactive substances (TBARs) during storage, as well as the color, cooking quality, texture properties and amino acid were evaluated in fresh noodle. The results indicated that GCPH at hydrolysis degree of 19% (NSH19), showed the highest antilisterial (64.8%) and antioxidant (55.7%) activities. The water absorption and stability of wheat dough were increased with the increase content of NSH19. Furthermore, adding NSH19 can significantly achieve 20 days shelf life for fresh noodle inoculated with 10 4 cfu/g of L. monocytogenes at 4C, and produce lower TVB-N and TBARs content. Besides, adding NSH19 can improve the textural, cooking quality and amino acids pattern of fresh noodle. PRACTICAL APPLICATIONSAs the staple foods, traditional noodles lacking essential amino acids, are not perceived by the consumer. Grass carp is one of farm-raised freshwater fish, whose production comprises up to 35-40% (3.7 million tons annually) of the total freshwater fish species. However, the development of grass carp has not been fully exploited yet. This work demonstrated that GCPH could be used as natural additives in noodle formulations to promote consumer health and improve the shelf life, which is a win-win situation for the noodles and grass carp industry.

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“… and Pimentel‐Filho et al . (). GCPHN were inoculated with 1 × 10 4 cfu/g of L. monocytogenes , then packed tightly in plastic bags and kept at 4C for 20 days.…”

Section: Methodsmentioning

confidence: 97%

“…The experiment was determined according to Esmail Abdollahzadeh et al 2014 andPimentel-Filho et al (2014). GCPHN were inoculated with 1 3 10 4 cfu/g of L. monocytogenes, then packed tightly in plastic bags and kept at 4C for 20 days.…”

Section: Antilisterial Effect Of Gcph In the Gcphn During Storagementioning

confidence: 99%

Antilisterial and Antioxidant Activities of Neutrase-Treated Grass Carp Proteins and their Effects on the Storage and Quality Properties of Fresh Noodle

Xiao

Niu

2016

Journal of Food Processing and Preservation

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“…Among these strategies, bacteriocins have been proposed as a potentially useful alternative to control clinical pathogens, such as methicillin-resistant S. aureus (MRSA), Enterococcus faecalis, Listeria monocytogenes , and Clostridium difficile (22–24). The lantibiotics, a subgroup of bacteriocins characterized by the presence of post-translationally modified amino acid residues (ex.…”

Section: Introductionmentioning

confidence: 99%

Nisin penetratesStaphylococcus aureusbiofilms but shows differences in killing effects against sessile and planktonic cells

Godoy-Santos

Pitts

Stewart

et al. 2018

Preprint

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17Biofilms may restrict antimicrobial penetration and contribute to the recalcitrance of 18 bacterial infections. In this work, we investigated the penetration of nisin into S. aureus 19 biofilms and compared the susceptibility of S. aureus planktonic and sessile cells to this 20 lantibiotic. Biofilms were grown under continuous flow in CDC reactors and calcein 21 fluorescence was used to monitor the effect of nisin on the cytoplasmic membrane of S. 22 aureus cells. Confocal scanning laser microscopy (CLSM) showed that calcein was lost 23 within approximately 20 min in CDC biofilms, demonstrating that nisin penetrated to the 24 bottom of the biofilm and caused membrane permeabilization. Viability analysis using PI 25 staining showed that nisin was bactericidal against S. aureus sessile cells. Time-kill 26 assays were performed against S. aureus in the following conditions: homogenized 27 exponential planktonic (HEP), homogenized stationary planktonic (HSP), homogenized 28 CDC biofilm (HB) and intact CDC biofilm (IB). The mean viability reduction of HEP and 29 HSP were 6.71 and 1.64 log CFU.ml -1 , respectively, confirming that stationary S. aureus 30 cells were much less susceptible than exponential cells. The HB and IB treatments 31 showed mean viability reductions of 1.25 and 0.50 log CFU.ml -1 , respectively. Nisin 32 activity against S. aureus was not limited by its ability to penetrate the bacterial biofilm, 33 but the killing efficacy of the antimicrobial peptide was reduced by the physiological 34 status of the biofilm-grown cells. 35 36 37 38 3 Importance 39Biofilms represent a major problem to control microorganisms in industrial environments 40 and medical devices. We developed a direct real-time microscopic visualization 41 technique to demonstrate experimentally that the antimicrobial peptide nisin is able to 42 penetrate S. aureus biofilms. Our results confirmed that nisin caused membrane 43 permeabilization of sessile bacteria and revealed qualitative agreement between 44 viability loss and membrane integrity loss. This approach could improve the evaluation 45 of antibacterial susceptibility breakpoints when testing the efficacy of standard and 46 novel antimicrobials. 47 48

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“…Este resultado demonstra que L. monocytogenes pode se desenvolver em nata durante o período de armazenamento e chegar a concentrações celulares elevadas, mesmo quando o produto apresenta baixas concentrações iniciais (10 2 UFC.g -1 ) do micro-organismo. Resultado semelhante foi relatado por Pimentel-Filho et al (2014), que inocularam L. monocytogenes em queijo Minas Frescal, numa concentração inicial de 10 3 UFC.g -1 e, após 12 dias de armazenamento a 8-10°C, a concentração desse micro-organismo atingiu 10 8 UFC.g -1 .…”

Section: Introductionunclassified

Avaliação da multiplicação de Listeria monocytogenes e da atividade antimicrobiana do Biomax D® em nata

Biasus¹,

Chaves²,

Silva³

et al. 2016

Rev. Ciênc. Agr.

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R E S U M OAvaliou-se a multiplicação de Listeria monocytogenes em nata, um derivado lácteo com alto teor de gordura, bem como a atividade antilisteria do Biomax D ® neste alimento. A atividade antilisteria do Biomax foi comparada com a da nisina. Foram utilizados seis tratamentos: T1: nata + 10 2 UFC.g -1 de L. monocytogenes; T2: nata + 10 2 UFC.g -1 de L. monocytogenes + 2,5ppm de nisina; T3: nata + 10 2 UFC.g -1 de L. monocytogenes + 1,5% de Biomax; T4: nata + de 10 5 UFC.g -1 de L. monocytogenes; T5: nata + de 10 5 UFC.g -1 de L. monocytogenes + 2,5ppm de nisina; T6: nata + de 10 5 UFC.g -1 de L. monocytogenes + 1,5% de Biomax. A contagem de L. monocytogenes foi realizada em três tempos de armazenamento a 8°C (zero, 6º, 14° e 20° dia), com médias variando entre 2 e 10 log UFC.g -1 nos tratamentos sem adição de nisina e Biomax, entretanto, houve eliminação do micro-organismo nos tratamentos adicionados dos antimicrobianos. Verificou-se que L. monocytogenes se desenvolve em nata, podendo atingir elevados níveis de contaminação, mesmo quando apresentava baixas contagens iniciais. Além disso, observou-se que Biomax possui atividade antilisteria semelhante à nisina neste derivado lácteo, apresentando potencial para ser utilizado como antimicrobiano natural para o controle de L. monocytogenes em alimentos gordurosos. Palavras-chave: antimicrobianos, Biomax D ® , nata, nisina. A B S T R A C TThe proliferation of Listeria monocytogenes in cream, a milk derivative with high fat content, was evaluated as well as the anti-listerial activity of Biomax D ® in this type of food. Antilisterial activity of Biomax was compared with nisin. Six treatments were used: T1: Cream + 10 2 CFU.g -1 of L. monocytogenes; T2: Cream + 10 2 CFU.g -1 L. monocytogenes + 2,5ppm nisin; T3: Cream + 10 2 CFU.g -1 L. monocytogenes + 1.5% Biomax; T4: Cream + 10 5 CFU.g -1 of L. monocytogenes; T5: Cream + 10 5 CFU.g -1 L. monocytogenes + 2,5ppm nisin; T6: Cream + 10 5 CFU.g -1 L. monocytogenes + 1.5% Biomax. L. monocytogenes counts were performed on three days storage at 8 °C (zero, 6, 14 and 20 th day), with averages ranging between 2 and 10 log CFU.g -1 in treatments without the addition of nisin or Biomax ® . However, there was elimination of the microorganism when the antimicrobials were added. It was found that L. monocytogenes is able to grow in cream, reaching high levels of contamination, even when it presents low initial counts. In this dairy derivative, Biomax D ® presents antilisterial activity similar to nisin and might be used as a natural antibiotic for the control of L. monocytogenes in fatty foods.

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Efficacy of bovicin HC5 and nisin combination against Listeria monocytogenes and Staphylococcus aureus in fresh cheese

Cited by 38 publications

References 42 publications

Antilisterial and Antioxidant Activities of Neutrase-Treated Grass Carp Proteins and their Effects on the Storage and Quality Properties of Fresh Noodle

Antilisterial and Antioxidant Activities of Neutrase-Treated Grass Carp Proteins and their Effects on the Storage and Quality Properties of Fresh Noodle

Nisin penetratesStaphylococcus aureusbiofilms but shows differences in killing effects against sessile and planktonic cells

Avaliação da multiplicação de Listeria monocytogenes e da atividade antimicrobiana do Biomax D® em nata

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