Efficacy of bronchial brushings and trans-bronchial needle aspiration in diagnosing carcinoma lung

Gaur, DS; Kusum, Anuradha; Harsh, Meena; Kohli, Supreethi; Kishore, Sanjeev; Pathak, V. P.

doi:10.4103/0970-9371.42091

Cited by 16 publications

(12 citation statements)

References 10 publications

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“…Patients in this study were slightly younger with average age 60 in female and 63 in male. The youngest was 39-year-old patient, similar to data in previous investigations 12 . Although the gender distribution of lung cancer patients is equal in developed countries, there were three times more male than female in this study, as it was in other developing countries 10,11,13,14 .…”

Section: Discussionsupporting

confidence: 89%

Correlation between cytological and histopathological diagnosis of non-small cell lung cancer and accuracy of cytology in the diagnosis of lung cancer

et al. 2022

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Background/Aim. Lung cancer is one of the most common cancer types worldwide. More than 70% of patients are diagnosed in advanced stages of disease, with limited therapeutic options based on cytological and histopathological material. The value of cytology in diagnosing and subtyping of non-small cell lung cancer is very important for modern personalized therapies. The aim of this study is to find out the concordance between cytological and histopathological diagnosis of non-small cell lung cancer, and the accuracy, sensitivity, specificity, positive and negative predictive value of cytology in diagnosing lung cancer. Methods. Two-year retrospective study included 169 patients with cytological diagnosis of non-small cell lung cancer, histopathological small biopsy and surgical specimens for diagnosis confirmation, that were copmpared. Histopathological diagnosis on surgical specimens was golden standard for evaluation concordance to cytological diagnosis of non-small cell lung cancer and evaluation accuracy, specificity, sensitivity, positive and negative prognostic value of cytology as diagnostic method for detecting lung cancer. Results. This study included 76.3 % (129/169) male and 23.7% (40/169) female, age between 39 and 83, average 62.53?7.6. There was no statistically significant difference between ages of different genders (p=0.207). The most frequent among cytological diagnosis was non-small cell lung cancer in 58.58% (99/169) patients. Concordance between cytological and histopathological diagnoses of surgical specimens was 61.48%. There was no statistically significant difference between cytological diagnoses and histopathological diagnoses of small biopsies specimens (p=0.856). Sensitivity, specificity, positive and negative prognostic value and accuracy of cytology as diagnostic method of lung cancer were 94.98%, 98.60%, 95.72%, 98.35% and 97.71%, respectively. Conclusion. Cytological diagnosis of non-small cell lung cancer is accurate, with high sensitivity, specificity, and benefits for patients. Most patients are diagnosed in advanced stage of cancer when there is no surgical therapy option and the only available diagnostic material is small biopsy sampled during bronchoscopy.

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Section: Discussionsupporting

confidence: 89%

Correlation between cytological and histopathological diagnosis of non-small cell lung cancer and accuracy of cytology in the diagnosis of lung cancer

et al. 2022

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“…This result was also in agreement with other studies. [11][12][13] While Shah et al 27 reported the mean age of their patients with bronchial carcinoma was 45.6 years which was lower than the present study and Santos-Martínez et al 14 reported that the mean age was 67.1 (± 11.1), which was higher than the present study In this study 252 (84%) patients were male and 48 (16%) patients were female. Male were more frequently affected by bronchial carcinoma than that of female.…”

Section: Discussioncontrasting

confidence: 57%

Role of Brush Cytology in Diagnosis of Bronchial Carcinoma: Experience in Rajshahi Medical College Hospital

Islam¹,

Sani²,

Majumdar³

et al. 2020

TAJ: J of Teachers Assoc

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This was a descriptive study conducted in Rajshahi Medical College Hospital (RMCH), Rajshahi and the study period was from June 2017 to June 2019. A total number of 300 patients (48 females and 252 males) were enrolled who underwent elective fiber optic bronchoscopy with brush cytology for the diagnosis of bronchial carcinoma. Our aim was to identify the role of brush cytology in diagnosis of bronchial carcinoma. Most of the cases (84%) were male. Majority 111(37.00%) of the patients were in age group 60-72 years; the mean±SD age was (58.70±13.11). Most of the study participants were farmer 134(44.67%). 182(66.67%) were smoker and 118(39.33%) were non-smoker. For smokers, pack year history was <10 pack in 78(42.85%), 10-20 pack 66(36.26%) and >20 pack 38(20.87%) of cases. Squamous cell carcinoma was the most common type of bronchial carcinoma; found in 95 (31.66%) cases followed by adenocarcinoma 50 (16.66%), large cell carcinoma 43 (14.33%), small cell carcinoma 25 (8.33%), others 87 (29%) This study revealed that the bronchial brush cytology was minimally invasive, well enduring technique with high sensitivity in diagnosis of central lesion of bronchial carcinoma. TAJ 2019; 32(2): 1-6

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“…Bronchial brushing (BB) is a well-established method of obtaining airway cells for analysis by dislodging them with a brush in conjunction with fiberoptic bronchoscopy. 8 Although BB samples are a valuable source of primary airway cells for research, the sample size is typically very limited (0.8–3.6 × 10 4 cells). 9 The small sample size is further complicated by the specimen heterogeneity which in addition to the intact airway epithelia contains mucus, stromal tissue, and the remnants of cells killed during dislodging.…”

Section: Introductionmentioning

confidence: 99%

Measurement of Protein Tyrosine Phosphatase Activity in Single Cells by Capillary Electrophoresis

et al. 2013

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A fluorescent peptide substrate was used to measure dephosphorylation by protein tyrosine phosphatases (PTP) in cell lysates, and single cells and to investigate the effect of environmental toxins on PTP activity in these systems. Dephosphorylation of the substrate by PTPN1 and PTPN2 obeyed Michaelis-Menten kinetics, with KM values of 770 ± 250 nM and 290 ± 54 nM, respectively. Dose-response curves and IC50 values were determined for the inhibition of these two enzymes by the environmental toxins Zn2+ and 1,2-naphthoquinone, as well as pervanadate. In A431 cell lysates, the reporter was a poor substrate for peptidases (degradation rate of 100 ± 8.2 fmol min−1 mg−1) but an excellent substrate for phosphatases (dephosphorylation rate of 1.4 ± 0.3 nmol min−1 mg−1). Zn2+, 1,2-naphthoquinone and pervanadate inhibited dephosphorylation of the reporter in cell lysates with IC50 values of 470 nM, 35 μM, and 100 nM, respectively. Dephosphorylation of the reporter following loading into living single cells occurred at rates of at least 2 pmol min−1 mg−1. When single cells were exposed to 1,2-naphthoquinone (50 μM), Zn2+ (100 μM), and pervandate (1 mM), dephosphorylation was inhibited with median values and first and third quartile values of 41 (Q1 = 0%, Q3 = 96%), 50 (Q1 = 46%, Q3 = 74%), and 53% (Q1 = 36%, Q3 = 77%), respectively, demonstrating both the impact of these toxic exposures on cell signaling and the heterogeneity of response between cells. This approach will provide a valuable tool for the study of PTP dynamics, particularly in small, heterogeneous populations such as human biopsy specimens.

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Efficacy of bronchial brushings and trans-bronchial needle aspiration in diagnosing carcinoma lung

Cited by 16 publications

References 10 publications

Correlation between cytological and histopathological diagnosis of non-small cell lung cancer and accuracy of cytology in the diagnosis of lung cancer

Correlation between cytological and histopathological diagnosis of non-small cell lung cancer and accuracy of cytology in the diagnosis of lung cancer

Role of Brush Cytology in Diagnosis of Bronchial Carcinoma: Experience in Rajshahi Medical College Hospital

Measurement of Protein Tyrosine Phosphatase Activity in Single Cells by Capillary Electrophoresis

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