Efficacy of chemical disinfectants against snakehead rhabdovirus

Abstract

The susceptibility of snakehead rhabdovirus to treatment at 20 "C with 5 commercially available disinfectants was examined. No reduction in virus infectivity occurred followin exposure to 5 ppm malachite green for 6 hours. Treatment of infective ceII culture fluids with 2 % formalin resulted in > 99.9% reduction in virus titre within 5 minutes and complete inactivation within 30 minutes, but a negli ible loss in infectivity after exposure to 0.025% formalin for 1 hour. Suspensions of virus in distded water were completely inactivated within 5 minutes by 12.5 ppm chlorine, 50 pm iodine, or a 1 : 2000 dilution of a peroxygen disinfectant. In the presence of serum in infective cei culture fluids, however, > 50 pm chlorine was required to inactivate the agent and no measurable reduction in infectivity was ogserved following treatment with 500 ppm iodine for 30 minutes.

Susceptibility of salmonid alphavirus to a range of chemical disinfectants

“…The detrimental effect of the presence of organic matter on the efficacy of iodophor disinfectants has been shown previously for a number of other fish viruses (Elliott & Amend 1978; Frerichs 1990; Frerichs, Tweedie, Starkey & Richards 2000). The results presented here for Buffodine with SAV (Table 5) are consistent with these earlier studies.…”

Usage of electrolytic water system in the giant freshwater prawn,Macrobrachium rosenbergii(de Man) larval hatchery system

“…Electrolytic oxidizing water was proposed for a variety of applications, including fighting against microorganisms in medical environments, disinfection of drinking water, sanitization of public facilities and in agriculture (Frerichs ; Hayashibara et al . ; Fujiwara et al .…”

“…Electrolytic oxidizing water was proposed for a variety of applications, including fighting against microorganisms in medical environments, disinfection of drinking water, sanitization of public facilities and in agriculture (Frerichs 1990;Hayashibara et al 1994;Fujiwara et al 2000;Shiba et al 2000;Kim et al 2001;Park et al 2002;Fabrizio & Cutter 2005). Although several studies evaluated the potential for the use of EO water in aquaculture (Lin & Wu 1996;Yoshimizu, Ishikawa, Hori, Yoshinaka, Ezura, Watanabe & Uenishi 1998;Jorquera et al 2002;Wijesekara, Nomura & Matsumura 2006;Katayose et al 2007;Taparhudee, Suksamran, Srisapoome & Chuchird 2008;Díaz et al 2011), there are few reports on the effects of processing factors on the performance of EO water production.…”

“…Chlorine in EO water has a high efficiency as a disinfectant (Fabrizio & Cutter 2005), and in its various forms is widely used for microbiological control. Laboratory and field studies demonstrated the high biocidal efficiency of chlorine against viruses and bacterial pathogens (Frerichs 1990;Inouye, Ikeya, Yamazaki & Hara 1990;Pascho, Landolt & Ongerth 1995). Chlorine does not affect flora or fauna at permitted levels, whereas excess amounts of chlorine in aquatic environments can harm aquatic plants and animals.…”

Balıkların Önemli Viral Hastalıkları

“…Güneydoğu Asya'daki sıcak su balıklarını etkilemektedir [31]. SHRV hakkında Sri-Lanka'da yapılan bir araştırmaya [17] göre hastalığın enfektif alanda yaşama, etkeni barındıran su ile temas ve hastalıklı balıkların popülasyonda bulundurulması ile bulaştığı gösterilmiştir.…”