Efficacy of Chlorhexidine Mouthrinses With and Without Alcohol: A Clinical Study

Borrajo, José Luis Leyes; Varela, Luis García; Castro, Gonzalo López; Rodríguez-Núñez, Ignacio; Figueroa, M.; Torreira, Mercedes Gallas

doi:10.1902/jop.2002.73.3.317

Cited by 83 publications

(75 citation statements)

References 16 publications

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“…Chlorhexidine is a biocide used extensively as a topical antiseptic in hospitals (11) and for treatment of periodontal diseases (28). Chlorhexidine has a broad spectrum of activity against a variety of organisms, including C. albicans.…”

mentioning

confidence: 99%

Action of Chlorhexidine Digluconate against Yeast and Filamentous Forms in an Early-StageCandida albicansBiofilm

Suci

Tyler

2002

Antimicrob Agents Chemother

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An in situ method for sensitive detection of differences in the action of chlorhexidine against subpopulations of cells in Candida albicans biofilms is described. Detection relies on monitoring the kinetics of propidium iodide (PI) penetration into the cytoplasm of individual cells during dosing with chlorhexidine. Accurate estimation of the time for delivery of the dosing concentration to the substratum was facilitated by using a flow cell system for which transport to the interfacial region was previously characterized. A model was developed to quantify rates of PI penetration based on the shape of the kinetic data curves. Yeast were seeded onto the substratum, and biofilm formation was monitored microscopically for 3 h. During this period a portion of the yeast germinated, producing filamentous forms (both hyphae and pseudohyphae). When the population was subdivided on the basis of cell morphology, rates of PI penetration into filamentous forms appeared to be substantially higher than for yeast forms. Based on the model, rates of penetration were assigned to individual cells. These data indicated that the difference in rates between the two subpopulations was statistically significant (unpaired t test, P < 0.0001). A histogram of rates and analysis of variance indicated that rates were approximately equally distributed among different filamentous forms and between apical and subapical segments of filamentous forms.

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mentioning

confidence: 99%

Action of Chlorhexidine Digluconate against Yeast and Filamentous Forms in an Early-StageCandida albicansBiofilm

Suci

Tyler

2002

Antimicrob Agents Chemother

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“…The authors demonstrated that a statistically significant lower biofilm accumulation resulted when chlorhexidine was used in comparison to the placebo, regardless of the presence of alcohol in the chlorhexidine solutions. Zimmer et al and Leyes Borrajo et al found similar results 7,8 . However, tooth brushing was allowed in both studies, thus preventing direct comparison of their results with ours.…”

Section: Discussionmentioning

confidence: 62%

“…Conversely, Lorenz et al failed to demonstrate differences between alcohol-free and alcohol-containing rinses for both plaque and gingival bleeding index, in a 21-day experimental gingivitis study 6 . Other studies that compared alcohol-free and alcohol-containing chlorhexidine solutions, as adjuncts to tooth brushing for 4 weeks or more, failed to find differences in plaque and gingival bleeding index 7,8 . It may be hypothesized that chlorhexidine may inhibit subgingival biofilm buildup by inhibiting supragingival biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Chlorhexidine with or without alcohol against biofilm formation: efficacy, adverse events and taste preference

et al. 2017

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In recent years, different chlorhexidine formulations have been tested, including an alcohol-free alternative, but the effect of this solution on early biofilm formation is not clear. A crossover, randomized, double-blind clinical trial was conducted to evaluate the effect of two chlorhexidine solutions against supra-and subgingival biofilm formation (NCT#02656251). Thirty-five participants were randomized and asked to rinse twice daily with 15 ml of an alcohol-containing 0.12% chlorhexidine solution, an alcohol-free 0.12% chlorhexidine solution, or placebo. The study was conducted in three experimental periods of 4 days each, with a 10-day washout between the periods. All the experimental periods followed the same protocol, except that the solutions were switched. Biofilm distribution was evaluated every 24 hours by the Plaque-Free Zone Index, during 96 hours. Adverse events were self-reported and sensory evaluation was performed using a hedonic scale. Compared to the placebo, the chlorhexidine solutions resulted in a significantly higher number of surfaces free of plaque over 96 hours (p < 0.01), and were able to prevent subgingival biofilm formation (p < 0.01). The alcohol-free chlorhexidine solution was associated with a lower incidence of adverse events, compared with alcohol-containing chlorhexidine (p < 0.05); it also received better sensory evaluation and acceptance by trial participants, compared with the alcohol-containing chlorhexidine (p = 0.007), and had a similar inhibitory effect on the formation of supra-and subgingival biofilms.

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“…Therefore, herbal medicines which are naturally occurring provide most dependable and reliable results for restoration of oral and systemic health [12]. Antimicrobial mouthwashes used in periodontics have either sugar or alcohol in one of their contents [15]. These components increase the possibility of having caries and halitosis.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the efficacy of triphala mouth rinse as coadjuvant in the treatment of chronic generalized periodontitis: a randomized clinical trial

Irfan¹,

Kumar²,

Amin³

et al. 2017

Mouth Teeth

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Efficacy of Chlorhexidine Mouthrinses With and Without Alcohol: A Clinical Study

Abstract: In this study, the alcohol-free rinse was as effective as one containing alcohol in controlling plaque and reducing gingival inflammation. Therefore, it would seem that its use can be recommended in all patients, but especially in patients for whom the use of alcohol is contraindicated.

Cited by 83 publications

References 16 publications

Action of Chlorhexidine Digluconate against Yeast and Filamentous Forms in an Early-StageCandida albicansBiofilm

Action of Chlorhexidine Digluconate against Yeast and Filamentous Forms in an Early-StageCandida albicansBiofilm

Chlorhexidine with or without alcohol against biofilm formation: efficacy, adverse events and taste preference

Evaluation of the efficacy of triphala mouth rinse as coadjuvant in the treatment of chronic generalized periodontitis: a randomized clinical trial

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